A Neuropharmacological Analysis of PTZ-Induced Kindling in Mice

Depto. de Farmacologia, Universidade Federal do Rio Grande do Sul, RS, Brazil.
General Pharmacology 08/1998; 31(1):47-50. DOI: 10.1016/S0306-3623(97)00423-0
Source: PubMed


1. Glutamate seems to play a central role in epilepsy, and kindling is considered the most useful experimental model in revealing plastic changes associated with epileptic features. 2. The aim of this study was to optimize pentylenetetrazol (PTZ)-kindling conditions in mice and analyze glutamatergic changes associated with this phenomena. 3. A significant increase (85.7%) in seizuring animals was observed after four PTZ administrations, with all subjects presenting full seizures after five administrations. 4. PTZ kindling, but not acute seizure, significantly increased (169.8%) the specific binding of [3H]glutamate in the cerebral cortex. 5. The development of PTZ-induced kindling in mice was prevented by the coadministration of phenobarbital or diazepam. 6. This study indicates that mice can be used in a reliable model of PTZ-induced kindling and that, as in rats, the kindling increases the specific [3H]glutamate binding in the cerebral cortex, therefore allowing for screening new drugs that can interfere in the plastic changes believed to underlie epileptic phenomena.

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Available from: Elaine Elisabetsky, Jan 05, 2016
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    • "Electrical kindling usually stimulates a specific brain region, such as amygdala, hippocampus or other brain regions, via chronically implanted depth electrodes (32, 142, 143). Chemical kindling, such as pentylenetetrazoleis ultilized in some studies, but this method has been much less utilized than electrical kindling (144, 145). Pentylenetetrazole kindling involves repeated injection of pentylenetetrazole to cause gradual seizure development as a result of which a significant neuronal loss in hippocampus CA1 and CA3 structures have been observed (144). "
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    • "The development of chemical kindling induces permanent changes in mouse brain (Ekonomou et al., 2001; da Silva et al., 1998; De Luca et al., 2005). The amino acid level modifications in brain during seizure itself could result from both the kindling process and the physiological shock induced by the seizure. "
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