Solid-state fermentation for the production of alkaline protease by Bacillus cereus 1173900 using proteinaceous tannery solid waste

ArticleinCurrent science 100(5):726-730 · March 2011with90 Reads
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    Abstract

    Animal fleshing, the major proteinaceous solid waste
    generated from leather industry, was used as substrate
    for the production of alkaline protease by Bacillus
    cereus 1173900 in solid-state fermentation (SSF).
    Maximum protease activity of 12,310 U/g was observed
    at 60 h in SSF crude extract. The extracted
    protease enzyme was purified by 53.64-fold through
    ammonium sulphate precipitation and chromatography
    separation in Sephadex G-100. The purified protease
    had a specific activity of 201.6 (U/mg). The molecular
    weight of the purified enzyme was 66 kDa, determined
    by SDS–PAGE. The zymogram also revealed a clear
    hydrolytic zone due to proteolytic activity, which
    coincided with the band obtained with SDS–PAGE.
    Enzyme activity was inhibited by EDTA, suggesting
    that the enzyme belongs to metalloprotease(s).