Article

Antioxidant, Antimicrobial, and Anticancer Activity of 3 Umbilicaria Species

Department of Biology, Faculty of Science, University of Kragujevac, Kragujevac, Serbia.
Journal of Food Science (Impact Factor: 1.7). 01/2012; 77(1):T20-5. DOI: 10.1111/j.1750-3841.2011.02459.x
Source: PubMed

ABSTRACT

The aim of this study is to investigate in vitro antioxidant, antimicrobial, and anticancer activity of the acetone extracts of the lichens Umbilicaria crustulosa, U. cylindrica, and U. polyphylla. Antioxidant activity was evaluated by 5 separate methods: free radical scavenging, superoxide anion radical scavenging, reducing power, determination of total phenolic compounds, and determination of total flavonoid content. Of the lichens tested, U. polyphylla had largest free radical scavenging activity (72.79% inhibition at a concentration of 1 mg/mL), which was similar as standard antioxidants in the same concentration. Moreover, the tested extracts had effective reducing power and superoxide anion radical scavenging. Total content of phenol and flavonoid in extracts was determined as pyrocatechol equivalent, and as rutin equivalent, respectively. The strong relationships between total phenolic and flavonoid contents and the antioxidant effect of tested extracts were observed. The antimicrobial activity was estimated by determination of the minimal inhibitory concentration by the broth microdilution method. The most active was extract of U. polyphylla with minimum inhibitory concentration values ranging from 1.56 to 12.5 mg/mL. Anticancer activity was tested against FemX (human melanoma) and LS174 (human colon carcinoma) cell lines using MTT method. All extracts were found to be strong anticancer activity toward both cell lines with IC₅₀ values ranging from 28.45 to 97.82 μg/mL. The present study shows that tested lichen extracts demonstrated a strong antioxidant, antimicrobial, and anticancer effects. That suggests that lichens may be used as possible natural antioxidant, antimicrobial, and anticancer agents.

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Available from: Branislav R Ranković, Jan 28, 2014
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    • "This observation is in accordance with other studies (Yang and Anderson, 1999;Kosanić et al., 2012b;Ličina et al., 2013) focused on antimicrobial activity, which have demonstrated that bacteria are more sensitive to the antimicrobial activity than the fungi due to differences in the composition and permeability of the cell wall. The cell wall of Gram-positive bacteria is made of peptidoglucanes and teichoic acids, while the cell wall of Gram-negative bacteria is made of peptidoglucanes, lipopolysaccharides and lipoproteins (Heijenoort, 2001;Kosanić et al., 2012b). The cell wall of fungi is poorly permeable and consists of polysaccharides such as hitchin and glucan (Farkaš, 2003). "
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