Article

A new colorimetric technique for the estimation of Vitamin C using Folin Phenol Reagent

Department of Biochemistry, Panjab University, Chandigarh-160 014, India
Analytical Biochemistry (Impact Factor: 2.22). 12/1982; 127(1):178-82. DOI: 10.1016/0003-2697(82)90162-2
Source: PubMed
ABSTRACT
A new colorimetric technique for the estimation of ascorbic acid by using Folin phenol reagent has been developed. The absorption maximum of the color developed by the interaction of ascorbic acid with Folin reagent is 760 nm. The technique obeys the Beer-Lambert law up to a concentration of 45 μg ascorbic acid as shown by the standard curve. The color developed has been found to be stable up to 18 h. Recovery experiments showed that the technique is almost 100% efficient. The development of the color is not obstructed by glucose, glutathione, bovine serum albumin, urea, cysteine, adenine, guanine, cytosine, uracil, sulfosalicylic acid, thymol, or oxyhemoglobin, which are compounds suspected of interfering in routine analysis. The technique is simple, quick, and efficient and can be employed for the estimation of ascorbic acid in a wide variety of biological materials.

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    • "No. A006-2, A005, Nanjing Jiancheng Bioengineering Institute, China). Moreover, the concentrations of vitamin A in brain homogenate samples were quantified according to the previously described methods [23][24][25]. Level of vitamin C and vitamin E in brain homogenate samples were measured using detection kits according to the manufacturers' introduction (Catal. No. A009, A008, Nanjing Jiangcheng Bioengineering Institute, Nanjing, China). "
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    • "Glutathione was determined according to Anderson (1985). Ascorbic acid was estimated according to colorimetric technique described by Jagota and Dani (1982), based on the interaction between ascorbic acid and Folin reagent. "
    Full-text · Dataset · May 2016
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    • "Glutathione was determined according to Anderson (1985). Ascorbic acid was estimated according to colorimetric technique described by Jagota and Dani (1982), based on the interaction between ascorbic acid and Folin reagent. "
    Full-text · Dataset · May 2016
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