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Comparison Between Alkaline and Neutral Variants of Yeast Comet Assay
Abstract and Figures
Comet assay gain its popularity because it is a fast and relatively easy-to-perform method for assessing damages in DNA. The two persisting variants of the method: alkaline and neutral, lead to some doubts which one would be suitable for a certain purpose. Here we present a comparison between the two versions of the developed by us Yeast Comet Assay (YCA). Since DNA damages can be quite diverse, i.e. single- or double- stranded cuts, oxidative damages, photodimers, etc., we warn that the method of YCA has to be applied with attention and a certain level of understanding concerning the exact damage that is attempted to be assessed.
Figures - uploaded by Ekaterina Peycheva
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