Production of human lactoferrin in animal milk 1 1 This article is part of a Special Issue entitled Lactoferrin and has undergone the Journal's usual peer review process.

Institute of Gene Biology, Russian Academy of Sciences, 119334, Vavilova str, 34/5, Moscow, Russia.
Biochemistry and Cell Biology (Impact Factor: 2.15). 02/2012; 90(3):513-9. DOI: 10.1139/o11-088
Source: PubMed


Genetic constructs containing the human lactoferrin (hLf) gene were created within a joint program of Russian and Belorussian scientists. Using these constructs, transgenic mice were bred (the maximum hLf concentration in their milk was 160 g/L), and transgenic goats were also generated (up to 10 g/L hLf in their milk). Experimental goatherds that produced hLf in their milk were also bred, and the recombinant hLf was found to be identical to the natural protein in its physical and chemical properties. These properties included electrophoretic mobility, isoelectric point, recognition by polyclonal and monoclonal antibodies, circular dichroic spectra, interaction with natural ligands (DNA, lipopolysaccharides, and heparin), the binding of iron ions, the sequence of the 7 terminal amino acids, and its biological activity. The latter was assessed by the agglutination of Micrococcus luteus protoplasts, bactericidal activity against Escherichia coli and Listeria monocytogenes , and fungicidal activity against Candida albicans . We also demonstrated a significant increase in the activity of antibiotics when used in combination with Lf.

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    • "Another study investigated the effects of rhLF on anemia and found that rhLF can improve iron status of rats with anemia more than supplementation with ferrous lactate alone (Wang et al. 2012). Lactoferrin is an antimicrobial protein with in vitro bactericidal activity against Escherichia coli and Listeria monocytogenes, and fungicidal activity against Candida albicans, as well as the ability to increase in the activity of antibiotics when used in combination in vitro (Goldman et al. 2012). Using an in vivo model, rhLF beneficially modulated the intestinal flora composition and improved the growth of young pigs (Hu et al. 2012). "
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