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Effect of exogenous hydrogen peroxide on human erythrocytes

Authors:
MGiuliana Tozzi Ciancarelli at Università degli Studi dell'Aquila
MGiuliana Tozzi Ciancarelli
Chiara Di Massimo at FH Campus Wien
Chiara Di Massimo
M C D'Orazio
M C D'Orazio
M C D'Orazio
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A Mascioli
A Mascioli
A Mascioli
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Abstract

Circulating erythrocytes are drastically susceptible to peroxidative reactions. To examine the extent of the damage induced by exogenous H2O2 we limited the catalase activity in order to study the extent of lysis, the lipid peroxidation and namely the behaviour of membrane micro-viscosity. Our data showed that the erythrocytes can efficiently scavenge exogenous H2O2 without significant damage of the cells and/or their membranes. These findings could confirm the important role of the erythrocytes as extracellular-antioxidant defense.
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Biblioteca richiedente: Facoltà di Medicina e Chirurgia- Università degli Studi dell'Aquila
Data richiesta: 03/07/2015 10:59:35
Biblioteca fornitrice: Biblioteca Interdipartimentale di Medicina - Biblioteca Biomedica
Data evasione: 07/07/2015 12:12:59
Titolo rivista/libro: Cellular and molecular biology
Titolo articolo/sezione: Effect of exogenous hydrogen peroxide on human erythrocytes
Autore/i: Tozzi-Ciancarelli MG, Di Massimo C, D'Orazio MC, Mascioli A, Di Giulio A, Tozzi E
ISSN: 0145-5680
DOI:
Anno: 1990
Volume: 36
Fascicolo: 1
Editore:
Pag. iniziale: 57
Pag. finale: 64
... Because blood H 2 O 2 is significantly elevated in sepsis, the bidirectionality of H 2 O 2 cell-membrane permeability implies a similar H 2 O 2 load within the intracellular compartment (van Asbeck et al. 1995). Due to its significant reductive capacity derived from reduced thiols in serum albumin and erythrocyte glutathione, whole blood normally functions as a physiological redox sink for H 2 O 2 diffusing from the intracellular compartment into the systemic circulation (Roche et al. 2008;Cha and Kim 1996;Tozzi-Ciancarelli et al. 1990). Consequently, systemically elevated H 2 O 2 indicates depletion of reductive capacity in whole blood and within the intracellular compartment (Lyons et al. 2001). ...
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