Article

Intestinal methane production in obese individuals is associated with a higher body mass index

GI Motility Program, Cedars-Sinai Medical Center, Los Angeles, California, USA.
Gastroenterology and Hepatology 01/2012; 8(1):22-8.
Source: PubMed

ABSTRACT

Obesity is an epidemic that affects 1 in 3 individuals in the United States, and recent evidence suggests that enteric microbiota may play a significant role in the development of obesity. This study evaluated the association between methanogenic archaea and obesity in human subjects.
Subjects with a body mass index (BMI) of 30 kg/m² or higher were prospectively recruited from the weight loss program of a tertiary care medical center. Subjects who met the study's inclusion criteria were asked to complete a questionnaire that included a series of visual analogue scores for bowel symptom severities. Subjects then provided a single end-expiratory breath sample to quantitate methane levels. Bivariate and multivariate analyses were used to determine associations with BMI.
A total of 58 patients qualified for enrollment. Twenty percent of patients (n = 12) had breath test results that were positive for methane (>3 parts per million [ppm]), with a mean breath methane concentration of 12.2±3.1 ppm. BMI was significantly higher in methane-positive subjects (45.2±2.3 kg/m²) than in methane-negative subjects (38.5±0.8 kg/m²; P=.001). Methane-positive subjects also had a greater severity of constipation than methane-negative subjects (21.3±6.4 vs 9.5±2.4; P=.043). Multiple regression analysis illustrated a significant association between BMI and methane, constipation, and antidepressant use. However, methane remained an independent predictor of elevated BMI when controlling for antidepressant use (P<.001) and when controlling for both constipation and antidepressant use (6.55 kg/m² greater BMI; P=.003).
This is the first human study to demonstrate that a higher concentration of methane detected by breath testing is a predictor of significantly greater obesity in overweight subjects.

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Available from: Edy Soffer, Oct 06, 2014
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    • "Subjects with a breath CH 4 concentration above 1 ppmv of the atmospheric value were denoted as breath CH 4 producers (Bond et al 1971) or high emitters. As the laboratory air had a CH 4 concentration ratio of around 2 ± 0.1 ppmv the threshold was set to 3 ppmv, a value which is in agreement with the majority of previous studies (e.g.Basseri et al 2012). "
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    • "Undoubtedly, stressful life events have also been associated with an increased frequency of functional constipation [129]; however, this does not remove microbes from playing an important role in the modulation of gut motility and any potential systemic health consequences of chronic constipation. As discussed later, methane-producing intestinal microbes have been linked with constipation and are now emerging as a key part of reflex contraction and altered motility in IBS [130-133]. As we will discuss in Part III, beneficial microbes have recently been shown to reduce physio-behavioral signs associated with colorectal distention. "
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