Antimicrobial and Cytotoxic Activity of the Methanol Extract of Paederia foetida Linn. (Rubiaceae)

Journal of Applied Pharmaceutical Science (Impact Factor: 0.47). 01/2012; 02(01):77-80.


The antibacterial activities of n-hexane, chloroform, ethyl acetate fractions of
methanolic extracts of the whole plants Paederia foetida (family Rubiaceae) were screened
against various pathogenic bacteria such as Bacillus cereus, Bacillus megaterium, Bacillus
subtilis, Staphylococcus aureus, Sarcina lutea, Escherichia coli, Pseudomonas aeruginosa,
Salmonella paratyphi , Salmonella typhi, Shigella boydii, Shigella dysenteriae, Vibrio mimicus,
Vibrio parahemolyticus, Candida albicans, Aspergillus niger, Sacharomyces cerevacae by ‘disc
diffusion method’. The methanol extract of the whole plants possesses no antimicrobial activity
but the ethyl acetate, chloroform and n-hexane fractions exhibited moderate to less activity
against some organisms tested compared with the standard antibiotic Kanamycin. Brine shrimp
lethality bio-assay was done using brine shrimp Nauplii and dimethyl sulfoxide as a solvent for
the methanol plant extracts of Paederia foetida. The LC50 value of methanol extract of the plant
indicated that the cytotoxicity was very significant

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Available from: Maizbha Uddin Ahmed
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    ABSTRACT: OBJECTIVE: To evaluate in vitro antibacterial potentiality of 5 ethnomedicinal plants used by the Indian aborigine Kandha, against 8 multidrug resistant enteropathogenic bacteria isolated from clinical samples of under-5 hospitalized children. METHODS: Antibiotic sensitivity patterns of eight clinically isolated strains of enteropathogenic bacteria, Enterobacter aerogenes, Escherichia coli, K. pneumoniae, Salmonella paratyphi, Salmonella typhi, Shigella dysenteriae, Shigella sonnei and Vibrio cholerae were assessed by disc-diffusion method. Antibacterial activities of 8 solvent-extracts of leaves and bark of 5 medicinal plants were monitored by the agar-well diffusion method. Further, values of minimum inhibitory concentration and minimum bactericidal concentration were assessed by the microbroth dilution method. Qualitative phytochemical analyses of active plant extracts were carried out. RESULTS: Ethanol, ethyl acetate and methanol leaf-extracts of Holarrhena antidysenterica of eight solvent-extracts were highly effective against 8 MDR pathogens in vitro. Acetone, ethanol and methanol-leaf extracts of Terminalia alata, chloroform, ethyl acetate and methanol leaf-extracts of Terminalia arjuna, and ethyl acetate, ethanol and methanol extracts of Paederia foetida were highly effective in inhibiting in vitro growth of 8 MDR enteropathogens. In succinct, ethyl acetate and methanol bark-extracts of H. antidysenterica, acetone, ethanol and methanol bark-extracts of T. alata, and acetone, ethanol and methanol bark-extracts of T. arjuna were highly effective. MIC and MBC values of the best 3 active leaf and bark extracts for five plants were in the range 1.56 to 50 mg/mL. CONCLUSIONS: These 5 plants were recorded to have in vitro control over a cohort of 8 enteropathogenic bacterial strains isolated from clinical samples.
    Full-text · Article · Jan 2015 · Journal of integrative medicine