The prognosis of oral mucosal squamous cell carcinomas: A comparison of clinical and histopathological grading and of laminin and type IV collagen staining
Oral Medicine and Surgery Research Group, School of Dental Science, University of Melbourne.Australian Dental Journal (Impact Factor: 1.1). 05/1996; 41(2):83-6. DOI: 10.1111/j.1834-7819.1996.tb05918.x
Changes in the distribution of basement membrane components have been described in dysplastic lesions and in oral mucosal squamous cell carcinomas (OMSCC). The purpose of this study was to determine if these changes were related to pathological grade and if so, whether this had prognostic implications. Fifty formalin-fixed, paraffin-embedded specimens of OMSCC, with five or more years clinical follow-up, were studied using an immunoperoxidase technique for the detection of the basement membrane components, laminin and type IV collagen. The immunoreactivity of each component was evaluated and semiquantitatively scored as minimal, moderate or extensive and the results compared with the tumour size, node involvement and metastasis (TNM) clinical staging system and histopathological features. OMSCC were characterized by minimal or moderate staining with small islands of neoplastic cells frequently lacking staining for laminin and type IV collagen. Deposition of these components decreased with increased histopathological grade and absence of staining was more commonly associated with a poor prognosis. In particular the pattern of type IV collagen staining frequently differed from laminin staining. Neither of these parameters offered an advantage over TNM clinical staging with regard to prognosis. It was concluded that variations in laminin and type IV collagen immunoreactivity occurred in OMSCC and that high histopathological grade tumours with considerably diminished staining with anti-laminin and anti-type IV collagen carried a poor prognosis.
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- "Similar studies have been carried out previously. Firth and Reade showed that laminin distribution was continuous in epithelial hyperplasia while dysplastic lesions showed small focal breaks whose number increased in severe dysplasia. Kannan et al., reported a gradual increase in the frequency of laminin discontinuity from normal epithelium to hyperplastic, dysplastic and SCCs, with significant differences among groups. "
ABSTRACT: To determine the immunohistochemical (IHC) localization of basement membrane component laminin in histological grades of oral squamous cell carcinoma (OSCC). The purpose of this study was to demonstrate the loss of continuity of the basement membrane in OSCC using an antibody directed against laminin using advanced polymer staining system. A total of 30 cases of OSCC: 10 cases of well differentiated squamous cell carcinom (WDSCC), 10 cases of moderately differentiated squamous cell carcinoma (MDSCC), and 10 cases of poorly differentiated squamous cell carcinoma (PDSCC) were subjected to heat-induced antigen retrieval method using ethylene-di-amine-tetraacetic acid buffer in a microwave oven. Then the sections were stained with anti-laminin polyclonal antibody and visualized using super sensitive polymer horseradish peroxidase detection system. In each case, the integrity of the basement membrane laminin was assessed by using statistical analysis. Statistical analysis showed a decreased distribution of laminin from WDSCC to MDSCC to PDSCC (P value 0.0573). The intracytoplasmic staining of laminin gradually increased from WDSCC to MDSCC to PDSCC (P value 0.0198). WDSCC cases showed more laminin expression in basement membrane around the tumor islands and less loss of continuity compared to MDSCC and PDSCC cases suggesting a greater enzymatic degradation of basement membrane components in MDSCC and PDSCC than WDSCC. The loss of structural basement membrane laminin and the presence of laminin in the tumor cells of PDSCC cases suggest that laminin helps in tumor invasion. The expression of laminin in the basement membrane may be a useful parameter to evaluate tumor histologic differentiation and aggressiveness.
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- "The images of glomerular regions of kidneys were obtained by a camera microscope and were saved as a file. The intensity of collagen IV reaction was graded by two separate individual according to firth method (Firth et al., 1996). "
ABSTRACT: Basement membrane of glomerular mesangium (BMG) is a thin membrane which helps to support the capillary loops in a renal glomerulus and type IV collagen is require for complete BM formation during glomerulogenesis. In this investigation specific antibody of type IV collagen has been used in light microscopy to study development of BMG of the embryonic and postnatal mouse glomerular mesangium. In this study, 20 female Balb/C mice were selected randomly and finding vaginal plug was assumed as day zero of pregnancy. 12 pregnant mice were sacrified by cervical dislocation in one of gestational days 13-18, their fetuses were fixed and serially sectioned. Immunohistochemical Study for tracing of collagen type IV in BMG was carried out. The same processes were carried out for kidneys preparation of pups on 5, 10, 15 and 20 days after birth (2 mothers for each day). The result of the present study revealed that collagen IV reaction was weak on day 15 of gestation. The amount of collagen increased continuously until next days of fetal life and primary of 10 days postnatal in BMG. After this period, collagen IV showed no significant change in newborns. These data indicate that collagen IV appears just during the glomerular vasculogenesis and because of continuity and glomerular endothelial cell differentiation, type IV collagen, is the major structural protein in BMG, have been implicated in these processes.
