Effects of low-level laser therapy (GaAs) in an animal model of muscular damage induced by trauma

Laboratório de Fisiologia e Bioquímica do Exercício, Universidade do Extremo Sul Catarinense, Av. Universitária, 1105-Bairro Universitário, 88806-000, Criciúma, SC, Brazil, .
Lasers in Medical Science (Impact Factor: 2.49). 03/2012; 28(2). DOI: 10.1007/s10103-012-1075-6
Source: PubMed


It has been demonstrated that reactive oxygen species (ROS) formation and oxidative damage markers are increased after muscle damage. Recent studies have demonstrated that low-level laser therapy (LLLT) modulates many biochemical processes mainly those related to reduction of muscular injures, increment of mitochondrial respiration and ATP synthesis, as well as acceleration of the healing process. The objective of the present investigation was to verify the influence of LLLT in some parameters of muscular injury, oxidative damage, antioxidant activity, and synthesis of collagen after traumatic muscular injury. Adult male Wistar rats were divided randomly into three groups (n = 6), namely, sham (uninjured muscle), muscle injury without treatment, and muscle injury with LLLT (GaAs, 904 nm). Each treated point received 5 J/cm(2) or 0.5 J of energy density (12.5 s) and 2.5 J per treatment (five regions). LLLT was administered 2, 12, 24, 48, 72, 96, and 120 h after muscle trauma. The serum creatine kinase activity was used as an index of skeletal muscle injury. Superoxide anion, thiobarbituric acid reactive substance (TBARS) measurement, and superoxide dismutase (SOD) activity were used as indicators of oxidative stress. In order to assess the synthesis of collagen, levels of hydroxyproline were measured. Our results have shown that the model of traumatic injury induces a significant increase in serum creatine kinase activity, hydroxyproline content, superoxide anion production, TBARS level, and activity of SOD compared to control. LLLT accelerated the muscular healing by significantly decreasing superoxide anion production, TBARS levels, the activity of SOD, and hydroxyproline content. The data strongly indicate that increased ROS production and augmented collagen synthesis are elicited by traumatic muscular injury, effects that were significantly decreased by LLLT.

Download full-text


Available from: Débora L Scheffer, Jun 12, 2014
  • Source
    • "For example, LLLT/PBM has been shown to attenuate the production of ROS by human neutrophils (Fujimaki et al, 2003). Silveira et al (2013) reported that LLLT/PBM reduced ROS in an animal model of traumatic tissue injury; whereas a study in a model of acute lung inflammation found LLLT/PBM to reduce the generation of TNF-α and to increase IL-10, an anti-inflammatory cytokine (de Lima et al, 2011). In addition, NO is a potent vasodilator (Lohr et al, 2009) and can increase the blood-supply to the illuminated tissue. "

    Full-text · Dataset · Dec 2015
  • Source
    • "The accumulation of ROS, as well as an increase in oxidative stress, has been well established by our group in muscular injury by trauma (Silveira et al. 2012, 2013; Victor et al. 2012) or by physical exercise (Scheffer et al. 2012; Silva et al. 2011, 2013). High levels of ROS are constantly generated and cause oxidative damage to nucleic acids, proteins and lipids when the anti-oxidant and repair systems are ineffective or insufficient. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of the study described here was to investigate the effects of pulsed ultrasound and gold nanoparticles (AuNPs) on behavioral, inflammatory and oxidative stress parameters in an experimental model of overuse. Wistar rats performed 21 d of exercise on a treadmill at different intensities and were exposed to ultrasound in the presence or absence of AuNPs. The overuse model promoted behavioral changes and increased creatine kinase, superoxide dismutase and glutathione peroxidase activity, as well as the levels of superoxide, nitrotyrosine, nitric oxide, thiobarbituric acid reactive substance, carbonyl, tumor necrosis factor α and interleukin-6. These values were significantly decreased by AuNPs and by AuNPs plus ultrasound. Catalase activity remained unchanged and the glutathione level increased significantly after exposure to AuNPs plus ultrasound. These results suggest a susceptibility to anxiety as well as elevated levels of oxidative stress. However, therapeutic interventions with AuNPs plus ultrasound reduced the production of oxidants and oxidative damage and improved the anti-oxidant defense system.
    Full-text · Article · Nov 2014 · Ultrasound in Medicine & Biology
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The use of low level laser (light) therapy (LLLT) has recently expanded to cover areas of medicine that were not previously thought of as the usual applications such as wound healing and inflammatory orthopedic conditions. One of these novel application areas is LLLT for muscle fatigue and muscle injury. Since it is becoming agreed that mitochondria are the principal photoacceptors present inside cells, and it is known that muscle cells are exceptionally rich in mitochondria, this suggests that LLLT should be highly beneficial in muscle injuries. The ability of LLLT to stimulate stem cells and progenitor cells means that muscle satellite cells may respond well to LLLT and help muscle repair. Furthermore the ability of LLLT to reduce inflammation and lessen oxidative stress is also beneficial in cases of muscle fatigue and injury. This review covers the literature relating to LLLT and muscles in both preclinical animal experiments and human clinical studies. Athletes, people with injured muscles, and patients with Duchenne muscular dystrophy may all benefit.
    Full-text · Article · Nov 2012
Show more