ABCA1 gene promoter DNA methylation is associated with HDL particle profile and coronary artery disease in familial hypercholesterolemia

Department of Biochemistry, Université de Sherbrooke, Sherbrooke, QC, Canada.
Epigenetics: official journal of the DNA Methylation Society (Impact Factor: 4.78). 05/2012; 7(5):464-72. DOI: 10.4161/epi.19633
Source: PubMed


High-density lipoproteins cholesterol (HDL-C) level, a strong coronary artery disease (CAD) clinical biomarker, shows significant interindividual variability. However, the molecular mechanisms involved remain mostly unknown. ATP-binding cassette A1 (ABCA1) catalyzes the cholesterol transfer from peripheral cells to nascent HDL particles. Recently, a differentially methylation region was identified in ABCA1 gene promoter locus, near the first exon. Therefore, we hypothesized that DNA methylation changes at ABCA1 gene locus is one of the molecular mechanisms involved in HDL-C interindividual variability. The study was conducted in familial hypercholesterolemia (FH), a monogenic disorder associated with a high risk of CAD . Ninety-seven FH patients (all p.W66G for the LDLR gene mutation and not under lipid-lowering treatment) were recruited and finely phenotyped for DNA methylation analyses at ABCA1 gene locus. ABCA1 DNA methylation levels were found negatively correlated with circulating HDL-C (r = -0.20; p = 0.05), HDL2-phospholipid levels (r = -0.43; p = 0.04), and with a trend for association with HDL peak particle size (r = -0.38; p = 0.08). ABCA1 DNA methylation levels were also found associated with prior history of CAD (CAD = 40.2% vs. without CAD = 34.3%; p = 0.003). These results suggest that epigenetic changes within the ABCA1 gene promoter contribute to the interindividual variability in plasma HDL-C concentrations and are associated with CAD expression. These findings could change our understanding of the molecular mechanisms involved in the pathophysiological processes leading to CAD.

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Available from: Luigi Bouchard, Oct 13, 2015
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    • "Moreover, methylation alterations of several other factors were found in blood samples. For example, ATP-binding cassette transporter A1 (ABCA1) promoter hypermethylation was positively associated with coronary artery disease (CAD), age and lipid profiles in leukocytes [125] [126]. "
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    • "PCR and sequencing primers were selected using the PyroMark Assay Design v2.0.1.15 (Qiagen), as previously described [7] (Additional file 1: Figure S1). DNA methylation levels were measured at eight CpG dinucleotides upstream from the first exon of the ABCA1 gene (ABCA1-CpG1 to -CpG8; Additional file 1: Figure S1). "
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    ABSTRACT: Background Previous studies have suggested that DNA methylation contributes to coronary artery disease (CAD) risk variability. DNA hypermethylation at the ATP-binding cassette transporter A1 (ABCA1) gene, an important modulator of high-density lipoprotein cholesterol and reverse cholesterol transport, has been previously associated with plasma lipid levels, aging and CAD, but the association with CAD has yet to be replicated. Results ABCA1 DNA methylation levels were measured in leucocytes of 88 men using bis-pyrosequencing. We first showed that DNA methylation at the ABCA1 gene promoter locus is associated with aging and CAD occurrence in men (P < 0.05). The latter association is stronger among older men with CAD (≥61 years old; n = 19), who showed at least 4.7% higher ABCA1 DNA methylation levels as compared to younger men with CAD (<61 years old; n = 19) or men without CAD (n = 50; P < 0.001). Higher ABCA1 DNA methylation levels in older men were also associated with higher total cholesterol (r = 0.34, P = 0.03), low-density lipoprotein cholesterol (r = 0.32, P = 0.04) and triglyceride levels (r = 0.26, P = 0.09). Furthermore, we showed that acetylsalicylic acid therapy is associated with 3.6% lower ABCA1 DNA methylation levels (P = 0.006), independent of aging and CAD status of patients. Conclusions This study provides new evidence that the ABCA1 epigenetic profile is associated with CAD and aging, and highlights that epigenetic modifications might be a significant molecular mechanism involved in the pathophysiological processes associated with CAD. Acetylsalicylic acid treatment for CAD prevention might involve epigenetic mechanisms.
    Full-text · Article · Jul 2014 · Clinical Epigenetics
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    • "Since various trails had been keen on the single-nucleotide polymorphisms, some had averted their sight to epigenetics, such as miRNA, DNA methylation. Recently, we have found that aberrant methylation is interpreted to take part in the occurrence and development of diseases including colorectal cancer [14, 15], breast cancer [16, 17], coronary artery disease [18], and schizophrenia [19]. For clopidogrel resistance, numerous studies have investigated the underlying mechanism. "
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