Regional Chromosomal Assignments for Four Members of the MADS Domain Transcription Enhancer Factor 2 (MEF2) Gene Family to Human Chromosomes 15q26, 19p12, 5q14, and 1q12–q23

Lawrence Livermore National Laboratory, Livermore, California, United States
Genomics (Impact Factor: 2.28). 11/1995; 29(3):704-11. DOI: 10.1006/geno.1995.9007
Source: PubMed


The MEF2 genes belong to the MADS box family of transcription factors and encode proteins that bind as homo- and heterodimers to a consensus CTA(T/A)4TAG/A sequence, which is present in the regulatory regions of numerous muscle-specific and growth-inducible genes. Sequence analysis of human MEF2 cDNA clones suggests that they arose from alternatively spliced transcripts of four different genes, termed MEF2A-D. We have mapped the MEF2 genes to human chromosomal regions by identifying unique sequences in the MEF2 cDNA clones and using these sequences as PCR primers on the DNA of human-rodent hybrid clone panels that are informative for different regions of the human genome. PCR primers were also used to identify individual YAC clones for two of the genes, MEF2A and MEF2C, and a PCR product was used to identify cosmid clones for MEF2B. Genetic and physical mapping information available from genome databases on markers contained within YAC and cosmid clones provided independent assignments for those genes. Inter-Alu PCR painting probes of YAC clones were used as probes for high-resolution chromosomal regional assignment by fluorescence in situ hybridization. The localization of MEF2A to chromosome 15q26, MEF2B to 19p12, MEF2C to 5q14, and MEF2D to 1q12-q23 verifies the existence of at least four distinct loci for members of this gene family.

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Available from: Ralf Krahe
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    • "In vertebrates, there are four mef2 genes, referred to as mef2a, -b, -c, and -d, that are located on different chromosomes (Pollock & Treisman 1991, Yu et al 1992, Breitbart et al 1993, Leifer et al 1993, Martin et al 1993, 1994, McDermott et al 1993, Hidaka et al 1995, Hobson et al 1995, Ticho et al 1996, Molkentin et al 1996b, Suzuki et al 1996, Morisaki et al 1997). There is a single mef2 gene in each of the genomes of Drosophila (Lilly et al 1994, Nguyen et al 1994), Caenorhabditis elegans (M Krause, personal communication), and sea urchins (J Venuti, personal communication). "
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