Water solubilization of hydrophobic nanocrystals by means of poly(maleic anhydride-alt-1-octadecene)

ArticleinJournal of Materials Chemistry 18(17):1991-1996 · January 2008with170 Reads
DOI: 10.1039/B717801H
Abstract
Poly(maleic anhydride-alt-1-octadecene), a cheap and commercially available polymer, was used to water-solubilize colloidal nanocrystals with various compositions, morphologies, and sizes. Highly pure nanoparticles with homogeneous distributions of sizes and surface charges were obtained after a single purification step of the polymer-coated particles by ultracentrifugation, saving precious time as compared to a previously published and similar polymer coating procedure. This simple strategy proved also to be generally applicable and represents a valid methodology to water-solubilize nanoparticles.
    • "After evaporation of the solvent, the hydrolysis of the maleic anhydride units of PMAO into carboxylic acids was conducted with NaOH under ultrasonication at 60 °C (the polymer will be noted PMO, thereafter). This elevated temperature and the extended (1 h) ultrasonication were crucial for successful water-dispersion, similarly to the synthetic route described by Di Corato [22] . Chloroform slowly evaporated during that step forcing QDs to transfer into the aqueous phase. "
    [Show abstract] [Hide abstract] ABSTRACT: In this paper, the synthesis of alloyed CuInZnxS2+x quantum dots (ZCIS QDs), their transfer into aqueous solution via a polymer coating technique, and the use of these nanocrystals to selectively target HER2-positive cells, are reported. By optimizing first the ZnS shell deposition process onto the CuInS2 core, and next the encapsulation of the dots with the amphiphilic poly(maleic anhydride-alt-1-octadecene) (PMAO) polymer, water-dispersible ZCIS QDs were successfully prepared. The nanocrystals with a photoluminescence quantum yield of 35% were purified via centrifugation and ultracentrifugation and high quality nanoparticles with narrow size distributions and surface charges were obtained. After verifying the biocompatibility of PMO-coated ZCIS QDs, we coupled these nanocrystals with the LTVSPWY peptide and demonstrated via MTT assay that both bare and the peptide-linked QDs exhibit low cytotoxicity. The HER2-mediated delivery of the peptide-linked QDs was confirmed by confocal microscopy. This study indicates that as engineered QDs can efficiently be used as fluorescent nanoprobes for selective labelling of HER2-positive SKBR3 cancer cells.
    Full-text · Article · Feb 2016
    • "Amphiphilic alternating copolymers such as poly (MA-alt-1- tetradecene) and poly (MA-alt-1-octadecene), and their poly (ethylene oxide) (PEO)-grafted derivatives have been used for the surface functionalization and encapsulation of magnetic nanoparticles262728. These copolymers, with longer hydrophobic branches and their amine cross-linked networks, provide a stable nanoparticle shell and hydrophobic double layer as well as water solubility of the nanoparticles [24, 26,27]. Maleic anhydride (MA) reactive copolymers, including poly(MA-alt-α-olefin)s are being widely used to prepare high performance engineering, bioengineering and nanoengineering materials28293031 . "
    [Show abstract] [Hide abstract] ABSTRACT: This work presents a new approach to fabrication and characterization of novel polymer nanofiber electrolytes from intercalated PVA/ODA-MMT nanocomposite as a matrix polymer and encapsulated graphene oxide (GO) nanosheets with amphiphilic reactive copolymer as partner polymers using electrospinning method. The chemical and physical structures, surface morphology, thermal behaviors and electric conductivity of nanocomposites and nanofibers were investigated using analyses methods including FTIR, XRD, SEM, DSC-TGA and conductivity analysis. Significant improvements in nanofiber morphology and size distribution were observed when GO and reactive organoclay were incorporated as reinforcement fillers into various matrix/partner solution blends. The structural factors of matrix-partner polymer nanocomposite particles with higher zeta-potential play important roles in both chemical and physical interfacial interactions and phase separation processing and also lead to the formation of nanofibers with unique surface morphologies and good conductivities. The cytotoxic, necrotic and apoptotic effects of chosen nanofibers on osteocarcinoma cells were also investigated. These multifunctional, self-assembled, nanofibrous surfaces can serve as semi-conductive and bioactive platforms in various electrochemical and bio-engineering processes, as well as reactive matrices used for the immobilization of various biopolymer precursors.
    Full-text · Article · Dec 2015
    • "After evaporation of the solvent, the hydrolysis of the maleic anhydride units of PMAO into carboxylic acids was conducted with NaOH under ultrasonication at 60 °C (the polymer will be noted PMO, thereafter). This elevated temperature and the extended (1 h) ultrasonication were crucial for successful water-dispersion, similarly to the synthetic route described by Di Corato [22] . Chloroform slowly evaporated during that step forcing QDs to transfer into the aqueous phase. "
    [Show description] [Hide description] DESCRIPTION: Fluorescence allows to stain at molecular level that we can track and unravel a wealth of information on cells, organs etc. That’s why the optical imaging is the rapidly growing field that provides information in basic research as well as in clinical applications [1]. The organic dyes, although routinely used in fluorescence imaging and spectroscopy, they possess some limitations, that make them often insufficient for the bioimaging purposes [2,3]. This became a motivation to seek new biomarkers. Consequently, quantum dots (QDs) due to their great intrinsic photophysical properties, quickly supplied that need, ultimately opening a new emerging field of studying QDs-based fluorescent nanoplatforms for biological, medical and fundamental studies applications. Here, we propose a new biocompatible polymer-coated CuInS2/ZnS QDs that possess high PL QY up to 35% and long colloidal stability. The nanocrystals were further functionalized with a HER2 receptor-binding peptide as a targeting ligand. The peptide-linked QDs suitability as a potential fluorescent nanoprobe as well as their specificity to HER2-positive cancer cells were investigated.
    Poster · Jun 2015 · Materials Science and Engineering C
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