Fluorescence immunoassay

Human Pathlogy (Impact Factor: 2.77). 03/1984; 15(2):112-6. DOI: 10.1016/S0046-8177(84)80049-0
Source: PubMed


Fluorescence immunoassay is a sensitive technique that can be used in the measurement of many compounds, including drugs, hormones, and proteins; in the identification of antibodies; and in the quantification of antigens such as viral particles and, potentially, bacteria. Homogeneous fluorescence immunoassay, fluorescent excitation transfer immunoassay, fluorescence polarization immunoassay, solid-phase "dipstick" immunoassay, solid-phase microbead fluorescence immunoassay, substrate-labeled fluorescence immunoassay, and fluorescence immunoassays using internal reflectance spectroscopy or phycobiliprotein conjugates are reviewed.

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    • "Specific DNA sequences have been detected by hybridization assays using different materials such as biotin-avidin, protein-enzyme, and fluorescent dyes. However, these methods are limited by low signal intensity, rapid photobleaching as well as biosafety problems [6,7]. Fluorescent semiconductor nanocrystals, quantum dots (QDs) possess unique properties and have significant advantages (such as tunable band gap and extraordinary photostability) over the classic organic dyes. "
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    ABSTRACT: In the present study we describe sandwich design hybridization probes consisting of magnetic particles (MP) and quantum dots (QD) with target DNA, and their application in the detection of avian influenza virus (H5N1) sequences. Hybridization of 25-, 40-, and 100-mer target DNA with both probes was analyzed and quantified by flow cytometry and fluorescence microscopy on the scale of single particles. The following steps were used in the assay: (i) target selection by MP probes and (ii) target detection by QD probes. Hybridization efficiency between MP conjugated probes and target DNA hybrids was controlled by a fluorescent dye specific for nucleic acids. Fluorescence was detected by flow cytometry to distinguish differences in oligo sequences as short as 25-mer capturing in target DNA and by gel-electrophoresis in the case of QD probes. This report shows that effective manipulation and control of micro- and nanoparticles in hybridization assays is possible.
    Full-text · Article · Jul 2009 · Sensors
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    ABSTRACT: A review is given on the novel non-radioactive digoxigenin:anti-digoxigenin (DIG) bioanalytical indicator system. After a general introduction on direct and indirect indicator systems based on previous non-radioactive indicator reactions as well as in vitro and in vivo amplification procedures the principle of the new digoxigenin:anti-digoxigenin technology is demonstrated. The novel system is based on the specific high-affinity interaction between the cardenolide digoxigenin from Digitalis plants and a digoxigenin-specific antibody coupled with a reporter group. A variety of methods for digoxigenin modification of nucleic acids, proteins and glycans are presented. In addition, various applications of the novel non-radioactive indicator system in a variety of direct or indirect detection approaches with either insoluble or soluble substrates are described. It is also shown that with these applications alternative reaction formats are used which are partly characterized by additional amplification steps.
    No preview · Article · Jul 1991 · Molecular and Cellular Probes
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