Enterocyte transplantation using cell-polymer devices to create intestinal epithelial-lined tubes

Cornell University, Итак, New York, United States
Transplantation Proceedings (Impact Factor: 0.98). 03/1993; 25(1 Pt 2):998-1001.
Source: PubMed
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  • No preview · Article · Jul 1993 · Transplantation Reviews
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    ABSTRACT: Polyglycolic acid (PGA) fibre meshes are attractive candidates to transplant cells, but they are incapable of resisting significant compressional forces. To stabilize PGA meshes, atomized solutions of poly(l-lactic acid) (PLLA) and a 50/50 copolymer of poly(d,l-lactic-co-glycolic acid) (PLGA) dissolved in chloroform were sprayed over meshes formed into hollow tubes. The PLLA and PLGA coated the PGA fibres and physically bonded adjacent fibres. The pattern and extent of bonding was controlled by the concentration of polymer in the atomized solution and the total mass of polymer sprayed on the device. The compression resistance of devices increased with the extent of bonding, and PLLA bonded tubes resisted larger compressive forces than PLGA bonded tubes. Tubes bonded with PLLA degraded more slowly than devices bonded with PLGA. Implantation of PLLA bonded tubes into rats revealed that the devices maintained their structure during fibrovascular tissue ingrowth, resulting in the formation of a tubular structure with a central lumen. The potential of these devices to engineer specific tissues was exhibited by the finding that smooth muscle cells and endothelial cells seeded onto devices in vitro formed a tubular tissue with appropriate cell distribution.
    No preview · Article · Jan 1996 · Biomaterials
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    ABSTRACT: In the not too distant past, organ transplantation was considered a futuristic concept. However, things considered miraculous in one era may be merely remarkable in another (Langer and Vacanti, 1995). Over the last half of this century, development of techniques for tissue and organ transplantation have been revolutionary. Along with the tremendous advances in the field of transplantation, new problems have emerged needing solutions. It is in this context that the field of tissue engineering has emerged over the last decade (Vacanti and Vacanti, 1996).
    No preview · Chapter · Jan 1997
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