Production of NO and N2O by Heterotrophic Nitrifier Alcaligenes faecalis Parafaecalis under Varying Conditions of Oxygen Saturation

Klinikum Garmisch-Partenkirchen, Markt Garmisch-Partenkirchen, Bavaria, Germany
Geomicrobiology (Impact Factor: 1.44). 07/2006; 23:165-176. DOI: 10.1080/01490450600599221


Production of NO and N2O by the heterotrophic nitrifier Alcaligenes faecalis subsp. parafaecalis was studied during growth in batch and continuous culture on peptone-meat extract medium. Depending on oxygen saturation level, medium redox status and amount of substrate supplied, the microorganisms produced 0.002–0.25 mg NO-N h (g protein) and 0.16–2.4 mg N2O-N h (g protein). Maximum rates of nitrogen oxides production were observed during peak events initiated by sudden changes of oxygen supply in the medium and were due to combined nitrification/denitrification taking place simultaneously within the cells. Based on model simulations of enzymatic kinetics of denitrification, possible mechanisms of increased nitrogen oxides production during periods of changes in oxygen supply are suggested.

Download full-text


Available from: Sergey Blagodatsky
  • Source
    • "The heterotrophic bacteria that have the ability for nitrification are Pseudomonas, Alcaligenes faecalis, and Comamonas sp. [12] [14]. "

    Full-text · Dataset · Sep 2015
  • Source
    • "Alcaligenes faecalis and Comamonas sp. have the ability for HN [18] [20]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Abstract A comparative optimization study using response surface methodology (RSM) was carried out for biological removal of various inorganic N-NH3 sources (NH4OH, (NH4)2SO4, NH4NO3) along with chemical oxygen demand (COD) (glucose: C6H12O6) from synthetic wastewater and consequently minimizing the N-N2O emission. Heterotrophic biomass conversion (HBC) process was used for wastewater treatment. Central composite design (CCD) method was used to optimize various experimental parameters using RSM. During the experiment biomass (total volatile suspended solids) was kept constant, while varying nitrogen and carbon concentration, respectively. Alkalinity consumption results in pH decrease during the HBC process. N-NH3 and COD depletion rates were higher in NH4OH in comparison to the other two nitrogen sources. Analyses of variance (ANOVA) observed highly significant differences. Models obtained through CCD method were checked and tested via experiments and statistics. Having optimum value, i.e. 210 mg/L N-NH3, 2.90 g/L COD and 38 h; higher COD (94.1%) and N-NH3 (82.72%) removal was observed in (NH4)2SO4 and NH4OH. Higher desirability factor was obtained in NH4OH.
    Full-text · Article · Nov 2014 · CLEAN - Soil Air Water
  • Source
    • "Under oxygen limitation , nitrate serves as electron acceptor for denitrification in the glucose plus nitrate treatment. When nitrate is limited but N is present as glycine (C/N ratio of 2), both heterotrophic nitrification and denitrification may occur in same microbial cell (Blagodatsky et al. 2006). "
    [Show abstract] [Hide abstract]
    ABSTRACT: We investigated how oxygen availability, substrate amount and quality affect the temperature dependency of enzymatic processes involved in the production of CO2 and N2O. Three substrates differing in microbial degradability (glucose with potassium nitrate, glycine, and phenylalanine) were added to a mountain grassland soil at a range of concentrations. Soils were incubated at 21% and 1% of O2 content and at 10 oC and 20oC. Oxygen availability was a main factor controlling the reaction rates and temperature sensitivity of CO2 and N2O production. The temperature sensitivity of CO2 production was higher under aerobic versus oxygen-limited conditions, and the opposite dependency was observed for the N2O production. Substrate availability was a second factor affecting the temperature sensitivity of the processes leading to the production of these gases. The temperature response was reduced under substrate limitation. Apparent activation energy for aerobic CO2 production was similar (Ea ~ 30 kJ mol-1) for tested substrates, while Ea for anaerobic N2O production increased in the order phenylalanine < glycine < glucose + NO3- having values 45, 75, and 106 kJ mol-1, respectively. Commonly, the temperature sensitivity of N2O production (2 < Q10 <4.5) was much higher than that for CO2 (Q10 ≤ 1.5). Key words Temperature sensitivity, Michaelis-Menten kinetics, CO2 emission, nitrous oxide emission, glycine, oxygen availability
    Full-text · Article · Jul 2014 · Biology and Fertility of Soils
Show more