Cytotoxic effect of fenitrothion and lambda-cyhalothrin mixture on lipid peroxidation and antioxidant defense system in rat kidney

Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt.
Journal of Environmental Science and Health Part B Pesticides Food Contaminants and Agricultural Wastes (Impact Factor: 1.2). 04/2012; 47(4):262-8. DOI: 10.1080/03601234.2012.636589
Source: PubMed


A mixture of pyrethroids plus organophosphates was assessed for their potential effects on lipid peroxidation, the antioxidant defense system and lactate dehydrogenase (LDH) in rat kidney in vitro. Various insecticide concentrations were incubated with kidney homogenate at 37°C for different incubation times. Treatment with fenitothion (FNT) plus lambda-cyhalothrin (LC) caused a significant induction (P < 0.05) in thiobarbituric acid reactive substances (TBARS), which might be associated to decreased levels of reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) activities and protein content in rat kidney. However, a significant induction of lactate dehydrogenase (LDH) activity was observed. The effect was concentration and time dependent. It can be concluded that depletion of GSH might indicate that reactive oxygen species (ROS) could be involved in the toxic effects of FNT plus LC which lead to marked perturbations in antioxidant defense system.

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Available from: Fatma El-Demerdash, Jul 22, 2014
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    • "Oxidative damage primarily occurs through production of reactive oxygen species (ROS) and can damage lipids, proteins and DNA. Therefore, oxidative damage may contribute to loss of enzymatic activity and structural integrity of enzymes and activate inflammatory processes [30] [31]. The increase of TBARS and decreased GSH observed in serum following DZN exposure was probably ascribed to the excessive production of ROS, which could be related with hepatocyte enzyme leakage. "
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    • "Lipid peroxidation (LPO) has been suggested as one of the molecular mechanisms involved in carbamate-induced toxicity (Banerjee et al., 1999). Enzymatic antioxidants and glutathione metabolism-regulating enzymes may protect the cellular system against various deleterious effects of free radicals induced by pesticides (El-Sharkawy et al., 1994; Mansour and Mossa, 2009; El-Demerdash, 2012). Thus, the present study was conducted to investigate the oxidative stress, enzymes alteration and histopathological changes induced by the insecticide methomyl on CD-1 mice. "
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