ELISA. A representative experiment of five is shown. n.d. not
detectable. * p-value,0.05 versus control mice.
Figure S3 Impaired Th1 cell development in
mice is not dependent on the L. major
parasite strain. N1N2
and control N1N2
were infected s.c. with 3610
L. major promastigotes of L. major
Friedlin (A) or IR75 (B) and LV39 (C) strains. 6 weeks after
T cells were isolated, restimulated or not with
UV-irradiated L. major Friedlin (A), IR75 (B) or LV39 (C) in
presence of irradiated spleen cells, and 72 hours later, IFNc and
IL-13 secretion was quantified in supernatants. Mean cytokine
secretion 6 SEM are given (n$3 mice per group). (D) The
intralesional parasite load was assessed by LDA 6 weeks post
infection with L. major Friedlin and IR75. Mean number of
parasite per lesion is represented 6 SEM for n$3 mice per group.
Data are representative of at least 3 individual experiments. * p-
value,0.05 versus control mice.
We thank, Dr Markus Mohrs for advice with the STAT1 assay and
discussions, Dr P. Branigan (Centocor) for the anti-IL-13 mAb and
discussions, Gre´goire Simon for analysis of alternate macrophage
activation, Dr Emma Fiorini and Anne Wilson for discussion, Dr Esther
von Stebut for the L. major (Friedlin), Yazmin Hauyon-La Torre, and
Tatiana Proust for technical expertise, Danny Labes for Cell sorting.
Conceived and designed the experiments: FTC. Performed the experi-
ments: FA SS MC FD EM. Analyzed the data: FA FTC. Contributed
reagents/materials/analysis tools: UK FR HRM. Wrote the paper: FTC.
Contributed to the design of the experiments: FA. Contributed to the
editing of the manuscript: FA HRM FR.
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PLoS Pathogens | www.plospathogens.org 10 March 2012 | Volume 8 | Issue 3 | e1002560