The lysozyme enhancer: Cell-specific activation of the chicken lysozyme gene by a far upstream DNA element

The EMBO Journal (Impact Factor: 10.43). 05/1986; 5(4):719-24.
Source: PubMed


The chicken lysozyme gene is constitutively expressed in macrophages and controlled by steroid hormones in the oviduct. We have investigated the influence of the 5' noncoding region of this gene on its cell-specific transcriptional activation. In transient transfection experiments we have identified a far-upstream cell-specific enhancer element 6.1 kb 5' to the transcriptional start site of the lysozyme gene. Transcription from the lysozyme gene promoter is induced by this element in a position- and orientation-independent manner in lysozyme-producing myeloid cells (HBCI), but not in non-producing chicken embryo fibroblasts (CEF-38). The enhancer region correlates with a DNase-hypersensitive chromatin site which is only detectable in cells of tissues in which the lysozyme gene is transcribed. We suggest that this far-upstream element is involved in the tissue-specific control of lysozyme gene activity.

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Available from: Manfred Theisen
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    • "hages, neutrophils, and eosinophilic granular cells in skin epidermis and intestine (Fletcher & White, 1976;Sveinbjørnsson et al., 1996). In higher vertebrates, lysozyme c is present in polymorphonuclear and mononuclear phagocytes, and in Paneth cells, which are specialized intestinal epithelial cells (Keshav et al., 1991;Dohrman et al., 1994).Theisen et al. (1986) andShort et al. (1996)reported that the chicken genome, like the human, has a single lysozyme c gene, which is highly expressed in the oviduct and macrophages.Nile et al. (2004)found that three lysozyme genes were expressed in the chicken small intestine by villous enterocytes to fulfil complimentary roles in protecting the intestine, lysoz"
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    ABSTRACT: Necrotic enteritis is a worldwide poultry disease caused by the overgrowth of Clostridium perfringens in the small intestine. An experiment with a 2x2 factorial design (supplementation with or without 40 mg lysozyme/kg diet for chickens challenged with or without C. perfringens) was conducted to investigate the inhibitory efficacy of exogenous lysozyme against intestinal colonization by C. perfringens in chickens subject to oral inoculation of C. perfringens type A on days 17 to 20. The C. perfringens challenge resulted in significant increase of C. perfringens, Escherichia coli and Lactobacillus populations in the ileum, bacteria translocation to the spleen, the intestinal lesion scores , There was significantly lower intestinal lysozyme activity in the duodenum and jejunum and weight gain during days 14 to 28 of the experiment. The addition of exogenous lysozyme significantly reduced the concentration of C. perfringens in the ileum and the intestinal lesion scores, inhibited the overgrowth of E. coli and Lactobacillus in the ileum and intestinal bacteria translocation to the spleen, and improved intestinal lysozyme activity in the duodenum and the feed conversion ratio of chickens. These findings suggest that exogenous lysozyme could decrease C. perfringens colonization and improve intestinal barrier function and growth performance of chickens.
    Preview · Article · Feb 2010 · Avian Pathology
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    • "Lee) Tel.: +82-42-821-5779 Fax: +82-42-825-9754 E-mail: 2010년 10월 5일 투고 2010년 10월 29일 심사완료 2010년 12월 13일 게재확정 Theisen et al., 1986 "
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    ABSTRACT: Single nucleotide polymorphisms (SNPs) in chicken lysozyme (LYZ) gene were investigated in this study. The identification of SNPs in both exon and intron in LYZ gene has led to understanding of evolution for the domestic chicken populations. A total of 24 samples from two Korean native commercial chicken populations (CCPs) were used for the initial identification of SNPs by mixing three DNA samples for sequencing experiments. By comparing with red jungle fowl (RJF), two commercial chicken populations have 18 common polymorphisms. Between two commercial chicken populations, 15 polymorphisms were identified. Of the 33 polymorphisms identified, two indels (21 and 4 bp) were found. Whereas, only one polymorphism in exon 2 at the bp position 1426 was a non-synonymous substitution (p.Ala49Val), indicating the amino acid changes. The identified non-synonymous substitution (p.Ala49Val) is located close to the catalytic sites of the enzyme, which might affect its activity. In our investigation, the polymorphisms in LYZ gene can provide broad ideas for the variation of Korean native chicken populations from the ancestor of chicken breeds as well as the some biological functions of the LYZ gene.
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    • "Other HSP70/CAT plasmids contain the promoter region of the human HSP70 gene (Kaddurah-Daouk et al. 1987). pLYSCAT constructs contain the promoter region of the chicken lysozyme gene (Theisen et al. 1986). (534) A sequence to which v-myb protein binds in vitro (Biedenkapp et al. 1988). "
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    ABSTRACT: The retroviral oncogene v-myb encodes a nuclear, sequence-specific DNA-binding protein. To investigate the possibility that v-myb encodes a transcriptional regulator, we used a transient cotransfection assay to explore the potential of v-myb to influence the expression of other genes. We found that expression of a chicken lysozyme promoter/CAT gene construct was activated by v-myb in the presence of myb-specific binding sites. Action was not observed with a truncated v-myb protein lacking its DNA-binding domain. We also observed that expression of a hybrid human HSP70 promoter/CAT gene, lacking myb-specific binding sites, was activated by v-myb. However, in this case, the truncated v-myb protein, which lacked its DNA-binding domain, also activated HSP70/CAT expression, indicating that trans-activation of this gene construct was independent of the sequence-specific DNA-binding activity of the v-myb protein. These observations suggest that v-myb encodes a trans-activator and that activation of gene expression occurs by two different mechanisms, one of which involves specific binding of v-myb protein to the regulated gene.
    Preview · Article · Nov 1989 · Genes & Development
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