Effects of inoculation with Phanerochaete chrysosporium on remediation of pentachlorophenol-contaminated soil waste by composting

PROCESS BIOCHEMISTRY (Impact Factor: 2.52). 06/2011; 46(6):1285-1291. DOI: 10.1016/j.procbio.2011.02.018


The effect of inoculation with Phanerochaete chrysosporium (P. chrysosporium) during different fermentation phases on remediation of pentachlorophenol (PCP)-contaminated soil waste (PSW) was investigated over 60 days. This was accomplished by evaluating physico-chemical and biochemical properties of composts, as well as bacterial community composition using denaturing gradient gel electrophoresis (DGGE). Results showed that the inoculations could significantly enhance composting efficiency and PCP removal. The best degree of maturity and highest PCP removal occurred in Run C (inoculation during the second fermentation phase) were compared with Runs A (control treatment) and B (inoculation during the first fermentation phase). A positive effect on production of manganese peroxidase (MnP) and lignin peroxidase (LiP) was found in inoculated runs, especially in Run C, while the production of laccase (Lac) was limited by P. chrysosporium inoculants. As a result of DGGE analysis, the compost bacterial community composition was altered by different inoculations, as indicated by the differences between the final composts. This study highlights the different effects of the inoculations on remediation performance of PSW. The inoculation during the second fermentation phase is more effective than that during the first fermentation phase

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Available from: Lin Tang, Apr 02, 2014
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    • "Some microorganisms characteristics such as: development under extreme conditions (Kumar et al., 2011), fast growth, low cultivation cost and capacity of transforming a wide variety of non-natural chemicals are very important to their use in bioremediation (Simões and Tauk-Tornisielo, 2005; Yu et al., 2011). Dash et al. (2013) reported that marine microorganisms may show these characteristics, producing unique enzymes in different environmental conditions. "
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    ABSTRACT: In this study, the marine-derived fungi Aspergillus sydowii DL6A, Apergillus versicolor DL5A, Cladosporium oxysporum DL5G, Fusarium proliferatum DL11A and Trichoderma harzianum CBMAI 1677 isolated from marine ascidian Didemnun ligulum were evaluated according to their growth in the presence of pentachlorophenol (PCP). The colonies were assessed in 10, 20, 30, 40 and 50 mg L−1 of PCP in a solid culture medium (3% malt). The fungus T. harzianum CBMAI 1677 showed the best growth at 50 mg L−1, which suggests its potential for biodegradation, therefore, this strain was selected for quantitative experiments in 3% malt liquid medium (initial concentration of 20 mg L−1 of PCP) using a validated method. After 7 d of incubation, PCP was not detected and an increasing concentration of pentachloroanisole (PCA) and 2,3,4,6-tetrachloroanisole (2,3,4,6-TeCA) was observed. In a second step, T. harzianum CBMAI 1677 was employed in the biodegradation of PCA and 2,3,4,6-TeCA in a liquid medium. It was observed that both PCA and 2,3,4,6-TeCA were also biodegraded. T. harzianum CBMAI 1677 is a potential strain for bioremediation studies since it was able of biodegrade not only PCP at 20 mg L−1, but also its main metabolites PCA and 2,3,4,6-TeCA.
    Full-text · Article · Apr 2015 · Biocatalysis and Agricultural Biotechnology
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    • "dation of PCP in soil , water and some bioreactors by P . chrysosporium and the application of inoculation with P . chrysosporium to treat hazardous wastes has showed to be effective in PCP degradation and remediation of PSW at laboratory - scales but less information is available about the effect of inoculation , due to lack of further research ( Yu et al . , 2011 ) . There is not much information published on the influence of pH on laccase production , but most reports indicated that initial pH levels has to be set between pH 4 . 5 and pH 6 . 0 prior to inoculation , the levels were not controlled during most cultivation ( Thurston , 1994 ; Gochev and Krastanov , 2007 ) . The optimal temperature"
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    ABSTRACT: Screening was done for the isolation of effective lignin degraders from the forest soil samples, by providing lignin as a carbon source through the enrichment method, which leads to the isolation of 8 effective fungal isolates among 14 isolates. Submerged fermentation was done for the production of ligninolytic enzymes with the effective microorganisms by providing Guiaicol as a carbon source. The assay of laccase, lignin peroxidise activity and specific activity was done after the incubation intervals of 2, 4, 6, 7, 8, 10 and 12 days at 27±2°C under shake culture condition. Partially purified protein content was estimated by using Lowry's method. Pleurotus sp. and Phanerochaetae chrysosporium are more effective at the 2nd and 7th days of incubation for the production of laccase and lignin peroxidases among the effective isolates.
    Full-text · Article · Jun 2014
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    • "The culture was then filtrated and washed thrice with 200 mL of 0.12 M HaH 2 PO 4 –Na 2 HPO 4 buffer. After moisture measurement, those prepared mycelia were used as inoculants (Yu et al. 2011). "
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    ABSTRACT: This research was conducted to distinguish between the separate effects of the Phanerochaete chrysosporium inoculation and sample property heterogeneity induced by different inoculation regimes on the indigenous bacterial communities during agricultural waste composting. P. chrysosporium was inoculated during different phases. The bacterial community abundance and structure were determined by quantitative PCR and denaturing gradient gel electrophoresis analysis, respectively. Results indicated a significant stimulatory effect of P. chrysosporium inoculation on the bacterial community abundance. The bacterial community abundance significantly coincided with pile temperature, ammonium, and nitrate (P < 0.006). Variance partition analysis showed that the P. chrysosporium inoculation directly explained 20.5 % (P = 0.048) of the variation in the bacterial communities, whereas the sample property changes induced by different inoculation regimes indirectly explained up to 35.1 % (P = 0.002). The bacterial community structure was significantly related to pile temperature, water-soluble carbon (WSC), and C/N ratio when P. chrysosporium were inoculated. The C/N ratio solely explained 7.9 % (P = 0.03) of the variation in community structure, whereas pile temperature and WSC explained 7.7 % (P = 0.026) and 7.5 % (P = 0.034) of the variation, respectively. P. chrysosporium inoculation affected the indigenous bacterial communities most probably indirectly through increasing pile temperature, enhancing the substrate utilizability, and changing other physico-chemical factors.
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