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Prognosis for Dogs with Stage I or II Splenic Hemangiosarcoma Treated by Splenectomy Alone: 32 Cases (1991-1993)

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A retrospective analysis was performed on the case records of 32 dogs with Stage I or II splenic hemangiosarcoma that were treated by splenectomy alone and that survived the seven-day postoperative period. Median survival time for these 32 cases was 86 days (mean, 116 days; range, 14 to 470 days), and the one-year survival rate was estimated to be 6.25%. Survival was not influenced by signalment, presenting signs, stage of disease, or clinicopathological findings. The data provides a basis from which to evaluate adjuvant chemotherapy for splenic hemangiosarcoma that is confined to the spleen macroscopically.
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JOURNAL of the American Animal Hospital Association 417
Prognosis for Dogs with Stage I or II
Splenic Hemangiosarcoma Treated by
Splenectomy Alone: 32 Cases (1991–1993)
Carrie A. Wood, DVM
Antony S. Moore, MVSc
John M. Gliatto, VMD
Lee A. Ablin, DVM
Robert J. Berg, DVM
William M. Rand, PhD
From the Departments of Medicine
(Wood, Moore, Berg) and
Pathology (Gliatto),
Tufts University School of
Veterinary Medicine,
200 Westboro Road,
North Grafton, Massachusetts 01536; the
Angell Memorial Animal Hospital (Ablin),
350 South Huntington Avenue,
Boston, Massachusetts 02130; and
Tufts University Community Health
35 Kneeland Street,
Boston, Massachusetts 02111.
Doctor Wood’s current address is the
Department of Small Animal
Clinical Sciences,
College of Veterinary Medicine,
University of Minnesota,
C352 Veterinary Hospitals,
1365 Gortner Avenue,
St. Paul, Minnesota 55108.
Presented in part at the
14th Annual Conference,
Veterinary Cancer Society,
October, 1994.
A retrospective analysis was performed on the case records of 32 dogs with Stage I
or II splenic hemangiosarcoma that were treated by splenectomy alone and that
survived the seven-day postoperative period. Median survival time for these 32
cases was 86 days (mean, 116 days; range, 14 to 470 days), and the one-year
survival rate was estimated to be 6.25%. Survival was not influenced by signalment,
presenting signs, stage of disease, or clinicopathological findings. The data provides
a basis from which to evaluate adjuvant chemotherapy for splenic hemangiosarcoma
that is confined to the spleen macroscopically.
J Am Anim Hosp Assoc 1998;34:417–21.
Hemangiosarcoma is a malignant neoplasm originating from the vas-
cular endothelium that is characterized by widespread metastases and
poor survival rates. Hemangiosarcoma may arise from any site in the
body, but the spleen is affected most commonly in dogs.
primary sites include the right atrium and auricular appendage, liver,
lungs, kidney, skin, and the subcutis.
Splenic hemangiosarcoma affects older dogs, and the German shep-
herd dog may be at greater risk than other breeds for developing the
Clinical signs vary; acute collapse may follow rupture of
the highly vascular primary tumor, while other cases may show non-
specific signs of weakness, pallor, or anorexia.
Causes of death in
dogs with primary splenic hemangiosarcoma include exsanguination
when the primary tumor ruptures, metastatic disease, secondary dis-
seminated intravascular coagulation (DIC), and cardiac arrythmias.
Clinical staging has been a variable indicator of prognosis.
Stage I splenic hemangiosarcomas are confined to the spleen with no
evidence of metastases, while Stage II tumors may have ruptured and
may or may not have regional lymph-node involvement [see Appen-
Splenic hemangiosarcomas have been treated traditionally through
splenectomy, with poor survival times reported.
little data is available characterizing the prognosis and clinical pro-
gression of those cases receiving surgical excision of the primary
tumors which have no evidence of gross metastatic disease.
Small sample numbers, inclusion of animals that died in the immedi-
ate postoperative period, and inclusion of animals that received adju-
vant chemotherapy or immunotherapy make conclusions unreliable.
