A Role for Metaphase Spindle Elongation Forces in Correction of Merotelic Kinetochore Attachments

Department of Microbiology and Physiological Systems and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Current biology: CB (Impact Factor: 9.57). 02/2012; 22(3):225-30. DOI: 10.1016/j.cub.2011.12.022
Source: PubMed


During mitosis, equal segregation of chromosomes depends on proper kinetochore-microtubule attachments. Merotelic kinetochore orientation, in which a single kinetochore binds microtubules from both spindle poles [1], is a major cause of chromosome instability [2], which is commonly observed in solid tumors [3, 4]. Using the fission yeast Schizosaccharomyces pombe, we show that a proper force balance between kinesin motors on interpolar spindle microtubules is critical for correcting merotelic attachments. Inhibition of the plus-end-directed spindle elongation motors kinesin-5 (Cut7) and kinesin-6 (Klp9) reduces spindle length, tension at kinetochores, and the frequency of merotelic attachments. In contrast, merotely is increased by deletion of the minus-end-directed kinesin-14 (Klp2) or overexpression of Klp9. Also, Cdk1 regulates spindle elongation forces to promote merotelic correction by phosphorylating and inhibiting Klp9. The role of spindle elongation motors in merotelic correction is conserved, because partial inhibition of the human kinesin-5 homolog Eg5 using the drug monastrol reduces spindle length and lagging chromosome frequency in both normal (RPE-1) and tumor (CaCo-2) cells. These findings reveal unexpected links between spindle forces and correction of merotelic attachments and show that pharmacological manipulation of spindle elongation forces might be used to reduce chromosome instability in cancer cells.

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    • "That the spindle remains shorter than the cell in the oocyte is important, as an excessively long spindle would presumably preclude asymmetric cell divisions at MI and MII. Moreover, spindle length determination is important as defects in mechanisms that regulate spindle length are known to promote chromosome segregation errors in some systems[53]. Kinesin-5 has emerged as the key player in determining spindle length in mammalian oocytes. "
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