Conference Paper

Effect of the Inonotus Obliquus Polysaccharides on Blood Lipid Metabolism and Oxidative Stress of Rats Fed High-Fat Diet In Vivo

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Abstract

Inonotus Obliquus is a medicinal mushroom that is widely used in folk medicine in Russia, North Europe and China. The objective of this study was to evaluate the antioxidant and hypolipidemic capacities of Inonotus Obliquus polysaccharides(IOP). In vivo, the hyperlipidemic rats, induced by high-fat diet, were provided 100, 200, 500 mg/kg day of IOP for 8 weeks to evaluate their expected hypolipidemic activity. The results showed that Compared with MG, The administration of lOP did not show any effect on the body weight of the experimental rats, but significantly decreased the levels of LDL-C, TC, TG in serum, increased the activities of SOD and GSH-Px(P

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... Diao et al. (2014) reported that crude polysaccharides from I. obliquus could ameliorate the diabetic symptoms induced by streptozotocin. Liang et al. (2009) reported that I. obliquus polysaccharides showed great antioxidant and hypolipidemic capacities in hyperlipidemic rats. The hot water extractions of I. obliquus were also reported to be beneficial in preventing obesity and related metabolic abnormalities in C57BL/6J mice (Soh et al., 2008). ...
... C. Wang et al. / Food and Chemical Toxicology xxx (2017) Currently, numerous studies have proved that polysaccharides exhibit anti-diabetic activity both in vitro and in vivo (Wu et al., 2016). Several studies have reported the anti-diabetic potential of polysaccharides from I. obliquus (Diao et al., 2014;Liang et al., 2009;Soh et al., 2008). Trivalent chromium, one of the most common elements, is an essential nutrient with very low toxicity. ...
... 7 Some studies have illustrated that crude polysaccharides from I. obliquus ameliorate diabetic symptoms and that they show great antioxidant and hypolipidemic capacities. 8,9 A triterpene called inotodiol has antiproliferative properties, demonstrated in vitro with human lung adenocarcinoma (A549), cancer-derived, and HeLa cells. 10 Genome sequencing has become more rapid and more practical, but genome sequences alone are insufficient to explain biological characteristics. ...
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Inonotus obliquus is a rare, edible and medicinal fungus that is widely used as a remedy for various diseases. Its main bioactive substances are polysaccharides and terpenoids. In this study, we characterized and investigated the pan-genome of three strains of I. obliquus. The genome sizes of JL01, HE, and NBRC8681 were 32.04, 29.04, and 31.78 Mb, respectively. There were 6 543 core gene families and 6 197 accessory gene families among the three strains, with 14 polysaccharide-related core gene families and seven accessory gene families. For terpenoids, there were 13 core gene families and 17 accessory gene families. Pan-genome sequencing of I. obliquus has improved our understanding of biological characteristics related to the biosynthesis of polysaccharides and terpenoids at the molecular level, which in turn will enable us to increase the production of polysaccharides and terpenoids by this mushroom.
... IO possesses various pharmacological and biological properties, including antitumor, antimicrobial and antioxidant activities (19). It was previously reported that IO inhibited lipid peroxidation in hyperlipidemic rats (20). In the present study, IOE exerted cardioprotective effects by attenuating oxidative damage and suppressing ER stress-induced apoptosis. ...
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Inonotus obliquus (IO) is an edible fungus that exerts various biological functions, including anti‑inflammatory, antitumor and immunomodulatory effects. The present study was designed to investigate the role of IO extract (IOE) in myocardial ischemia/reperfusion (MI/R) and determine the exact molecular mechanisms. The left anterior descending coronary artery was ligated to establish the MI/R injury model in rats. IOE exhibited a novel cardioprotective effect, as shown by improvement in cardiac function and decrease in infarct size. Pretreatment with IOE activated antioxidant enzymes in cardiomyocytes, including glutathione peroxidase, superoxide dismutase and catalase. IOE pretreatment also induced the upregulation of NAD‑dependent protein deacetylase sirtuin‑1 (SIRT1) and downregulation of glucose‑regulated protein 78, phosphorylated (p‑) protein kinase R‑like endoplasmic reticulum kinase, p‑eukaryotic translation initiation factor 2 subunit α, C/EBP homologous protein and caspase‑12. Furthermore, IOE alleviated endoplasmic reticulum (ER) stress‑induced apoptosis in cardiomyocytes by decreasing the mRNA levels of caspase‑12. IOE inhibited apoptosis induced by overexpression of pro‑caspase‑9 and pro‑caspase‑3. In summary, IOE pretreatment protects the heart against MI/R injury through attenuating oxidative damage and suppressing ER stress‑induced apoptosis, which may be primarily due to SIRT1 activation.
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