The reported survival times vary from 19 to 143 days. Comparison
among studies results in uncertain conclusions, as many reports do
not distinguish between stage of disease, tumor location, or treatment
In a large study of 104 cases of hemangiosarcoma, eight cases were
reported as Stage I disease (median survival time, 151 days), and 15
cases were reported as Stage II disease (median survival time, 143
days). However, three of the Stage I disease cases and seven of the
Stage II disease cases received either a mixed bacterial vaccine alone
418 JOURNAL of the American Animal Hospital Association September/October 1998, Vol. 34
or a vaccine in combination with various chemothera-
pies. Survival data was not presented for Stage I or II
disease cases treated with surgery alone.
Another report reviewed 92 cases of splenic hem-
angiosarcoma for which tissue samples were submit-
ted to a pathology laboratory service. Survival data
(median survival time, 19 days) was available for 59
cases. Data regarding clinical staging was not pre-
sented, and survival calculations included those ani-
mals that died in the immediate postoperative period.
A survey of 100 cases of splenectomies for various
indications reported survival data in four cases of
Stage I disease (median survival time, 91 days) and
one case of Stage II disease (survival time, 168 days).
The relatively small number of cases available makes
conclusions regarding overall survival times unreli-
able. Clinical staging was reported to be of no prog-
nostic significance; however, most cases that were
used for comparison received adjuvant chemo-
A more recent retrospective analysis of 1,480 cases
of canine splenic disease included data from 22 cases
of splenic hemangiosarcoma in which tissues were
submitted for histopathological evaluation. Mean sur-
vival time for these 22 cases was reported as 91 days.
Clinical stages of the disease were not reported, and
cases that died in the immediate postoperative period
were included in survival data calculations.
The primary purpose of this retrospective study
was to determine survival times for dogs with Stage I
or II splenic hemangiosarcomas that were treated by
splenectomy alone and survived the immediate post-
operative period. The secondary purpose was to de-
termine if the current staging system independently
predicted survival in this group of cases.
Materials and Methods
Medical records of 32 dogs seen at the Tufts Univer-
sity School of Veterinary Medicine (n=17) and the
Angell Memorial Animal Hospital (n=15) in the three-
year period (January 1, 1991 through December 31,
1993) were reviewed retrospectively. Cases were cho-
sen on the basis of a histopathological diagnosis of
hemangiosarcoma confined to the spleen (either in-
tact or ruptured) and no evidence of gross metastatic
disease. All cases were treated with surgery alone and
were excluded from the study if any adjunctive thera-
pies were given postoperatively. Only those cases
that survived the immediate postoperative period (i.e.,
seven days) were included. All histopathological
slides were reviewed, and the diagnoses were con-
firmed by one pathologist (Gliatto).
Details of age, breed, and sex were recorded along
with presenting history, duration of signs, and physi-
cal examination findings. Each case had a complete
blood count, serum biochemical profile, and thoracic
radiographs performed. Results of abdominal
radiographs (n=24), abdominal ultrasonographic ex-
amination (n=21), echocardiography (n=6), electro-
cardiography (n=19), or liver biopsy (n=20) were
recorded. Results of any additional adjunctive tests
were noted. Follow-up information was obtained
through telephone contact and a survey mailed to
owners and referring veterinarians. Owners confirmed
signalment and date of surgery. They also answered
questions regarding clinical signs following surgery,
current status (i.e., alive or dead) of the animal, and
date and cause of death.
Survival data was obtained from the medical
records and owner contact and was analyzed using
standard methods. Survival time was defined as the
interval from splenectomy until death. All computa-
tions were performed using a software package.
Kaplan-Meier product limit estimate of the survivor
function was generated, and standard parametric
survival distributions (i.e., exponential, Weibull,
Rayleigh, gamma, normal, and log normal) were fit-
ted to the data, with log normal calculated to meet the
goodness of fit criterion.
