A new colorimetric technique for the estimation of Vitamin C using Folin Phenol Reagent

Department of Biochemistry, Panjab University, Chandigarh-160 014, India
Analytical Biochemistry (Impact Factor: 2.22). 12/1982; 127(1):178-82. DOI: 10.1016/0003-2697(82)90162-2
Source: PubMed


A new colorimetric technique for the estimation of ascorbic acid by using Folin phenol reagent has been developed. The absorption maximum of the color developed by the interaction of ascorbic acid with Folin reagent is 760 nm. The technique obeys the Beer-Lambert law up to a concentration of 45 μg ascorbic acid as shown by the standard curve. The color developed has been found to be stable up to 18 h. Recovery experiments showed that the technique is almost 100% efficient. The development of the color is not obstructed by glucose, glutathione, bovine serum albumin, urea, cysteine, adenine, guanine, cytosine, uracil, sulfosalicylic acid, thymol, or oxyhemoglobin, which are compounds suspected of interfering in routine analysis. The technique is simple, quick, and efficient and can be employed for the estimation of ascorbic acid in a wide variety of biological materials.

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    • "The ascorbic acid was estimated by using Folin phenol reagent using different concentrations of standard ascorbic acid (Jagota and Dani, 1982). The amount of ascorbic acid was expressed as mg g -1 fw. "
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    ABSTRACT: Centella asiatica (L.) Urban is a perennial herb with high utility in traditional medicines and pharmaceutical industries. In India the wild populations C. asiatica are overexploited at an unrestrained rate to cope up with its high market demands. The aim of this study was to identify potential chemotype of C. asiatica from different geographical region in India and optimized in vitro micropropagation protocol of the elite ecotype was established for commercial purposes. Five C. asiatica accessions were collected from different geographic locations and their active biological components were analyzed. Among these accessions total phenolic and flavonoid content combined with ascorbic acid was found to be significantly higher in CA-4 compared to other ecotypes. The free radical scavenging activity as well as reducing power of the CA-4 ecotype also corroborate the higher phenolic content. Therefore the elite ecotype CA-4 was selected for in vitro conservation and micropropagation. Shoot-tips explants was inoculated on Murashige and Skoog basal medium with different concentrations and combinations of cytokinins and auxins. Optimum multiplication was achieved on medium containing 6-benzyl-aminopurine (2.0 mg L –1) and indole-3-acetic acid (0.2 mg L –1). In vitro rooting of multiplied individual shoots was achieved on half strength Murashige and Skoog medium supplemented with IBA (1.5 mg L –1), with a maximum of 16.4 ± 0.22 roots. These micropropagated plants were successfully established in soil after hardening with 94.55 % survival rate.
    Full-text · Article · Sep 2015 · Journal of Applied Pharmaceutical Science
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    • "(ii) pH: the pH of pickle solution and pickled roselle calyces were determined using digital pH meter. (iii) Anthocyanin content: the amount of anthocyanin in the fresh and pickled roselle calyces were determined at 520 nm using spectrophotometer based on delphinidin-3-glucoside. (iv) Ascorbic acid: the amount of ascorbic acid were determined according to Jagota and Dani (1982) "
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    Full-text · Article · Sep 2014
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    • "In our study, NO produced following focal ischemia/reperfusion injury was quantified by measuring nitrite which is been a stable end product of NO (Jagota and Dani, 1982). A rise in the nitrite level is being evident in the region of ischemic insult of brain after 10 min following cerebral ischemia and declined to basal levels after 60 min (Stirling et al., 2005). "
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    ABSTRACT: Aim Aquaporin-4(AQP4) expression in brain with relation to edema formation following focal cerebral ischemia was investigated. Studies have shown that brain edema is one of the significant factors in worsening stroke outcomes. While many mechanisms may aggravate brain injury, one such potential system may involve AQP4 up regulation in stroke patients that could result in increased edema formation. Post administration of melatonin following ischemic stroke reduces AQP4 mediated brain edema and confer neuroprotection. Materials and Methods An in-silico approach was undertaken to confirm effective melatonin-AQP4 binding. Rats were treated with 5mg/kg, i.p. melatonin OR placebo at 30 min prior, 60 min post and 120 min post 60 minutes of MCAO followed by 24 hour reperfusion. Rats were evaluated for battery of neurological and motor function tests just before sacrifice. Brains were harvested for infarct size estimation, water content measurement, biochemical analysis, apoptosis study and western blot experiments. Key findings Melatonin at sixty minutes post ischemia rendered neuroprotection as evident by reduction in cerebral infarct volume, improvement in motor and neurological deficit and reduction in brain edema. Furthermore, ischemia induced surge in levels of nitrite and MDA were also found to be significantly reduced in ischemic brain regions in treated animals. Melatonin potentiated intrinsic antioxidant status, inhibited acid mediated rise in intracellular calcium levels, decreased apoptotic cell death and also markedly inhibited protein kinase C influenced AQP4 expression in the cerebral cortex and dorsal striatum. Significance Melatonin confer neuroprotection by Protein Kinase C mediated AQP4 inhibition in ischemic stroke.
    Full-text · Article · Apr 2014 · Life sciences
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