Antidiarrhoeal Activity of Leaf Extract of Moringa Oleifera in Experimentally Induced Diarrhoea in Rats

International Journal of Phytomedicine 01/2011;


To evalauate the antidiarrhoeal activ ity of the hydroalcoholic extract of
moringa oleifera leaves. The hydroalc hoholic extract was evaluated using
rodent animal m odels of diarrhoea like the castor oil and ma gnesium sulfate
induced gastrointestinal motility, in a model of enteropoo ling induced by the
admi nistration of castor oil and PGE2, Charcoal meal test. Acute toxi city
and phytochemi cal constituents were also been evaluated using standardized
methods. The results of the present study indicates that the hydroalcoholic
extract of moringa oleifera leaves wa s effective in inducing a significant
protection against experime ntally induced diarrhoea by castor oil and
magnesium sulfate, as evidenced by a decrease in the numb er of frequency,
weight of stools after 4 hours with respect to control. The extract also
prevented the enteropooling induced by cas tor oil and PGE2 at all the doses
tested. Acute toxicity s tudies indic ated that the extract is saf e till 2500
mg/kg. The antidiarrhoeal activity though, not ascribed to any particular
phytochemi cal present, general tests performed indicated the presence of
flavonoids, tannis which were reported to produce antidiarrhoeal activity.
These results showed that Moringa ol eifera leaves posse ss anti-diarrheal
properties mediated through inhibition of hyper secretion and
gastrointe stinal mo tility that substantiate its use in the treatm ent of diarrhea
in traditiona l me dicines or folklore us e.

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    • "It includes loss of electrolytes and water and decreased absorption of fluid from the gastrointestinal tract. It also involves increased frequency of bowel movements (Lakshminarayana et al., 2011). Diarrhea has long been recognized as one of the most important health problems and leading cause of mortality and morbidity in the developing countries (Rajamanickam et al., 2010) and produces more illness and causes death of more infants and children below 5 years old than all other diseases combined (Dalal et al., 2011). "
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    ABSTRACT: Zinc supplementation is a critical new intervention for treating diarrheal episodes in children. Recent studies suggest that administration of zinc along with new low osmolarity oral rehydration solutions / salts (ORS) can reduce the duration and severity of diarrheal episodes for up to three months. Several mechanisms of action of zinc has been proposed, however there is dearth of information about the effect of zinc on intestinal motility during diarrhea. Male albino Wistar rats (80-100g) were used. The effect of different doses of zinc sulphate (25, 50, 100, 150mg/Kg) on the number of wet faeces was investigated. Intestinal motility during castor oil induced diarrhea was assessed using activated charcoal meal and the mechanisms of action of zinc sulphate on motility were investigated. The effective dose of zinc sulphate (100mg/Kg) significantly reduced (p< 0.001) the number of wet faeces (3.0 ± 0.00) compared with control (6.8 ± 0.25) during diarrhea. This antidiarrheal effect of zinc was abolished by propranolol and nifedipine. Zinc sulphate significantly reduced (p< 0.05) intestinal transit time (60.7 ± 7.13%) compared with control (85.7 ± 2.35%). It is concluded that zinc sulphate reduces the frequency of wet faeces output and intestinal motility during diarrhea via activation of β adrenergic receptor and L-type Ca2+ channel.
    Full-text · Article · Jul 2015 · Nigerian journal of physiological sciences: official publication of the Physiological Society of Nigeria

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    ABSTRACT: Diarrhoea is a common disease which causes pain and may be deadly, especially in developing countries. In Bangladesh, diarrhoeal diseases affect thousands of people every year, and children are especially vulnerable. Bacterial toxins or viral infections are the most common cause of the disease. The diarrhoea outbreaks are often associated with flood affected areas with contaminated drinking water and an increased risk of spreading the water-borne disease. Not surprisingly, plants found in the near surroundings have been taken into use by the local community as medicine to treat diarrhoeal symptoms. These plants are cheaper and more easily available than conventional medicine. Our question is: What is the level of documentation supporting the use of these plants against diarrhoea and is their consumption safe? Do any of these plants have potential for further exploration? In this review, we have choosen seven plant species that are used in the treatment of diarrhoea; Diospyros peregrina, Heritiera littoralis, Ixora coccinea, Pongamia pinnata, Rhizophora mucronata, Xylocarpus granatum, and Xylocarpus moluccensis. Appearance and geographical distribution, traditional uses, chemical composition, and biological studies related to antidiarrhoeal activity will be presented. This review reveals that there is limited scientific evidence supporting the traditional use of these plants. Most promising are the barks from D. peregrina, X. granatum and X. moluccensis which contain tannins and have shown promising results in antidiarrhoeal mice models. The leaves of P. pinnata also show potential. We suggest these plants should be exploited further as possible traditional herbal remedies against diarrhoea including studies on efficacy, optimal dosage and safety.
    Full-text · Article · May 2013 · Nutrients
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