The Peto generalization of
Wilcoxon’s 2-sample rank sum test was used to ex-
amine the potential relationship between survival and
the following factors: Stage I or Stage II disease,
presenting signs of collapse or abdominal distention,
presence of anemia (i.e., hematocrit less than 32%),
DIC, postoperative ventricular arrhythmias, or ad-
ministration of a blood transfusion. The same test
also was used to assess the effects of gender and
Clinical Staging System for
Canine Hemangiosarcoma
T Primary tumor
T0—no evidence of tumor
T1—tumor confined to spleen
T2—tumor confined to spleen, but ruptured
T3—tumor invading adjacent structures
N Regional lymph nodes
N0—no regional lymph-node involvement
N1—regional lymph-node involvement
N2—distant lymph-node involvement
M Distant metastasis
M0—no evidence of distant metastasis
M1—distant metastasis
I—T0 or T1, NO, MO
II—T1 or T2, N0 or N1, M0
III—T2 or T3, N1 or N2, M1
September/October 1998, Vol. 34 Splenic Hemangiosarcoma 419
presence of anisocytosis, schistocytosis, or polychro-
masia on survival.
The effects of age, duration of
clinical signs, hematocrit, platelet count, presence
and number of nucleated red blood cells (nRBCs),
and white blood cell (WBC) count on survival also
were analyzed using the Cox proportional hazards
regression test.
Nineteen female (18 spayed, one intact) and 13 male
(five neutered, eight intact) cases were diagnosed with
Stage I (n=13) or Stage II (n=19) splenic heman-
giosarcomas. The median age was 10 years (range,
six to 14 years). Seven different breeds were repre-
sented, with the most common being mixed-breed
dog (n=10) followed by the golden retriever (n=7)
and German shepherd dog (n=7). Of the 19 cases
diagnosed with Stage II disease, all had rupture of
their primary tumors and none had regional lymph-
node involvement.
Clinical signs at presentation were similar among
the cases. An acute onset of lethargy or weakness
sometimes was accompanied by collapse (n=10). The
median duration of clinical signs was two days (range,
zero to 60 days). Signs in some cases waxed and
waned over several days, while other cases were pre-
sented to veterinarians at the first occurrence of clini-
cal signs. Two cases were without clinical signs, and
the splenic masses were incidental findings.
On the initial presenting physical examinations,
many cases had pale mucous membranes (n=17) or a
palpable abdominal mass (n=20). Presence of a
splenic mass was diagnosed or confirmed either by
abdominal radiography (n=24) or abdominal ultra-
sonography (n=21). Presence of abdominal fluid pre-
cluded the use of abdominal radiographs or made the
results of abdominal radiographs nondiagnostic in
some cases. One case had neither abdominal radiog-
raphy nor abdominal ultrasonography performed;
however, emergency surgery confirmed rupture of a
splenic mass.
The overall median hematocrit was 24% (range,
16% to 50%). Twenty-three of the cases were anemic
at presentation. Eleven cases received blood transfu-
sions, and one case received four additional
transfusions in the weeks following splenectomy. Ab-
normalities in red blood cell (RBC) morphology were
common. Anisocytosis (n=18) and nRBCs (n=15)
were observed most frequently; however, schisocytes
(n=6), acanthocytes (n=6), burr cells (n=6), poikilo-
cytes (n=5), spherocytes (n=3), Howell-Jolly bodies
(n=3), and target cells (n=1) also were present on
peripheral blood smears. The median platelet count
was 60,500/μl (range, 28,000 to 612,000/μl) at ad-
mission. Thirteen of the cases were thrombocytopenic
(platelet counts, less than 200,000/μl).
Coagulation profiles were performed in 19 cases.
Five cases were diagnosed with DIC based on meet-
ing three of the following five criteria: presence of
thrombocytopenia; fibrinogen degradation products
(FDPs) greater than 10 μ g/ml; prolongation of one or
more of the coagulation times by greater than 25% of
the control; presence of fragmented RBCs; and fi-
brinogen less than 80 mg/dl.
Cases diagnosed with
DIC were treated variably with heparin, whole blood,
fresh-frozen plasma, or a combination of these treat-
ments, and they were monitored continually by re-
peated coagulation profiles.
Preoperative electrocardiograms were performed
on 18 cases because of suspected cardiac arrhythmias.
Of the 18 cases, 12 had ventricular arrhythmias at-
tributable to their splenic disease and were treated
with intravenous lidocaine intraoperatively and post-
Two cases required additional oral
antiarrhythmic medication following discharge from
the hospital. Cardiac ultrasonographic examinations
were performed in six cases either because of
arrhythmias or suggestion of a cardiac abnormality
on thoracic radiographs; however, no ultrasono-
graphic evidence of a mass was noted in any case. In
one case, cardiac ultrasonographic examination con-
firmed a previously diagnosed dilatative cardiomy-
opathy that was responsive to treatment with digitalis
glycosides, and two other cases were diagnosed with
compensated mitral valvular regurgitation secondary
to valvular endocardiosis.
