Evolution of the casein multigene family conserved sequences in the 5′ flanking and exon regions. Nucleic Acids Res. 14, 1883-1902

Baylor College of Medicine, Houston, Texas, United States
Nucleic Acids Research (Impact Factor: 9.11). 03/1986; 14(4):1883-902. DOI: 10.1093/nar/14.4.1883
Source: PubMed


The rat α- and bovine αs1-casein genes have been isolated and their 5′ sequences determined. The rat α-, β-, γ- and bovine αs1-casein genes contain similar 5′ exon arrangements in which the 5′ noncoding, signal peptide and casein klnase phosphorylatlon
sequences are each encoded by separate exons. These findings support the hypothesis that during evolution, the family of casein
genes arose by a process involving exon recruitment followed by intra-genic and intergenic duplication of a primordial gene.
Several highly conserved regions in the first 200 base pairs of the 5′ flanking DNA have been identified. Additional sequence
homology extending up to 550 base pairs upstream of the CAP site has been found between the rat α- and bovine αs1-casein sequences. Unexpectedly, the 5′ flanking promoter regions are conserved to a greater extent than both the entire mature
coding and intron regions of these genes. These conserved 5′ flanking sequences may contain potential cis regulatory elements
which are responsible for the coordinate expression of the functionally-related casein genes during mammary gland development.

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