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Protective immunization against experimental Bacteroids (Porphyromonas) gingivalis infection

Department of Oral Biology, School of Dental Medicine, State University of New York, Buffalo 14214.
Infection and Immunity (Impact Factor: 3.73). 11/1990; 58(10):3394-400.
Source: PubMed

ABSTRACT

The effects of immunization in modulating the pathogenesis of Bacteroides (Porphyromonas) gingivalis infection in a murine model system were examined. BALB/c mice were immunized by intraperitoneal injection with B. gingivalis ATCC 53977 (one injection per week for 3 weeks), or with a lithium diiodosalicylate (LIS) extract (one injection per week for 3 weeks), or with lipopolysaccharide (LPS; one intravenous or intraperitoneal injection) from this same strain. Two weeks after the final immunization, the mice were challenged by subcutaneous injection of B. gingivalis ATCC 53977. Mice immunized with bacteria had no secondary lesions and no septicemia, whereas mice immunized with LIS extract had few secondary lesions and no septicemia. Mice immunized with LPS and nonimmunized mice demonstrated secondary abdominal lesions and septicemia after challenge. Bacterial cells and LIS extract, but not LPS, induced serum antibody and antigen reactive lymphocytes, as measured by enzyme-linked immunosorbent assay, immunoblot, Western immunoblot transfer, and in vitro lymphoproliferative responses. The present study suggests that immunization with a LIS extract or whole cells may induce a protective response against experimental B. gingivalis infection.

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    • "A number of studies have reported the predominance of serum IgG2 antibodies in periodontal disease subjects that lack strong complement fixation and opsonic properties so that the humoral response to P. gingivalis may be ineffective in clearing this organism (Lopatin et al., 1992; Whitney et al., 1992). LPS and other carbohydrate antigens tend to stimulate IgG2 rather than IgGI or IgG3, which are generally produced in response to protein antigens (Schenk and Michaelson, 1987; Ogawa et al., 1990). There have also been reports demonstrating the low avidity of anti-P. "
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    ABSTRACT: In recent years, the phenotypic characterization of T cell subsets has given way to a functional dichotomy based essentially on their cytokine profiles. In this context, the CD4+ helper T cell subset has been shown to consist of two types, termed Th1 and Th2. In general, Th1 cells produce interleukin (IL)-2 and interferon (IFN)-gamma, while Th2 cells characteristically produce IL-4, IL-5, and IL-6. The major function of the Th1 subset is to mediate delayed-type hypersensitivity reactions and their secondary function is suppression of B cell activity. In contrast, the major function of the Th2 subset is to provide B cell help, while their secondary function is cell-mediated immune suppression. A similar dichotomy has also been described for CD8+ T cells. The role that these functional T cell subsets and their cytokines play in terms of their protective and nonprotective outcomes in a variety of infectious and oral diseases is reviewed.
    Full-text · Article · Feb 1994 · Critical Reviews in Oral Biology & Medicine
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    • "A number of studies have reported the predominance of serum IgG2 antibodies in periodontal disease subjects that lack strong complement fixation and opsonic properties so that the humoral response to P. gingivalis may be ineffective in clearing this organism (Lopatin et al., 1992; Whitney et al., 1992). LPS and other carbohydrate antigens tend to stimulate IgG2 rather than IgGI or IgG3, which are generally produced in response to protein antigens (Schenk and Michaelson, 1987; Ogawa et al., 1990). There have also been reports demonstrating the low avidity of anti-P. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Inrecent years,thephenotypic characterization ofTcell subsets hasgiven way toa functional dichotomy basedessentially on their cytokine profiles. Inthis context, theCD4+helper Tcell subset hasbeen showntoconsist oftwotypes, termed ThIandTh2. Ingeneral, ThIcells produce interleukin (IL)-2 andinterferon (IFN)-gamma, while Th2 cells characteristicaly produce IL-4, IL-5, andIL-6. Themajor function oftheThisubset istomediate delayed-type hypersensitivity reactions andtheir secondary function issuppression ofBcell activity. Incontrast, themajor function oftheTh2subset istoprovide B cell help, while their secondary function iscell- mediated immunesuppression. A similar dichotomy hasalso beendescribed forCD8+Tcells. Therole that these functional Tcell subsets andtheir cytokines playintermsoftheir protective andnonprotective outcomes ina variety ofinfectious andoral diseases isreviewed.
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