Nineteen cases had hemoperitoneum at surgery.
Liver biopsy was performed in 20 cases due to a
grossly abnormal appearance of the livers at surgery.
No evidence of metastatic disease was found in any
of the liver biopsies, and the most common histo-
pathological diagnosis was nodular hyperplasia. One
case had a mesenteric lymph-node biopsy with no
evidence of metastatic disease.
Follow-up information was obtained in 31 cases,
and all were dead at the time of reporting. One case
moved out of state 241 days following the splenec-
tomy and was lost to follow-up. Of the 31 cases with
follow-up, one case died 162 days postsplenectomy
from complications associated with surgical correc-
tion of gastric dilatation-volvulus. No gross meta-
static disease was observed at the time of surgery. Of
the remaining 30 cases, four cases died acutely and
the others were euthanized due to a return of clinical
signs (e.g., weakness, lethargy, collapse, abdominal
distention) presumed to be caused by tumor me-
tastases. No cases were necropsied, but one case had
evidence of hepatic metastases on abdominal ultra-
sonography at the time of euthanasia. The median
survival time was 86 days (mean, 116 days; range, 14
to 470 days) [Figure 1]. The one-year survival rate
was 6.25%. No variables were found to be significant
420 JOURNAL of the American Animal Hospital Association September/October 1998, Vol. 34
prognostic indicators of survival. Stage of disease did
not correlate with survival time in this group of cases.
Population characteristics and clinical histories were
very similar to those previously reported. The patient
group was composed primarily of older, large-breed
dogs with golden retrievers and German shepherd
dogs being the predominant purebreds represented.
The median survival time of 86 days (mean, 116
days) is longer than previously reported survival
In the absence of necropsies for these cases, the
authors have presumed that the cause of the deaths
was related to hemangiosarcoma in all but the case
which died during an unrelated surgery. The signs of
abdominal swelling and collapse were compatible
with hemangiosarcoma metastases and resultant
Hemangiosarcoma is an aggressive neoplasm
which appears to have microscopic metastases present
at the time of surgery and which causes death rapidly
in most affected dogs. Stage of disease did not seem
to be a predictor of survival. Cases in this study with
Stage II hemangiosarcoma did not have macroscopic
evidence of lymph-node metastases. It has been
thought previously that rupture of the tumor may re-
sult in “seeding” of the peritoneal cavity with meta-
static tumors. It appears that early in the course of the
disease, Stage I versus Stage II status does not affect
survival. However, under conditions of appropriate
treatment, long-term survival of splenic hemangiosar-
coma does appear to be affected by stage. In a recent
report comparing the use of liposome-encapsulated
muramyl tripeptide phosphatidylethanolamine (L-
MTP-PE) as an adjuvant therapy to splenectomy to
the use of combination chemotherapy (i.e., doxorubi-
cin and cyclophosphamide) as adjuvant therapy to
splenectomy, a significant difference in survival times
was found between cases with Stage I disease and
cases with Stage II disease. Cases with clinical Stage
I disease had significantly prolonged disease-free in-
tervals, less metastases, and longer overall survival
times as compared to cases with Stage II disease.
Lesions in the liver that were thought to be com-
patible with metastatic disease were seen at surgery
in 20 cases. Similar lesions were identified on ultra-
sonography. Histologically, most of these lesions were
hyperplastic nodules and none were metastatic le-
sions. Therefore, care should be taken in presumptive
diagnosis and staging without confirmatory histopa-
Despite the poor survival for most of these cases,
owners were pleased with their decisions to treat their
pets surgically. Quality of life was deemed good for
all cases following discharge from the hospital, and
all cases returned to normal health until the develop-
ment of clinical metastatic disease.
The biological behavior of hemangiosarcoma is a
pattern of micrometastases present at the time of sur-
gery. Since micrometastatic tumors generally are
thought to have a higher growth fraction resulting in
an increased sensitivity to most adjuvant therapies,
cases with splenic hemangiosarcoma theoretically
should benefit from postoperative chemotherapy. Che-
motherapy most likely is to be successful when the
total body burden of tumor is low or microscopic.
