Evaluation of Antihyperglycemic Activity of Citrus limetta Fruit Peel in Streptozotocin-Induced Diabetic Rats

Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal 700032, India.
ISRN Endocrinology 07/2011; 2011. DOI: 10.5402/2011/869273
Source: PubMed


The present paper aims to evaluate antihyperglycemic activity of methanol extract of Citrus limetta fruit peel (MECL) in
streptozotocin-induced (STZ; 65mg/kg b.w.) diabetic rats. Three days after STZ induction, diabetic rats received MECL orally
at 200 and 400 mg kg−1 body weight daily for 15 days. Glibenclamide (0.5mg kg−1 p. o.) was used as reference drug. Blood glucose
levels were measured on 0th, 4th, 8th, and 15th days of study. Serum biochemical parameters namely, SGOT, SGPT and ALP
were estimated. The TBARS and GSH levels of pancreas, kidney, and liver were determined. MECL significantly (P < 0.001) and
dose dependently normalized blood glucose levels and serumbiochemical parameters, decreased lipid peroxidation, and recovered
GSH as compared to those of STZ control. The present paper infers that in STZ-induced diabetic Wistar rats, C. limetta fruit peel
demonstrated a potential antihyperglycemic effect which may be attributed to its antioxidant property.

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Available from: Sriparna Kundusen, Feb 12, 2014
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    • "A 2009 study examining extracts from a Korean Citrus fruit called Dangyuja (Citrus grandis) found that it holds great potential for controlling blood glucose levels in diabetic patients [8] . Citrus limetta fruit peel demonstrated a potential anti-hyperglycemic effect in streptozotocin induced diabetic rats [9] . "
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    ABSTRACT: Objective: To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr.fruit in α-amylase inhibitory activity (in vitro) and hypoglycemic activity in normal and glucose induced hyperglycemic rats (in vivo). Methods: Fruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents, α-amylase inhibitory activity, and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats. Results: Presence of saponin, steroid and terpenoid were identified in the extract. The results showed that fruit extract had moderate α-amylase inhibitory activity [IC50 value=(3.638±0.190) mg/mL] as compared to acarbose. Moreover at 500 mg/kg and 1 000 mg/kg doses fruit extract significantly (P<0.05 and P<0.01 respectively) reduced fasting blood glucose level in normal rats as compared to glibenclamide (5 mg/kg). In oral glucose tolerance test, 500 mg/kg dose significantly reduced blood glucose level (P<0.05) at 2 h but 1 000 mg/kg dose significantly reduced blood glucose level at 2 h and 3 h (P<0.05 and P<0.01 respectively) whereas glibenclamide (5 mg/kg) significantly reduced glucose level at every hour after administration. Overall time effect is also considered extremely significant with F value=23.83 and P value=0.0001 in oral glucose tolerance test. Conclusion: These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.
    Full-text · Article · Jun 2014 · Asian Pacific Journal of Tropical Biomedicine
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    Full-text · Article · Jan 2013
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    ABSTRACT: The polar constituents of peels from Citrus limetta variety Risso (Rutaceae) were investigated by a combination of two complementary chromatographic techniques consisting of preparative high-speed countercurrent chromatography (HSCCC), and off-line LC–ESI–MS/MS analysis to design a two-dimensional metabolite profile. Countercurrent chromatography (CCC) using solely immiscible solvent systems allowed the fractionation of principal components and an enrichment of minor concentrated metabolites from a crude polar solvent partition of C. limetta peels for subsequent structural identification by LC–ESI–MS/MS analysis. The combination of two very different chromatographic techniques resulted in lower detection limits for electrospray mass-spectrometry and revealed eighty-five compounds, including three abscisic acid derivatives, five limonoid glycosides, twenty-six dihydro-cinnamic and cinnamic acid glycosides, eleven flavanone glycosides, seven flavone glycosides, seventeen flavonol glycosides, including limocitrol and limocitrin derivatives. As a chemocharacteristic for C. limetta metabolites, many of the detected structures were linked to single and multiple 3-hydroxy-3-methyl-glutaryl (HMG) substitutions. C. limetta peels are a by-product of juice production, and not only the antioxidant fractions but also some of the fortified compounds could be used for food and pharmaceutical purposes.
    No preview · Article · Sep 2014 · Food Chemistry