Mass spectral measurements of the apoHDL in horse (Equus caballus) cerebrospinal fluid

ArticleinComparative Biochemistry and Physiology Part D Genomics and Proteomics 7(2):172-4 · February 2012with9 Reads
Impact Factor: 2.06 · DOI: 10.1016/j.cbd.2012.02.002 · Source: PubMed

    Abstract

    As a continuation of our proteogenomic studies of equine apolipoproteins, we have obtained molecular masses for several of the apolipoproteins associated with the HDL in horse cerebrospinal fluid (CSF). Using electrospray-ionization mass spectrometry (ESI-MS), we report on values for apolipoproteins, A-I and A-II, as well as acylated apoA-I. In comparison with our previously published data on equine plasma apolipoproteins, there appears to be a higher percentage of acylated apoA-I in the CSF than in plasma. As was the case in plasma, apoA-II circulates as a homodimer. These studies also revealed a protein with a mass of 34,468Da that we are speculating is the value for horse apoE.