Article

Exposure and Kinetics of Polycyclic Aromatic Hydrocarbons (PAHs) in Cigarette Smokers

Center for Tobacco Control Research and Education, University of California, San Francisco, California, USA.
Chemical Research in Toxicology (Impact Factor: 3.53). 03/2012; 25(4):952-64. DOI: 10.1021/tx300043k
Source: PubMed

ABSTRACT

Our study objectives were (1) to investigate the selectivity of polycyclic aromatic hydrocarbon (PAH) metabolites for tobacco smoke exposure and (2) to determine half-lives of PAH metabolites in smokers. There were 622 participants from the United States (US) and Poland, and of these, 70% were smokers. All subjects provided spot urine samples, and 125 smokers provided blood samples. Urinary PAH metabolite half-lives were determined in 8 smokers. In controlled hospital studies of 18 smokers, the associations between various measures of nicotine intake and urinary excretion of PAH metabolites were investigated. Plasma nicotine was measured by GC. LC-MS/MS was used to measure the plasma levels of cotinine and trans-3'-hydroxycotinine, and urine levels of nicotine and its metabolites, total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and PAH metabolites (2-naphthol, 1-, 2-, and 3-hydroxyfluorenes, 1-, 2-, 3-, and 4-hydroxyphenanthrenes, and 1-hydroxypyrene). Regardless of smoking status, PAH metabolite excretion was higher in Polish subjects than in US subjects (p-values <0.001). 1-Hydroxyfluorene exhibited the greatest difference between smokers and nonsmokers, with a 5-fold difference in Polish subjects and a 25-fold difference in US subjects, followed by 3- and 2-hydroxyfluorenes, 2-naphthol, and 1-hydroxypyrene. The differences for hydroxyphenanthrenes were small or nonsignificant. 1-Hydroxyfluorene had the highest correlation with urine nicotine equivalents (r = 0.77) and urine NNAL (r = 0.64). While the half-lives of PAH metabolites were <10 h in smokers, 1-hydroxyfluorene had the largest ratio of initial to terminal urine concentration (58.4 ± 38.6, mean ± SD) after smoking. Receiver Operating Characteristic (ROC) analysis of PAHs among Polish and US subjects further showed that hydroxyfluorenes are most highly discriminative of smokers from nonsmokers followed by 2-naphthol and 1-hydroxypyrene. In conclusion, hydroxyfluorenes, particularly 1-hydroxyfluorene, and 2-naphthol are more selective of tobacco smoke than 1-hydroxypyrene and hydroxyphenanthrenes. Characterization of hydroxyfluorene and 2-naphthol metabolites in urine may improve the characterization of PAHs from tobacco smoke and related disease risks among smokers and nonsmokers.

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Available from: Gideon St.Helen, Feb 13, 2015
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    • "They are generated during the incomplete combustion of fossil fuel, oil refinement, and industrial and municipal discharges. Also, tobacco and barbequed or smoked meat are potential matrices to find the PAHs [1] [2]. PAHs have attracted attention of biochemists and analytical chemists because of their carcinogenicity, mutagenicity , toxicity and environmental persistence. "
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    ABSTRACT: In the present research, ultrasonic-assisted emulsification-microextraction (USAEME) coupled with gas chromatography-mass spectrometry (GC-MS) has been proposed for analysis of thirteen environmental protection agency (EPA) polycyclic aromatic hydrocarbons (PAHs) in aqueous samples. Tetrachloroethylene was selected as extraction solvent. The main parameters of USAEME affecting the efficiency of the method were modeled and optimized using a central composite design (CCD). Under the optimum conditions (9μL for extraction solvent, 1.15% (w/v) NaCl (salt concentration) and 10min for ultrasonication time), preconcentration factor (PF) of the PAHs was in the range of 500-950. In order to have a comprehensive analysis, multivariate curve resolution-alternating least squares (MCR-ALS) as a second-order calibration algorithm was used for resolution, identification and quantification of the target PAHs in the presence of uncalibrated interferences. The regression coefficients and relative errors (REs, %) of calibration curves of the PAHs were in the satisfactory range of 0.9971-0.9999 and 1.17-6.59%, respectively. Furthermore, analytical figures of merit (AFOM) for univariate and second-order calibrations were obtained and compared. As an instance, the limit of detections (LODs) of target PAHs were in the range of 1.87-18.9 and 0.89-6.49ngmL(-1) for univariate and second-order calibration, respectively. Finally, the proposed strategy was used for determination of target PAHs in real water samples (tap and hookah waters). The relative recoveries (RR) and the relative standard deviations (RSDs) were 68.4-109.80% and 2.15-6.93%, respectively. It was concluded that combination of multivariate chemometric methods with USAEME-GC-MS can be considered as a new insight for the analysis of target analytes in complex sample matrices. Copyright © 2015 Elsevier B.V. All rights reserved.
    Full-text · Article · Aug 2015 · Journal of Chromatography A
    • "These current results are explained by variable PAHs aerosol combined with respiratory uptake of gPyr through P3 respirators , and also by an important cutaneous absorption of particulate PAHs which can penetrate into the organism via unprotected wrist or neck, but also through skin areas covered by contaminated work clothes (Van Rooij et al. 1992). Indeed, it is well established that PAHs absorption process strongly influences the kinetics of urinary excretion of the various metabolites (Lafontaine et al. 2002;Li et al. 2012;Payan et al. 2009;Sobus et al. 2009b;St Helen et al. 2012;). Urinary levels of the two metabolites showed no progressive increase over the working week, except for W5 and W6, contrary to what was previously reported for 1-OHP in carbon electrode production (Petry et al. 1996). "
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    No preview · Article · Mar 2015 · International Archives of Occupational and Environmental Health
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    • "The general population is exposed to PAHs from the environment through small-sized respirable particles, water and food, as well as from the occupational environment (6). The most widespread manner of PAH input is the inhalation of exhaust gases, tobacco smoke, and the usage of products and materials containing PAHs (7). Contrary to popular opinion, alimentary exposure causes a higher rate of human exposure to PAHs than air pollutants do (8). "
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