Hepatoprotective activity of carrot (Daucus carota L.) against carbon tetrachloride intoxication in mouse liver

ArticleinJournal of Ethnopharmacology 47(2):69-74 · August 1995with115 Reads
DOI: 10.1016/0378-8741(95)01254-B · Source: PubMed
Abstract
The effect of carrot extract on carbon tetrachloride (CCl4)-induced acute liver damage was evaluated. The increased serum enzyme levels (viz., glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, lactate dehydrogenase, alkaline phosphatase, sorbitol and glutamate dehydrogenase) by CCl4-induction were significantly lowered due to pretreatment with the extract. The extract also decreased the elevated serum bilirubin and urea content due to CCl4 administration. Increased activities of hepatic 5'-nucleotidase, acid phosphatase, acid ribonuclease and decreased levels of succinic dehydrogenase, glucose-6-phosphatase and cytochrome P-450 produced by CCl4 were reversed by the extract in a dose-responsive way. Results of this study revealed that carrot could afford a significant protective action in the alleviation of CCl4-induced hepatocellular injury.
    • "This hepatotoxicity is due to free radical CCl 3 which is metabolite. It lessens alkalization of cellular proteins and other macromolecules with simultaneous attack on polyunsaturated fatty acids to produce lipid peroxide (Bishayee et al., 1995). "
    Full-text · Article · Aug 2016
    • "Daucus carota L. (Umbelliferae), commonly known as carrot, is an important plant cultivated worldwide for its nutritive roots. Carrot has been used in traditional medicine due to a wide range of reported pharmacological effects, including antibacte- rial [1], antifungal [2], antihelminthic, and hepatoprotective [3] effects. Carrot roots contain a wide variety of high-value compounds including phenolic compounds such as coumarins [4] and p-hydroxybenzoic acid [5], volatile terpenoids [6] and several isoprenoid compounds like carotenoids [7], phytosterols [8] chlorophylls [9] and tocopherols [10] . "
    [Show abstract] [Hide abstract] ABSTRACT: In this work, suspension-cultured cells of _Daucus carota_ were used to evaluate the effect of β-cyclodextrins on the production of isoprenoid and phenolic compounds. The results showed that the phytosterols and phenolic compounds were accumulated in the extracellular medium (15.100 μg L−1 and 477,46 μg L−1, respectively) in the presence of cyclodextrins. Unlike the phytosterol and phenolic compound content, β-carotene (1.138,03 μg L−1), lutein (25.949,54 μg L−1) and α-tocopherol (8.063,82 μg L−1) chlorophyll _a_ (1.625,13 μg L−1) and _b_ (9.958,33 μg L−1) were mainly accumulated inside the cells. Therefore, cyclodextrins were able to induce the cytosolic mevalonate pathway, increasing the biosynthesis of phytosterols and phenolic compounds, and accumulate them outside the cells. However, in the absence of these cyclic oligosaccharidic elicitors, carrot cells mainly accumulated carotenoids through the methylerythritol 4-phosphate pathway. Therefore, the use of cyclodextrins would allow the extracellular accumulation of both phytosterols and phenolic compounds by diverting the carbon flux towards the cytosolic mevalonate/phenylpropanoid pathway.
    Article · Jun 2016
    • "Reduction in total protein content can be deemed as a useful index of severity of hepatocellular damage[70]. In the present study, CCl 4 intoxication reduced the serum total protein level which is attributed to the initial damage produced and localized in the endoplasmic reticulum which results in the loss of cytochrome P-450 enzymes leading to its functional failure with a decrease in protein synthesis and accumulation of triglycerides leading to fatty liver[71].Previous studies have showed that administration of CCl 4 to a diversity of animal types results in a rapid reduction in protein synthesis in the liver[66,72,73]. The pretreatment of MECC+CCL 4 restored the total protein level that suggests the stabilization of endoplasmic reticulum leading to protein synthesis[59]. "
    [Show abstract] [Hide abstract] ABSTRACT: Liver disease has become one of the serious health problems as it is exposed to many kinds of xenobiotics and therapeutic agents. Certain medicinal plants had been used to cure some liver diseases. The present study aimed to evaluate the hepatoprotective effect of Methanolic Extract of Caralluma cicatricose (MECC) in rabbits with acute liver injury induced by carbon tetrachloride at a single dose of 1.25 ml/kg b.w. as a mixture with olive oil. MECC was administered in doses of (0.5, 1 and 1.5 g/kg b.w.) via oral gavage by intragastric tube for 8 days. The SGOT, SGPT, ALP, total protein, creatinine, blood urea nitrogen and blood glucose level were measured and histopathology of liver was performed. The results of the rabbits treated with MECC were compared with that of Liv-52. C. cicatricosa showed a significant dose-dependent reduction (P< 0.05) in the hepatic enzymes levels, blood urea nitrogen, blood glucose level and improvement of serum protein. The histopathological studies in the liver of rabbits also supported that C. cicatricosa extract markedly reduced the toxicity of CCl 4 and preserved the histoarchitecture of the liver tissue to near-normal structure. These results suggested that C. cicatricose may be acting as a natural hepatoprotective agent that prevents hepatic injury induced by CC1 4 and this may be due to its active phytoconstituents such as flavonoids and glycosides which present in the plant.
    Full-text · Article · Dec 2015
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