Article

Fluorescence Spectroscopy: A Diagnostic Tool for Cervical Intraepithelial Neoplasia (CIN)

Biomedical Engineering Program, University of Texas, Austin 78712.
Gynecologic Oncology (Impact Factor: 3.77). 02/1994; 52(1):31-8. DOI: 10.1006/gyno.1994.1007
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ABSTRACT

A spectroscopic system incorporating a pulsed nitrogen laser, an optical fiber probe, and an optical multichannel analyzer was utilized to record fluorescence spectra of the intact cervix at colposcopy. Spectra were obtained from 66 colposcopically normal areas and 49 histologically abnormal areas (5 pathologic inflammation, 21 HPV infection, 9 CIN I, 10 CIN II, and 4 CIN III) in 28 patients. The resulting spectra could be used to differentiate histologically abnormal tissues from colposcopically normal tissues with a sensitivity, specificity, and positive predictive value of 92, 90, and 88%. Furthermore, CIN could be differentiated from nonneoplastic abnormal tissues with a sensitivity, specificity, and positive predictive value of 87, 73, and 74%. These results suggest that laser-induced fluorescence can be used in the recognition and differential diagnosis of CIN at colposcopy.

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    • "Based on the report of Alfano and his group on the detection of dental carries and subsequently cancer using native fluorescence, the field of optical biopsy has been given importance in diagnostic oncology [4] [5]. In this context, many reported on the characterisation and discrimination of various neoplastic conditions of cells and tissues from their normal counterpart using steady state native fluorescence spectroscopy [6]. However, only limited studies are available on the characterization of biofluids using native fluorescence spectroscopy in diagnostic oncology. "

    Full-text · Dataset · Feb 2014
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    • "Based on the report of Alfano and his group on the detection of dental carries and subsequently cancer using native fluorescence, the field of optical biopsy has been given importance in diagnostic oncology [4] [5]. In this context, many reported on the characterisation and discrimination of various neoplastic conditions of cells and tissues from their normal counterpart using steady state native fluorescence spectroscopy [6]. However, only limited studies are available on the characterization of biofluids using native fluorescence spectroscopy in diagnostic oncology. "
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    ABSTRACT: Urine is one of the diagnostically important bio fluids, as it has many metabolites and some of them are native fluorophores. There may be a variation in the distribution and the physiochemical properties of the fluorophores during any metabolic change and pathologic conditions. Native fluorescence spectroscopy has been considered as a promising tool to characterize the fluorophores present in the urine. In this study, we aimed at characterizing the urine of both normal and patients with confirmed cancer using steady state and time-resolved fluorescence spectroscopy at 280 nm and 350 nm excitation. It is observed that the metabolites indoxyl sulphate and neopterin and its derivatives are responsible for altered spectral signatures at 280 nm, and 350 nm excitation. The overall spectral data were subjected to Principal Component Analysis and the resultant components were used as input in the linear discriminant analysis. As a total, 84% and 81.8% of samples were correctly classified at 280 nm and 350 nm respectively.
    Full-text · Article · Mar 2013 · Proceedings of SPIE - The International Society for Optical Engineering
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    • "Fluorescence spectroscopy is a type of electromagnetic spectroscopy which analyzes fluorescence from a sample. The sample is excited by using a beam of light which results in emission of light of a lower energy resulting in an emission spectrum which is used to interpret results [5]. Fluorescence correlation spectroscopy (FCS), a technique basically used for spatial and temporal analysis of molecular interactions of extremely low concentrated biomolecules in solution. "
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    ABSTRACT: There are many diagnostic techniques and methods available for diagnosis of medically important microorganisms like bacteria, viruses, fungi and parasites. But, almost all these techniques and methods have some limitations or inconvenience. Most of these techniques are laborious, time consuming and with chances of false positive or false negative results. It warrants the need of a diagnostic technique which can overcome these limitations and problems. At present, there is emerging trend to use Fluorescence spectroscopy as a diagnostic as well as research tool in many fields of medical sciences. Here, we will critically discuss research studies which propose that Fluorescence spectroscopy may be an excellent diagnostic as well as excellent research tool in medical microbiology field with high sensitivity and specificity.
    Full-text · Article · Nov 2009 · Journal of Translational Medicine
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