Silibinin Regulates Gene Expression, Production and Secretion of Mucin from Cultured Airway Epithelial Cells

Department of Thoracic Surgery, College of Medicine, Eulji University, Daejeon, Korea
Phytotherapy Research (Impact Factor: 2.66). 09/2012; 26(9):1301-7. DOI: 10.1002/ptr.3727
Source: PubMed


We investigated whether silibinin significantly affects gene expression, production and secretion of mucin from cultured airway epithelial cells. Confluent NCI-H292 cells were pretreated with silibinin for 30 min and then stimulated with epidermal growth factor (EGF), phorbol 12-myristate 13-acetate (PMA) or TNF-α for 24 h. The MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The effect of silibinin on TNF-α-induced activation of NF-κB p65 was also examined. Confluent primary rat tracheal surface epithelial (RTSE) cells were pretreated with adenosine triphosphate (ATP) for 5 min and then treated for 30 min in the presence of silibinin to assess the effect on mucin secretion using ELISA. The results were as follows: (i) silibinin inhibited the expression of the MUC5AC mucin gene induced by EGF, PMA or TNF-α from NCI-H292 cells; (ii) silibinin also inhibited the production of MUC5AC mucin protein induced by the same inducers from NCI-H292 cells; (iii) silibinin inhibited the activation of NF-κB p65 by TNF-α in NCI-H292 cells; (iv) silibinin significantly decreased ATP-induced mucin secretion from cultured RTSE cells. This result suggests that silibinin can regulate gene expression, production and secretion of mucin by directly acting on airway epithelial cells. Copyright © 2012 John Wiley & Sons, Ltd.

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    • "Silibinin attenuated EGF, PMA or TNF-a induced expression of the MUC5AC at the transcript and protein levels in NCI- H292 cells. Further, silibinin resulted in a significant reduction of ATP-induced mucin secretion in cultured rat tracheal surface epithelial (RTSE) cells [121]. Transcription factor Cdx2, has been shown to directly bind MUC2 and MUC4 promoters and upregulate M.A. Macha et al. / Cancer Treatment Reviews xxx (2015) xxx–xxx 7 Please cite this article in press as: Macha MA et al. "
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