Cloning, soluble expression, rapid purification and characterization of human Cofilin1

Biomedicine Research and Development Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, China.
Protein Expression and Purification (Impact Factor: 1.7). 03/2012; 82(1):186-91. DOI: 10.1016/j.pep.2012.01.002
Source: PubMed


Cofilin1 is an actin-binding protein that plays a critical role in the regulation of actin cytoskeleton and consequently affects various physiological processes. In this study, the human Cofilin1 cDNA was cloned into the expression vector pET-28a(+) with a 6 × His tag and expressed as soluble protein in Escherichia coli BL21(DE3). Approximately 78 mg of Cofilin1, which showed high activity as determined by native PAGE, could be purified from each liter of LB medium by His-tag affinity chromatography and gel filtration. Further, high-titer IgG against Cofilin1 was positively detected after immunization in rabbits and the polyclonal antibodies were purified and identified. Together, this report provides the first protocol to efficiently obtain human Cofilin1 with high biological activity and immunogenicity using E. coli BL21 (DE3) expression system.

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    • "Protein was expressed and purified as described previously. Briefly, TaADF7 in pET-41a (+) was expressed in E. coli strain BL21, and after induction with isopropyl-b-D-thiogalactoside, protein was extracted and purified as described previously (Moseley et al., 2006; Lu et al., 2012). Protein was pooled and dialyzed into KMEI buffer (Mason et al., 2011). "
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    • "Cells were lysed in RIPA buffer (Beyotime, P0013B) containing 1% PMSF (Beyotime, ST506) and clarified by centrifugation at 15,000Âg for 15 min at 4 °C. The protein concentration in the supernatant was measured using the BCA protein assay kit (Beyotime , P0010) [17]. The cell lysates were mixed with 5Â SDS–PAGE buffer (Beyotime, Jiangsu, China) and boiled for 5 min. "
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