The effects of antioxidant supplementation during Percoll preparation on human sperm DNA integrity [erratum appears in Hum Reprod 1998 Nov;13(11):3284]

University of Ulster, Aontroim, Northern Ireland, United Kingdom
Human Reproduction (Impact Factor: 4.57). 06/1998; 13(5):1240-7. DOI: 10.1093/humrep/13.5.1240
Source: PubMed


The integrity of sperm DNA is crucial for the maintenance of genetic health. A major source of damage is reactive oxygen species (ROS) generation; therefore, antioxidants may afford protection to sperm DNA. The objectives of the study were, first, to measure the effects of antioxidant supplementation in vitro on endogenous DNA damage in spermatozoa using the single cell gel electrophoresis (comet) assay and, second, to assess the effect of antioxidant supplementation given prior to X-ray irradiation on induced DNA damage. Spermatozoa from 150 patients were prepared by Percoll centrifugation in the presence of ascorbic acid (300, 600 microM), alpha tocopherol (30, 60 microM), urate (200, 400 microM), or acetyl cysteine (5, 10 microM). DNA damage was induced by 30 Gy X-irradiation. DNA strand breakage was measured using the comet assay. Sperm DNA was protected from DNA damage by ascorbic acid (600 microM), alpha tocopherol (30 and 60 microM) and urate (400 microM). These antioxidants provided protection from subsequent DNA damage by X-ray irradiation. In contrast, acetyl cysteine or ascorbate and alpha tocopherol together induced further DNA damage. Supplementation in vitro with the antioxidants ascorbate, urate and alpha tocopherol separately has beneficial effects for sperm DNA integrity.

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    • "In the same way, Long and Kramer (2003) demonstrated that the increase of lipid peroxidation during storage was unaffected by the addition of Vitamin E. However, other reports suggested a positive response of -tocopherol on equine semen during storage for 48 h at 5 • C (Ball and Vo, 2002) and in poultry (Donoghue and Donoghue, 1997). Hughes et al. (1998) found that in vitro treatment of sperm with 3 and 60 M -tocopherol reduced the magnitude of DNA damage. Jeong et al. (2009) suggested that tocopherol supplementation at 200 g/ml may protect sperm against excessive ROS generation by reducing lipid peroxidation and lowering the expression of apoptosis genes by reducing DNA fragmentation. "
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