Adjuvant chemotherapy after surgical excision of vis-
ible tumor would appear to give the best prognosis
since it generally is accepted that grossly visible pri-
mary tumors are not curable with drug therapy alone.
Cases with Stage I or II splenic hemangiosarcoma
would appear to be suited ideally to receive adjuvant
chemotherapy, yet the role of commercially available
chemotherapy protocols have not been validated fully
in the treatment of this neoplasm. Reports of adjuvant
chemotherapy have included tumors in different loca-
tions and with different clinical stages and therefore
may represent very different manifestations of the
same histological disease.
Two similar chemotherapy protocols based on
doxorubicin and cyclophosphamide, with and without
the inclusion of vincristine, have been reported for
the treatment of hemangiosarcoma in dogs. In a group
treated with vincristine, doxorubicin, and cyclophos-
phamide (VAC protocol), the authors reported a me-
dian survival time of 124 days for three cases with
Stage II splenic disease. No cases with Stage I splenic
disease were treated.
Similar results were achieved when vincristine was
excluded from the treatment regime (i.e., doxorubicin
and cyclophosphamide, AC protocol). Four cases with
Figure 1—Kaplan-Meier survival curve duration estimates for
dogs with Stage I and Stage II splenic hemangiosarcoma treated
with surgery alone.
September/October 1998, Vol. 34 Splenic Hemangiosarcoma 421
Stage II hemangiosarcomas (both splenic and cutane-
ous) had a median survival time of 172 days. No
cases with Stage I splenic disease were treated.
taneous and subcutaneous hemangiosarcomas have
been shown to behave differently than visceral hem-
angiosarcomas, particularly when gross metastatic
disease is absent. Some cases have been reported to
have cutaneous hemangiosarcomas present 1.2 to 2.7
years before surgical removal. It also has been sug-
gested that hemangiosarcoma in the skin may repre-
sent a tumor associated with solar-induced changes,
accounting for its prolonged course.
Inclusion of
other less-aggressive forms of the disease within a
treatment group may imply that adjuvant therapies
are more efficacious than may be warranted.
Interestingly, when doxorubicin was used as a
single agent, 27 cases that were rendered free of vis-
ible hemangiosarcoma had a median survival time of
172 days. Twelve of these cases had primary splenic
disease, but their survival times were not reported
independent of other locations. Cases with cutaneous
tumors were not included in this treatment group.
Inclusion of locations other than the spleen in evalu-
ating survival times makes inferences regarding the
role of doxorubicin difficult, because this may repre-
sent different manifestations of the same disease.
The largest reported group of dogs with primary
splenic disease treated with commercially available
chemotherapeutics consisted of seven cases with
Stage I disease and nine cases with Stage II disease.
These cases were treated with the AC protocol as a
control to evaluate the role of L-MTP-PE in the treat-
ment of canine hemangiosarcoma. Median survival
time reported in the seven Stage I cases was 166 days,
and median survival time reported in the nine cases
with Stage II disease was 96 days.
Splenic hemangiosarcoma is an aggressive disease
that warrants a grave prognosis. No prognostic
factors for survival following splenectomy were iden-
tified. Complications (i.e., DIC or ventricular ar-
rhythmias) do not appear to affect the course of the
disease after the immediate postoperative period. Che-
motherapeutic drugs and immune-response modifiers
may improve survival times significantly in dogs with
hemangiosarcoma macroscopically confined to the
spleen; however, clinical trials with appropriate se-
lection of patients are needed to define the role of
such modalities clearly.
Number Cruncher statistical system, Version 5.5 - Survival Analysis; JL
Heintze, Kaysville, UT
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... Presenting blood work was evaluated for previously identified prognostic factors (thrombocytopenia and anemia) [27][28][29]. Thrombocytopenia was defined as an absolute platelet count <100,000/uL, and anemia as a hematocrit <32%, as previously described [30]. Adverse events were graded according to the VCOG v1.1 grading scale [31]. ...
