Engineering genes for predictable protein expression

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Protein Expression and Purification (Impact Factor: 1.7). 03/2012; 83(1):37-46. DOI: 10.1016/j.pep.2012.02.013
Source: PubMed


The DNA sequence used to encode a polypeptide can have dramatic effects on its expression. Lack of readily available tools has until recently inhibited meaningful experimental investigation of this phenomenon. Advances in synthetic biology and the application of modern engineering approaches now provide the tools for systematic analysis of the sequence variables affecting heterologous expression of recombinant proteins. We here discuss how these new tools are being applied and how they circumvent the constraints of previous approaches, highlighting some of the surprising and promising results emerging from the developing field of gene engineering.

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Available from: Sridhar Govindarajan
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    • "Given a target protein, its coding sequence can affect both transcription and translation processes [15, 88]. As described above, mRNA secondary structures could affect mRNA degradation and limit RBS accessibility to ribosomes and, in addition, AT-rich sequences can cause premature transcriptional termination [89]. Codon usage has been reported to affect the translation process [90]. "
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