Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

Integrated Biosystems Lab, Intel Labs, Intel Corporation, 2200 Mission College Blvd., Santa Clara, CA 95054, USA.
The Analyst (Impact Factor: 4.11). 03/2012; 137(6):1351-62. DOI: 10.1039/c2an15930a
Source: PubMed


We introduce a label-free approach for sensing polymerase reactions on deoxyribonucleic acid (DNA) using a chelator-modified silicon-on-insulator field-effect transistor (SOI-FET) that exhibits selective and reversible electrical response to pyrophosphate anions. The chemical modification of the sensor surface was designed to include rolling-circle amplification (RCA) DNA colonies for locally enhanced pyrophosphate (PPi) signal generation and sensors with immobilized chelators for capture and surface-sensitive detection of diffusible reaction by-products. While detecting arrays of enzymatic base incorporation reactions is typically accomplished using optical fluorescence or chemiluminescence techniques, our results suggest that it is possible to develop scalable and portable PPi-specific sensors and platforms for broad biomedical applications such as DNA sequencing and microbe detection using surface-sensitive electrical readout techniques.

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Available from: David J Liu, Jan 02, 2014
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