Whole-Mount Confocal Microscopy for Vascular Branching Morphogenesis

ArticleinMethods in molecular biology (Clifton, N.J.) 843:69-78 · January 2012with15 Reads
Impact Factor: 1.29 · DOI: 10.1007/978-1-61779-523-7_7 · Source: PubMed

    Abstract

    We introduce a whole-mount immunohistochemistry method for analyzing intricate vascular network formation in mouse embryonic tissues. Laser scanning confocal microscopy with multiple labeling allows for robust imaging of blood and lymphatic vessel branching morphogenesis with excellent resolution.