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ABSTRACT: Objectives: Study the loss or reduction of the cellular adhesion mediated for E-cadherin in oral leukoplakias, oral squamous cell carcinomas and metastatic nodules. Study the loss of continuity of the laminin and collagen IV expression in the epithelial basal membrane from the biological development of the oral leukoplakias and oral carcinomas. Material and method: we have studied 124 samples of patient payees leukoplakias and oral carcinomas with diverse diagnosis that embrace from normal epithelium (13 samples), mild dysplasias (2), moderate dysplasias (12), �in situ� carcinomas (13), microinvasive carcinomas (11) oral squamous cell carcinomas (64 samples) and metastatic nodules (9). 7 blocks of tissue microarrays were built with needle of 2mm and was carried out a study by means of immunohistochemical technique for E-cadherin (clone 36, Biogenex), Laminin (078P, Biogenex) and Collagen IV (PHM12, Biogenex). Results: In Mild and Moderate Dysplasias the results present loss of E-cadherin, Laminin, and Collagen IV (20%) expression. �in situ� and microinvasive carcinomas, the results presented loss of E-cadherin expression (73%), and loss in Laminin and Collagen IV expression (57%). In the squamous cell carcinomas , we find E-cadherin underexpression (90%) and discontinuity in the Basal Membrane. (70%). All the metastatic nodules presented loss of E-cadherin expression and discontinuity in Laminin and Collagen IV expression. Conclusions: The loss of E-cadherin expression is increased when increasing the dysplasia grade of lesions. The loss of continuity in the laminin and Collagen IV expression follow a parallel evolution from dysplasias to metastatic nodules. The underexpression of the three markers has been significant in the evolution of the oral lesions. Objetivos: Estudiar la pérdida o reducción de la adhesión celular mediada por E-cadherina en leucoplasias, carcinomas epidermoides y metástasis ganglionares. Estudiar la pérdida de continuidad de la expresión de laminina y colágeno IV en la membrana basal epitelial en el desarrollo biológico de las leucoplasias y carcinomas orales. Material y metodo: Hemos estudiado 124 muestras de pacientes portadores de leucoplasias y carcinomas orales con diversos diagnósticos que abarcan desde epitelio normal (13 muestras), displasias leves (2), displasias moderadas (12), carcinomas in situ (13) carcinomas microinvasores (11) Carcinoma epidermoide oral (64 muestras) y metástasis ganglionar (9). Se construyeron 7 bloques de tissue microarrays con aguja de 2mm y se realizó un estudio mediante técnica inmunohistoquímica para E-cadherina (clona 36, T.D. ABD Company), Laminina (078P, Biogenex) y Colágeno IV (PHM12, Biogenex). Resultados: En Displasias Leves y Moderadas presentan pérdida de expresión de E-cadherina, Laminina, y Colágeno IV (20%). En Carcinomas in situ y Microinvasores, presentaron pérdida de expresión de E-cadherina (73%), y en Laminina y Colágeno IV (57%). En los carcinomas epidermoides, encontramos pérdida de expresión de E-cadherina (90%) y discontinuidad en la M. basal (70%). Todas las metástasis ganglionares presentaron pérdida de E-cadherina y discontinuidad en Laminina y Colágeno IV. Conclusiones: La pérdida de expresión de E-cadherina se incrementa al aumentar el grado de displasia de las lesiones. La perdida de continuidad en la expresión de laminina y Colágeno IV sigue una evolución paralela desde displasias a metástasis ganglionares. La disminución en la expresión de los tres marcadores ha sido significativa en la evolución de las lesiones orales.
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