Maintaining organoleptic quality of minimally processed cabbage using 6-benzylaminopurine.
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Questions & Answers about this publication
- How to find out Drug Mode of Action?
I am interested in knowing the mode of action of a drug named 'Benyzladenine'. It's a plant cytokinin and helps in treatment of diseases caused by exon skipping/defective splicing of pre-mRNA for example, Familial dysautonomia. This plant cytokinin somehow helps in exon inclusion (mutated exon, which in other case will be skipped and lead to the disease onset) but the actual mechanism of how it works is unknown.
I was wondering if only the experimental approaches are a solution to this problem or by using computational methods I can predict this drug's mode of action? A web-link to a patent talking about this topic is given.
6-Benzylaminopurine, benzyl adenine or BAP is a first-generation synthetic cytokinin that elicits plant growth and development responses, setting blossoms and stimulating fruit richness by stimulating cell division. It is an inhibitor of respiratory kinase in plants, and increases post-harvest life of green vegetables. Influence of cytokinin as 6-benzylaminopurine (BAP) in combination with other methods on postharvest green color retention on broccoli heads and asparagus spears, showed positive results for quality retention. [1, 2].
Till now the mode of action of this chemical is not well understood. For example the study by Shroder et al. entitled " Correlative polar auxin transport to explain the thinning mode of action of benzyladenine on apple" did not reveal a clear explanation for a possible mode of action of BA. similarly the study by Yuan et al. did not demonstrate a clear mode of action. The conclusion from this study was carbohydrate supply and source-sink relationships.
Since the active site of BA is not known yet, it will be difficult (may be impossible) to predict its mode of action by computational methods.
In order to predict a mode of action, you need to have QSAR, SAR, Inhibitors, amino acid sequencing and other related data.
Therefore, more experiments should be done before any computational predictions.
1. Siddiqui, M. W.; Bhattacharjya, A.; Chakraborty, I. and Dhua, R. S. 2011. 6-benzylaminopurine improves shelf life, organoleptic quality, and health-promoting compounds of fresh-cut broccoli florets. Journal of Scientific and Industrial Research, 70 (6): 461- 465.http://nopr.niscair.res.in/bitstream/123456789/11927/1/JSIR%2070%286%29%20461-465.pdf
2. Siddiqui, M.W.; Bhattacharjya, A. and Chakraborty, I. 2011. Maintaining organoleptic quality of minimally processed cabbage using 6-benzylaminopurine. International Symposium on System Intensification towards Food and Security, Feb. 24- 27, 2011, Kalyani, Nadia, West Bengal, India, p. 405.https://www.researchgate.net/publication/215441021_Maintaining_organoleptic_quality_of_minimally_processed_cabbage_using_6-benzylaminopurine
Hoping this will be helpful,