Sources of anterior gastric vagal efferent discharge in rats: an electrophysiological study

Brain Research Institute, University of California, Los Angeles, Los Ángeles, California, United States
Journal of the Autonomic Nervous System 02/1992; 37(1):29-37. DOI: 10.1016/0165-1838(92)90142-4
Source: PubMed


The source of vagal efferent discharge (VED) in the anterior branch of the gastric vagus was investigated in urethane-chloralose anesthetized rats using successive and selective vagal cuts. After cutting the right cervical vagus, the basal VEDs were increased in 15 out of 21 cases by 4-53% (median 18%). After both cervical vagi were cut, VEDs were reduced by 10-95% (median 90%) in 14 of 17 experiments and a subcervical basal VED was observed in all rats. Additional cut of the distal end of the anterior gastric branch did not induce a consistent effect. A small segment of subdiaphragmatic anterior gastric vagus (4-5 mm) was further isolated by a fourth cut at the proximal end of the anterior gastric vagus; abolition of the subcervical VED occurred in only 4 of 14 successful cuts whereas in the other 10 experiments, the VED was reduced by 38-94% (median 87%). Histological examination revealed the presence of neurons in a paraganglion lying within the isolated nerve segment. These findings indicate that the stomach not only receives VED descending directly from medullary vagal motor neurons (about 90%), but also (approximately 10%) from neural elements located between subcervical to upper abdomen levels (the 'subcervical VED') and/or between the bifurcation of the accessory celiac branch to the gastro-esophageal junction (the 'residual VED'). In rats there is little crossed gastric vagal innervation, in agreement with anatomical observations, although there is a robust inhibitory influence from contralateral vagal afferents on medullary vagal motor neurons.

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    • "On the other hand, the gastric afferents are much more complex. Although sensory fibers from the stomach run through the greater splanchnic nerves and enter the spinal cord around T 7 T 9 , the vagi nerves also carry sensory fibers both in rats and humans (Wei et al., 1992). The pathophysiology of AD is complex. "
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    ABSTRACT: The central nervous system action of bombesin to influence basal gastric vagal efferent discharge (GVED) was investigated in urethane-anesthetized rats. Bombesin (62, 620, and 6200 pmol) injected intracisternally (IC) decreased GVED to 78 +/- 10%, 50 +/- 4%, and 43 +/- 3% of preinjection levels, respectively. Bombesin (620 pmol) injected IV also reduced GVED to 36 +/- 6%. Pretreatment with bombesin monoclonal antibody 2A11 completely prevented the decrease in GVED induced by bombesin (620 pmol) given IV but not IC. These data indicate that both IC and IV injections of bombesin decrease basal GVED, and that the inhibitory effect of IC injection represents a central nervous system-mediated action.
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