Article

Targeting of a foreign protein to chloroplasts by fusion to the transit peptide of ribulose 1,5-bisphosphate carboxylase. Nature 313: 358-363

Nature (Impact Factor: 41.46). 01/1985; 313(6001):358-63. DOI: 10.1038/313358a0
Source: PubMed

ABSTRACT

Chimaeric genes can be constructed which fuse the transit peptide of a small subunit of the chloroplast-located ribulose 1,5-bisphosphate carboxylase with a bacterial protein. The fusion protein is translocated into chloroplasts and cleaved in a similar way to the small subunit polypeptide precursor.

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    • "Since the main target of bromoxynil lies in the thylakoïd membrane of the chloroplast, we qucstionncd whcther the localisation of nitrilase inside the cell had an influence on bromoxynil resistance. We used the transit peptide of both the maize and sunflowcr SSU to target the nitrilase inside the chloroplast stroma (Schreier et al., 1985 ;Van den Broeck et al., 1985). The construction was made in such a way that the native enzyme should be rcleased in the stroma aftcr protcolytic cleavage at the cysteinc-mcthionine jonction bctween the transit peptide and the nitrilasc. "
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