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The influence of anticoagulant, storage temperature and time on equine plasma lactate concentration

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... 15 In the present study, the increase in lactate might have been a result of production of lactate by the erythrocytes and leucocytes in the blood sample by way of anaerobic glycolysis. 16 The activities of AST in serum of the Brazilian donkey breed reference ranges in the present study were similar to activities stated for Indian, 5 Italian, 5 and Catalonian 7 donkeys. Serum AST activities were lower than those determined for the United States 3 and Poitou 5 donkeys, but they were extremely higher than those determined for Albino 4 and United Kingdom 6 donkeys. ...
Article
SummarySeventeen serum biochemical variables were determined in 40 donkeys of the Brazilian breed (34 females and 6 males) aged from 3 to 19 years. Mean ± standard deviation (SD) (minimum–maximum) values, obtained by automated analysis, were as follows: glucose, 58.35 ± 10.40 (44.00–90.00) mg/dL; cholesterol, 88.41 ± 9.86 (73.58–124.26) mg/dL; serum protein, 6.82 ± 0.40 (6.00–7.52) g/dL; albumin, 3.13 ± 0.21 (2.65–3.69) g/dL; creatinine, 1.80 ± 0.14 (1.51–2.19) mg/dL; urea, 24.25 ± 5.37 (14.12–34.39) mg/dL; lactate, 20.10 ± 4.58 (12.99–33.47) mg/dL; aspartate aminotransferase (AST), 295.81 ± 62.79 (173.71–466.07) IU/L; creatine kinase (CK), 158.00 ± 76.94 (51.69–440.33) IU/L; γ-glutamil-transferase (GGT), 45.82 ± 13.34 (26.17–86.38) IU/L; lactate dehydrogenase (LDH), 576.02 ± 156.32 (213.53–1162.81) IU/L; alkaline phosphatase (AP), 345.36 ± 65.90 (227.25–490.16) IU/L; calcium (Ca), 8.54 ± 0.18 (8.19–8.90) mg/dL; phosphorus (P), 2.76 ± 0.38 (1.99–3.97) mg/dL; chloride (Cl), 106.05 ± 3.20 (99.00–112.00) mEq/L; sodium (Na), 121.50 ± 4.14 (116.00–132.00) mEq/L; and potassium (K), 3.70 ± 0.42 (2.80–4.40) mEq/L. Comparisons of biochemical ranges obtained for the Brazilian donkey breed with reference ranges for other donkey breeds suggested that most values were similar. Biochemical values determined in the present study serve as reference ranges for donkey populations and can be used for health control and diagnosis of diseases.
... Similarly, blood lactate concentration did not appear to change significantly in previous studies on human, 17 equine, 18 and feline 19 blood samples stored in NaF/Ox. The stability of plasma lactate concentration in blood samples preserved in NaF/Ox allowed comparison of direct lactate measurements obtained on the portable system (Lactate Scout) with those obtained within 1 hour after sampling on an automated analyzer (Konelab). ...
Article
Measurement of blood lactate concentration has become a common practice in canine medicine. However, the accuracy of portable lactate monitors has not been reported in dogs. The aim of this study was to evaluate the accuracy and precision of a portable analyzer (Lactate-Scout) in measuring canine blood lactate concentration. A preliminary study was performed to assess the effects of sample storage time and temperature on plasma lactate concentration. Blood samples obtained from 6 canine patients at our hospital were divided into 8 aliquots and stored at 4 degrees C and 20 degrees C; plasma lactate was measured in duplicate with a spectrophotometric system (Konelab) at 0, 30, 60, 120, and 240 minutes after the blood collection. Values were compared with those obtained immediately after blood collection. Lactate values obtained by the portable method also were compared with those obtained by the reference spectrophotometric analyzer on blood samples collected from 48 additional canine patients. There was no significant effect of storage time (P = .89) or temperature (P = .51) on plasma lactate levels. The correlation between lactate values measured with the Lactate-Scout and the Konelab method was r = .98 (slope = .81, 95% confidence interval = .73-.87; intercept = .20, 95% confidence interval = .13-.31). The level of agreement between the 2 methods was generally good for mean lactate concentrations <5 mmol/L. However, at higher lactate concentrations (5 of 48 samples), the values recorded by the Lactate-Scout analyzer were lower than those measured by the Konelab method. The Lactate-Scout analyzer is reliably comparable to a reference method for measuring whole blood lactate concentration in dogs; however, caution should be used when interpreting lactate values of 5 mmol/L and higher.
Thesis
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This study was carried out in two farms in Ismailia governorate. A total number of 221 buffalo calves; 65 from the first farm classified as control, acute bronchitis and chronic bronchitis; and 156 from the second farm classified as control, acute bronchitis, chronic bronchitis and pneumonic cases. Calves were of both sex aged between one to 90 days. The clinical respiratory score (CRS) was recorded for each individual animal. Animal having six or more of CRS were considered sick. Arterial blood samples were collected for measurement of blood gas analysis. Also venous blood samples were collected from diseased animals for measurement of serum lactate, proteinogram, protein electrophoresis and serum electrolytes. The study revealed that newborn buffalo calves suffered from bronchitis and pneumonia showed signs of polypnia, mucopurulent nasal and occular discharge, coughing, rattling sound, crackles and wheezes. The study revealed also that newborn calves showed a significant hypoxemia, hyperlactatemia, hyponatremia and hyperkalemia.
Article
The aim of this study was to investigate correlations between lactate concentrations in equine whole blood and plasma measured with Accusport1 and Yellow Springs Instruments (YSI2) (2300) methods. The effect of packed cell volume (PCV) on the accuracy of Accusport was also investigated. Blood samples were collected from Thoroughbred horses at 5-10 min intervals after a treadmill exercise test. Blood was added to NaEDTA (for PCV measurement) and to 2 tubes containing lithium heparin anticoagulant (for lactate assays). At concentrations greater than 10 mmol/l, Accusport1 greatly underestimated the blood lactate concentration. Accusport1 also underestimated blood lactate concentration when PCV were greater than 53%. Accusport1 accurately measured plasma lactate concentration in the range 0.8-20 mmol/l.
Article
The purpose of this study was to evaluate the accuracy of a portable blood lactate analyser for bovine blood and to study the relevance of plasma lactate concentration in the prognosis of bovine pulmonary disorders. Measurements with the portable analyser were highly correlated (R = 0.94, P<0.0001) with the measurements of the reference method but significantly different (P<0.0001). The portable apparatus slightly overestimated plasma lactate concentration compared to the reference method (bias = + 0.412). Plasma lactate measurements on 109 calves suffering from acute bronchopneumonia showed increasing lactate concentrations with severity of the disease. A plasma lactate concentration higher than 3.6 mmol/L or 4 mmol/L, measured with the reference method and the portable analyser respectively, appeared to be a reliable prognostic indicator for mortality within 24 h. Consequently, this measurement could be very helpful to decrease economic losses in cases of bovine respiratory disease, by avoiding unnecessary treatment costs on cattle with poor prognosis.
Article
Thesis (doctoral)--Universiteit te Utrecht, 1990.
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