Hepatoprotective activity of carrot (Daucus carota L.) against carbon tetrachloride intoxication in mouse liver. J Ethnopharmacol
Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India. Journal of Ethnopharmacology
(Impact Factor: 3).
08/1995; 47(2):69-74. DOI: 10.1016/0378-8741(95)01254-B
The effect of carrot extract on carbon tetrachloride (CCl4)-induced acute liver damage was evaluated. The increased serum enzyme levels (viz., glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, lactate dehydrogenase, alkaline phosphatase, sorbitol and glutamate dehydrogenase) by CCl4-induction were significantly lowered due to pretreatment with the extract. The extract also decreased the elevated serum bilirubin and urea content due to CCl4 administration. Increased activities of hepatic 5'-nucleotidase, acid phosphatase, acid ribonuclease and decreased levels of succinic dehydrogenase, glucose-6-phosphatase and cytochrome P-450 produced by CCl4 were reversed by the extract in a dose-responsive way. Results of this study revealed that carrot could afford a significant protective action in the alleviation of CCl4-induced hepatocellular injury.
Available from: Dharmendra Singh
- "Experimental animals exposed with CCl 4 , transaminases activity (AST, ALT), ALP, ACP, γ-GTP, LDH and SDH were increased remarkably in plasma by the release of these enzymes from hepatic paranchymal cells, which were indicating a considerable hepatocellular injury by oxidative stress (Bishayee et al., 1995; Achliya et al., 2004). Oral treatment with silymarin and E. littorale extract attenuated these increased enzyme activities produced by CCl 4 and a subsequent recovery towards normalization of these enzymes strongly suggests the possibility of silymarin and E. littorale extract being able to improve the condition of the hepatocytes so as to cause accelerated regeneration of paranchymal cells, thus protecting against membrane fragility decreasing the leakage of marker enzymes into the circulation stabilization of serum-total bilirubin, total protein and albumin levels through the administration of silymarin and the extract are further a clear indication of the improvement of the functional status of the hepatic cells (Bishayee et al., 1995; Achliya et al., 2004). Thus the results of the present investigation are clearly demonstrate that various biochemical changes, produced in the serum and liver of rats by CCl 4 treatment, were significantly restored by the oral administration of silymarin and by E. littorate extract dose dependently. "
Available from: Fatma El-Demerdash
- "Moreover, rosemary provide protection against hepatotoxins by enhancing the functioning of the hepatic antioxidant defense system (Ip and Ko, 1996), inhibiting biosynthesis of cytochrome p 450 (Rao and Misra, 1998) and preventing LPO (Malo et al., 2011). Additionally, rosemary can help in stabilizing hepatocellular membrane, enhancing protein biosynthesis (Lin et al., 1999), decreasing the leakage of marker enzymes into the circulation, interfering with the microsomal activation of PAHs and/or accelerating detoxification (Bishayee et al., 1995). In agreement with the present study, Guti errez et al. (2009) and Sotelo-Felix et al. (2002) suggested that R. officinalis therapy, acting as an antioxidant and/or a free radical scavenger, can preserve cellular integrity and counteract the severe damage induced by carbon tetrachloride (CCl 4 ). "
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ABSTRACT: Coal tar is a significant product generated from coal pyrolysis. Coal tar can be utilized as raw materials for various industries. It is also a type of raw material from which phenols, naphthalenes, and anthracene can be extracted. The present study was designed to investigate the possibility of coal tar creosote to induce oxidative stress and biochemical perturbations in rat liver and the role of rosemary (Rosmarinus officinalis) in ameliorating its toxic effects. Male Wister Albino rats were randomly divided into four groups of seven each, group I served as control; group II treated with rosemary (10 mL of water extract/kg BW for 21 days), group III received coal tar creosote (200 mg/4 mL olive oil/kg BW for 3 days), and group IV treated with both rosemary and coal tar creosote. The administration of coal tar creosote significantly caused elevation in lipid peroxidation (LPO) and reduction in the activities of glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST). A significant decrease in reduced glutathione (GSH) content was also observed. Liver aminotransferases aspartate transaminase (AST) and alanine transaminase (ALT)] and alkaline phosphatase (AlP) were significantly decreased while lactate dehydrogenase (LDH) was increased. Rosemary pretreatment to coal tar creosote-treated rats decreased LPO level and normalized GPx, GR, SOD, CAT, and GST activities, while GSH content was increased. Also, liver AST, ALT, AlP, and LDH were maintained near normal level due to rosemary treatment. In conclusion, rosemary has beneficial effects and could be able to antagonize coal tar creosote toxicity. © 2014 Wiley Periodicals, Inc. Environ Toxicol, 2014.
Available from: Muhammad Asif
- "It is widely accepted that, in hepatic parenchyma cells, cytochrome P450-dependant monooxygenases convert the accumulated CCl4 into CCl3 radicals. In addition to the alkylation of cellular proteins, CCl3 attacks the polyunsaturated fatty acids to produce lipid peroxides that are responsible for the hepatotoxicity and alteration of hepatic enzyme levels . The disturbance of hepatocytic transport function during hepatic injury causes an altered permeability of the membrane leading to the leakage of enzymes from the cells , thus resulting in the reduction of the ALT, AST, and ALP levels in the hepatic cells and elevation of their levels in the serum . "
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ABSTRACT: Oxidative stress, lipid peroxidation, and transaminase reactions are some of the mechanisms that can lead to liver dysfunction. A time-dependent study was designed to evaluate the ability of silymarin (SLN) and glycyrrhizin (GLN) in different dosage regimens to lessen oxidative stress in the rats with hepatic injury caused by the hepatotoxin carbon tetrachloride. Wistar male albino rats (n = 60) were randomly assigned to six groups. Group A served as a positive control while groups B, C, D, E, and F received a dose of CCl4 (50% solution of CCl4 in liquid paraffin, 2 mL/kg, intraperitoneally) twice a week to induce hepatic injury. Additionally, the animals received SLN and GLN in different doses for a period of six weeks. CCl4 was found to induce hepatic injury by significantly increasing serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and thiobarbituric acid reactive substances while decreasing total protein and the activities of reduced glutathione, superoxide dismutase, and catalase. Treatment with various doses of SLN and GLN significantly reduced ALT, AST, ALP, and TBARS levels and increased GSH, SOD, and CAT levels. Our findings indicated that SLN and GLN have hepatoprotective effects against oxidative stress of the liver.
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