Isolation and molecular characterization of Candida africana from Jos, Nigeria

* Department of Medical Microbiology, University of Jos , Jos , Nigeria.
Medical mycology: official publication of the International Society for Human and Animal Mycology (Impact Factor: 2.34). 03/2012; 50(7):765-7. DOI: 10.3109/13693786.2012.662598
Source: PubMed


During a survey of the prevalence of Candida spp. in Jos, Plateau State, Nigeria, two atypical C. albicans isolates were recovered. These two yeasts were germ tube positive, chlamydospore-negative and gave a green color on CHROMagar Candida. Molecular analysis performed by amplification of the hwp1 gene showed that these two isolates belonged to C. africana, a newly proposed Candida species closely related to C. albicans. Based on the presence or absence of an intron in DNA sequences encoding rRNA, the two C. africana, including all C. albicans isolates examined, were found to belong to genotype A and no other genotypes or species such as C. dubliniensis were found. To our knowledge, this is the first isolation of C. africana in Nigeria.

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Available from: Giuseppe Criseo
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    • "Currently, only few molecular methods have been reported for specific identification of C. africana (Table 2). One of these, has been widely used in several epidemiological studies (Albaina et al., 2015; Gumral et al., 2011; Ngouana et al., 2015; Nnadi et al., 2012; Romeo and Criseo, 2009b; Shan et al., 2014; Sharma et al., 2014) and it is a simple PCR-based method that uses only a single pair of primer specifically designed to span a highly polymorphic region within the hyphal wall protein 1 (HWP1) gene (Romeo and Criseo, 2008). This method allows one to identify all members of the C. albicans complex in a single PCR reaction because it produces different size amplicons (C. "
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    • "No C. dubliniensis, C. inconspicua, and D. hansenii isolates were derived from DGP ICUs (Table 2) and none of these strains were found in DGP-specimens, except DGP swabs (Table 2). Gumral et al. [78] reported the lack of C. dubliniensis and C. africana strains in Turkey with vaginal C. albicans isolates, whilst Nnadi et al. [79] found in Nigeria no C. dubliniensis in vulvovaginal samples. As three C. africana isolates appeared in Berlin and two in Munich they were tested during a MCS in 2000, together with the strains from Angola and Madagascar. "
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