Article

Colonization of Vibrio pelagius and Aeromonas caviae in early developing turbot (Scophtalmus maximus L.) larvae

Foundation of Applied Research, University of Tromsø, Norway.
Journal of Applied Microbiology (Impact Factor: 2.48). 03/1998; 84(2):227-33. DOI: 10.1046/j.1365-2672.1998.00333.x
Source: PubMed

ABSTRACT

Polyclonal antisera made in rabbits against whole washed cells of Vibrio pelagius and Aeromonas caviae were used for detection of these bacterial species in the rearing water and gastrointestinal tract of healthy turbot (Scophthalmus maximus) larvae exposed to V. pelagius and/or Aer. caviae. The results demonstrated that this method is suitable for detection of V. pelagius and Aer. caviae in water samples and larvae at population levels higher than 10(3) ml-1 and 10(3) larva-1. Populations of aerobic heterotrophic bacteria present in the gastrointestinal tract of turbot larvae, estimated using the dilution plate technique, increased from approximately 4 x 10(2) bacteria larva-1 on day 3 post-hatching to approximately 10(5) bacteria fish-1 16 days post-hatching. Sixteen days after hatching, Vibrio spp. accounted for approximately 3 x 10(4) cfu larva-1 exposed to V. pelagius on days 2, 5 and 8 post-hatching. However, only 10(3) of the Vibrio spp. belonged to V. pelagius. When larvae were exposed to Aer. caviae on day 2 post-hatching, the gut microbiota of 5-day old larvae was mainly colonized by Aeromonas spp. (10(4) larva-1), of which 9 x 10(3) belonged to Aer. caviae. Later in the experiment, at the time when high mortality occurred, 9 x 10(5) Aer. caviae were detected. Introduction of V. pelagius to the rearing water seemed to improve larval survival compared with fish exposed to Aer. caviae and with the control group. It was therefore concluded that it is beneficial with regard to larval survival to introduce bacteria (V. pelagius) to the rearing water.

Download full-text

Full-text

Available from: Olav Vadstein, Oct 26, 2014
  • Source
    • "Oral administration provided advantages for prawns regardless of prawn size (Itami et al. 1998; Sakai 1999), such prawns can be treated at any stage of the culture period. Commonly, probiotics can be added directly into culture water (Gibson et al. 1998; Queiroz and Boyd 1998; Ringø and Vadstein 1998; Gram et al. 1999; Hai et al. 2009a) as water additives (Zhou et al. 2009; Cha et al. 2013), bathed in bacterial suspension (Hansen and Olafsen 1989; Smith and Davey 1993; Gram et al. 1999). The immersion method is also useful (Sung et al. 1994; Itami et al. 1998). "

    Full-text · Dataset · Jan 2016
  • Source
    • "There is no mention of Lactobacillus being even isolated from gut, gills or from the external surface of another widely consumed fish-grey mullets (Mugil cephalus) in the study of Nagvenkar et al. (2006) even though it does mention the absence of Azotobacter from the gut of M. cephalus. Ringo and Vadstein (1998) had reported that, LAB even though are present in the gut of human (as Bifidobacterium) and swine and birds (as Lactobacillus) and as Enterococcus in carnivores -are generally subdominant in the gut of the fishes and if at all, are mostly represented by the genus Carnobacterium (Gildberg and Mikkelsen, 1998). Isolate 246 showed good antimicrobial activity against the fish pathogens, while isolates 229, 232 and 234 displayed commendable activity against the shrimp pathogens (Table 2). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Emergence of antibiotic resistance among aquaculture pathogens has made it necessary to look into environment friendly, effective and sustainable methods such as probiotic and immunostimulants among others.. In the present study, LAB were isolated from the gut of fish species namely Rastrelliger kanagurta and analyzed for their antibacterial activity against various fish, shrimp and human pathogens. Different LAB species such as Lactobacillus plantarum, L. bulgaricus, L. brevis and L. viridiscens were encountered in the gut of R. kanagurta. Several strains showed good activity against fish, shrimp and human pathogens. LAB from the gut of such marine species may be developed as possible probiont for environment friendly health management of fresh water, estuarine and marine species currently exploited in aquaculture.
    Full-text · Article · Jan 2014
  • Source
    • "Kozasa (1986) reported that the spores of Bacillus toyoi increased the growth rate of yellowtail and reduced the mortality of Japanese eel that were infected by Edwardsiella sp. The Gramnegative Vibrio pelagius decreased the mortality of the turbot larvae Scophthalmus maximus challenged with Aeromonas caviae (Ringo and Vadstein, 1998). Gatesoupe (1991b) showed that Bacillus toyoi and Bacillus sp spores increased the growth rate of larval turbot introduced via the rotifer Brachionus plicatilis. "

    Full-text · Dataset · Jan 2013
Show more