JOURNAL OF BACTERIOLOGY, Jan., 1967, p. 59-64
American Society for
Vol. 93, No.
Printed in U.S.A.
TADASHI ARAI, TATSUYA ITO, AND YASUMASA KOYAMA
Department of Antibiotics, Institute ofFood Microbiology, Chiba University, Narashino, Chiba, and
Pharmaceutical Faculty, Chiba University, Chiba, Japan
Received for publication 18 July 1966
Antimicrobial activity of a crude aflatoxin preparation and of aflatoxin B1
studied. They were found inactive against common gram-positive and gram-nega-
tive bacteria at a concentration of 100 jAg/rn1. Both samples of aflatoxin did, how-
ever, exhibit antimicrobial activity, though narrow and limited, against various
strains of Streptomyces and Nocardia. The antibiotic action of aflatoxin
confirmed by bioautogram after thin-layer chromatography. Among seven strains
ofnmicroorganisms, including aflatoxin-sensitive and -resistant strains, N. asteroides
IFM 8 was found to reduce aflatoxin B1, in addition
components in the crude preparation.
to other minor fluorescent
Although numerous data concerning biological
activity of aflatoxins (12, 13), the toxic secondary
metabolites of Aspergillus flavus, have been thus
into the growth
strate inhibited 12 species of the genus Bacillus,
one species of Clostridium, and one of Strepto-
myces among the 329 microorganisms tested at a
concentration of 30lAg/ml.The crude preparation
on an alumina column
TABLE 1. Antimicrobial activity ofcrude aflatoxin and aiflatoxin B1"
Minimal inhibitory concn (pug/mi)
Bacillus subtilis PCI 219...............75b
B. cereus ATCC1072.OO
Streptomyces vinaceus IFM 1017...........25
S. olivoreticuli IFM 1018...............10
S. lavendulae IFM 1025................25
S. roseochromogenes IFM10950
S. virginiae IFM 1028................25
S. halstedii IFM 1032................50
S. netropslis IFM 1035................25
S. aureofaciens IFM 1042...............100
S. antibioticus IFM10925
S. griseoluteus IFM 1055...............25
Nocardia leishmanii IFM 27.............100
N. asteroides IFM150
N. asteroides IFM225
N. asteroides IFM325
N. asteroides IFM8>100
N. coeliaca IFM 30.................25
N. rangoonensis IFM 23...............
N. brasiliensis lFm 65................50
Medium: glucose-nutrient agar for Bacillus and Sabouraud agar(1%0glucose) for Streptomyces and
Nocardia. Incubation: 27 C for 72 hr for Streptomyces, 37 C for 24 hr for Bacillus, and 37 C for 72 hr for
bGrowth was retarded down by concentrations as low as 50 JAg/mi.
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