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Polysaccharide and extracts from Lentinula edodes: Structural features and antiviral activity

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Lentinula edodes, known as shiitake, has been utilized as food, as well as, in popular medicine, moreover, compounds isolated from its mycelium and fruiting body have shown several therapeutic properties. The aim of this study was to determine the antiviral activity of aqueous (AqE) and ethanol (EtOHE) extracts and polysaccharide (LeP) from Lentinula edodes in the replication of poliovirus type 1 (PV-1) and bovine herpes virus type 1 (BoHV-1). The time-of-addition assay was performed at the times -2, -1, 0, 1 and 2 h of the infection. The virucidal activity and the inhibition of viral adsorption were also evaluated. Plaque assay was used to monitor antiviral activity throughout. The AqE and LeP were more effective when added at 0 h of infection, however, EtOHE was more effective at the times 1 h and 2 h of the infection. AqE, EtOHE and LeP showed low virucidal activity, and the inhibition of viral adsorption was not significant. The results allowed us to conclude that AqE, EtOHE and LeP act on the initial processes of the replication of both strains of virus.
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... In a study, anti-PV experiments were carried out on aqueous extract AqE, ethanol extract EtOHE and lentinan LeP of Lentinus edodes. The results showed that the three extracts showed antiviral activity and mainly played an inhibitory role in the early stage of virus infection [80]. ...
... Anti-BoHV-1 experiments were carried out on aqueous extract AqE, ethanol extract EtOHE and lentinan LeP of Lentinus edodes. The results showed that the three extracts showed antiviral activity and mainly played an inhibitory role in the early stage of virus infection [80]. ...
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... Given this fact, it is true that mushroom polysaccharides vary in their chemical composition, structure and anticancer activity [69,70]. The anticancer, antioxidative, immunomodulating activities of Ganoderma lucidum [70] fruiting bodies, which contain the heteroglycan Glycopeptide, have been reported; and the anticancer and immunomodulating properties of Lentinus edodes [69] fruiting bodies, which contain glucan, are well established. Pleurotus tuber-regium [71] fruiting bodies contain β-D-glucan, which displays anticancer as well as hepatoprotective properties. ...
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... It was reported that both aqueous and ethanol extracts of L. edodes showed a high et al. Bioresources and Bioprocessing (2022) 9:99 antiviral activity on the replication of poliovirus type 1 and bovine herpesvirus type 1 (Rincão et al. 2012). Also, lentinan was purified and showed antiviral activity from shiitake Lentinula edodes, which can suppress the surface expression of HIV (Seo and Choi 2021). ...
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... Kg (w\w) and 113-194 g respectively, but lowest from [2], who reported that total yield for three flushes was 233g. BE result agreed with [25]they reported that BE% was 25.12-54.17 %. ...
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... It was successfully applied for inoperable gastric cancer treatment in combination with some chemotherapies [155], but later was withdrawn due to skin-related side effects. L. edodes also showed antiviral activity [156] and immune support [157], as well as improvement of quality of life being observed when it was applied complementarily to other cancer immunotherapies [158]. ...
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