Determination of phenol, m-, o- and p-cresol, p-aminophenol and p-nitrophenol in urine by high-performance liquid chromatography

Laboratorio Biomed, (Concesio) BS, Italy.
Journal of Chromatography A (Impact Factor: 4.17). 01/1991; 535(1-2):311-6. DOI: 10.1016/S0021-9673(01)88957-3
Source: PubMed


A method for the biological monitoring of human exposure to aromatic hydrocarbons, nitrocompounds, amines and phenols has been developed. Phenol, cresols, p-aminophenol, p-nitrophenol and their glucorono- or sulpho-conjugates, were quantified by HPLC; 4-chlorphenol was added as internal standard. After enzymatic hydrolysis, the free compounds were extracted with an organic solvent and analyzed by an isocratic HPLC Perkin Elmer system at ambient temperature and at a flow-rate of 1 ml/min. The column was a reversed-phase Pecosphere 3 x 3 C18 Perkin Elmer; the mobile phase was a 30:70:0.1 (v/v/v) methanol-water-orthophosphoric acid mixture and the chromatogram was monitored at 215 nm. Identification was based on retention time and quantification was performed by automatic peak height determination, corrected for the internal standard. The recovery was ca. 95% for phenol and cresols; 90% for p-nitrophenol; 85% for p-aminophenol; the coefficients of variance were less than 6% within analysis (n = 20) and less than 10% between analysis (n = 20). The detection limits, at a signal/noise ratio of 2, were 0.5 mg/l for phenol and cresols and 1 mg/l for p-aminophenol and p-nitrophenol.

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    • "It is focused a significant attention for the development of simple, reliable, and ultrasensitive in various detection methodology based on undoped nanomaterials. Generally, toxic 4-NPh determination is performed using conventional chromatographic techniques, such as gas-chromatography (GC) [47] [48], high-performance liquid chromatography (HPLC) [49] [50], liquid chromatography (LC) connected with mass-spectroscopy. Universal screening method for the determination of US Environmental Protection Agency, phenols at nanolevel in water samples by on-line solid-phase extraction–highperformance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry within a single-run [51] and capillary-electrophoresis [52]. "
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    ABSTRACT: We have prepared low-dimensional silver oxide nanoparticles (NPs) by a sono-chemical methodusing reducing agents in alkaline medium. The resulting NPs were characterized by UV/vis and FT-IRspectroscopy, X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS), X-ray energydispersive spectrometry (XEDS), and field-emission scanning electron microscopy (FESEM). They weredeposited on a flat-polycrystalline gold electrode (AuE, surface area, 0.0216 cm2) to give a sensor witha fast response toward 4-nitrophenol (4-NPh) in liquid phase. The sensor also displays good sensitiv-ity and long-term stability, and enhanced electrochemical performances. The calibration plot is linear(r2= 0.9873) over the large concentration range (LDR, 1.0 �M to 0.5 mM). The sensitivity and detectionlimit is calculated to ∼4.740 �A cm−2mM−1and ∼0.19 �M (signal-to-noise ratio, at a SNR of 3), respec-tively. We also discuss possible future prospective uses of this metal oxide nanomaterials in terms ofchemical sensing.
    Full-text · Article · Dec 2013 · Electrochimica Acta
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    • "There is focused a significant attention for the development of simple, reliable, and ultra-sensitive in various detection methodology based on codoped nanomaterials. Generally, the detection of toxic 4-nitrophenol is consummated using chromatographic techniques, such as gas-chromatography [27,28], high-performance liquid chromatography [29,30], liquid chromatography connected with mass-spectroscopy [31], and capillary-electrophoresis [32]. Electrochemical technique, which can offer fast, reliable, and direct real-time monitoring is one of the most utilized methods in the determination of nitro-phenolic stuffs. "
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    ABSTRACT: Background Semiconductor doped nanostructure materials have attained considerable attention owing to their electronic, opto-electronic, para-magnetic, photo-catalysis, electro-chemical, mechanical behaviors and their potential applications in different research areas. Doped nanomaterials might be a promising owing to their high-specific surface-area, low-resistances, high-catalytic activity, attractive electro-chemical and optical properties. Nanomaterials are also scientifically significant transition metal-doped nanostructure materials owing to their extraordinary mechanical, optical, electrical, electronic, thermal, and magnetic characteristics. Recently, it has gained significant interest in manganese oxide doped-semiconductor materials in order to develop their physico-chemical behaviors and extend their efficient applications. It has not only investigated the basic of magnetism, but also has huge potential in scientific features such as magnetic materials, bio- & chemi-sensors, photo-catalysts, and absorbent nanomaterials. Results The chemical sensor also displays the higher-sensitivity, reproducibility, long-term stability, and enhanced electrochemical responses. The calibration plot is linear (r2 = 0.977) over the 0.1 nM to 50.0 μM 4-nitrophenol concentration ranges. The sensitivity and detection limit is ~4.6667 μA cm-2 μM-1 and ~0.83 ± 0.2 nM (at a Signal-to-Noise-Ratio, SNR of 3) respectively. To best of our knowledge, this is the first report for detection of 4-nitrophenol chemical with doped Mn2O3-ZnO NPs using easy and reliable I-V technique in short response time. Conclusions As for the doped nanostructures, NPs are introduced a route to a new generation of toxic chemo-sensors, but a premeditate effort has to be applied for doped Mn2O3-ZnO NPs to be taken comprehensively for large-scale applications, and to achieve higher-potential density with accessible to individual chemo-sensors. In this report, it is also discussed the prospective utilization of Mn2O3-ZnO NPs on the basis of carcinogenic chemical sensing, which could also be applied for the detection of hazardous chemicals in ecological, environmental, and health care fields.
    Full-text · Article · Mar 2013 · Chemistry Central Journal

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