The New Therapeutic Concept of Using a Rho Kinase Inhibitor for the Treatment of Corneal Endothelial Dysfunction

Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Cornea (Impact Factor: 2.04). 10/2011; 30 Suppl 1(Suppl 1):S54-9. DOI: 10.1097/ICO.0b013e3182281ee1
Source: PubMed


Corneal endothelial cells (CECs) show poor regenerative ability in humans, and noncompensatory damage of CECs causes irreversible corneal haziness in cases of bullous keratopathy. Although corneal transplantations provide considerable clinical benefits, allograft rejection, primary graft failure, and the shortage of donor corneas are problems that still need to be overcome. The establishment of new treatment therapies is the key to solving these problems, and we have attempted to establish a new clinical intervention for corneal endothelial dysfunction. We previously demonstrated that the inhibition of Rho/Rho kinase (ROCK) signaling by Y-27632, a specific ROCK inhibitor, promoted cell adhesion, inhibited apoptosis, and enhanced cell proliferation in cultured primate CECs. These results raise the possibility that the ROCK inhibitor might serve as a new tool for establishing an effective culture method for newly emerging cultivated CEC transplantation therapies. Moreover, because Y-27632 enhances cell proliferation in vitro, we hypothesized that the use of a ROCK inhibitor could be a new pharmacological intervention for the treatment of corneal endothelial dysfunction. We demonstrated that the topical instillation of a ROCK inhibitor promotes corneal endothelium wound healing in an animal model. This indicates that use of a ROCK inhibitor is a less invasive and novel therapy that should prove promising for the treatment of corneal endothelial dysfunction.

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Available from: Naoki Okumura, Jul 07, 2015
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    • "When HCE cells are damaged due to various causes (e.g., viral infection, intraocular surgery, or Fuchs dystrophy), corneal endothelial dysfunction occurs. Currently, such damage can be repaired only with corneal transplantation [3-5], although a specific Rho/Rho kinase inhibitor has potential for the treatment of corneal endothelial dysfunction [6]. Unfortunately, a very limited number of healthy donor corneas are available for corneal transplantation worldwide [7]. "
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