Full-text available
Canine splenic hemangiosarcoma (HSA) is an aggressive tumor with a short overall survival time (OST) despite treatment with splenectomy and adjuvant doxorubicin. Modulation of the immune system has been shown to be effective for a variety of human tumors, and may be effective for canine tumors, including HSA. Immunocidin® is a non-specific immunotherapy based on a mycobacterial cell wall fraction. Preliminary work suggests Immunocidin® is safe to give intravenously (IV) in tumor-bearing dogs. This work aimed to evaluate the safety of doxorubicin and Immunocidin® combination in dogs with naturally occurring splenic HSA. A secondary aim of this study was to collect preliminary efficacy data to support a subsequent comprehensive, prospective clinical trial in canine patients with HSA, if the combination of doxorubicin and Immunocidin® was found to be safe. Eighteen dogs with stage II-III splenic HSA were recruited to receive 5 doses of sequential IV doxorubicin and Immunocidin® at two-week intervals following splenectomy. Adverse events (AEs) were graded according to the Veterinary Cooperative Oncology Group v1.1 (VCOG) scheme. Overall survival time was calculated from the date of splenectomy to date of death or loss to follow-up. AEs during administration were infrequent, the most common being hypertension. One patient developed limb and facial twitching and was removed from the study. After infusion, common AEs included lethargy, hyporexia, and diarrhea. One patient developed VCOG grade 5 diarrhea, thrombocytopenia, and anemia. Modifications in the treatment regimen were made to prevent these signs in subsequent patients. The median OST in dogs treated with the combination therapy was estimated at 147 days (range: 39-668 days). Although generally safe, the combination of doxorubicin and Immunocidin® appeared to cause more gastrointestinal effects than doxorubicin alone, and no apparent improvement in OST was noted in this population of dogs.
... However, survival times in dogs treated with PSP alone were similar to historical outcomes for patients undergoing splenectomy alone. 2,7,10 It is also worth noting that the survival times of dogs with splenic HSA treated with PSP alone following splenectomy in the current study evaluating a larger cohort of dogs is shorter than that reported in the earlier study by Brown and colleages. 15 There are several limitations of the current study. ...
Canine splenic hemangiosarcoma (HSA) is an aggressive tumor of vascular endothelium that carries a grave prognosis following standard of care treatment with surgery and doxorubicin. A previous pilot study revealed potential anti‐tumor activity of I'm Yunity® Polysaccharopeptide (PSP) for canine HSA. The aim of this prospective study was to assess patient outcome when treated with PSP alone or in combination with doxorubicin post‐splenectomy compared to patients treated with surgery and doxorubicin that received a placebo in place of PSP. Dogs undergoing splenectomy for splenic HSA were eligible. Following splenectomy, owners were offered treatment with PSP alone or adjuvant doxorubicin chemotherapy(unblinded). Patients with owners that selected to proceed with doxorubicin chemotherapy were blindly randomized to receive placebo or PSP. Dogs were evaluated weekly for 15 weeks, then scheduled for monthly visits until death. One hundred and one dogs were included in the final analysis: 51 PSP alone, 25 doxorubicin/placebo, and 25 combination PSP/doxorubicin. On multivariate analysis, dogs treated with single agent PSP, female dogs, decreased hematocrit at diagnosis, and stage III disease were negatively significantly associated with outcome; however, an interaction between treatment group and sex was documented. Gender‐specific outcomes revealed no significant difference in survival between treatment groups for male dogs, but female dogs treated with PSP alone had significantly reduced survival compared to females receiving doxorubicin/placebo (HR 0.21; p = 0.004). Anemia (HR 5.28; p < 0.001) and stage III disease (HR 2.9; p = 0.014) remained negatively associated with survival when controlling for sex and treatment group. The addition of PSP to doxorubicin post‐splenectomy did not improve survival in dogs with splenic HSA.
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Background Canine hemangiosarcoma (HSA) is an aggressive cancer of endothelial cells associated with short survival times. Understanding the genomic landscape of HSA is critical to developing more effective therapeutic strategies. Objectives To determine the relationships between genomic and clinical features including treatment and outcome in canine splenic HSA. Animals 109 dogs with primary splenic HSA treated by splenectomy that had tumor sequencing via the FidoCure® Precision Medicine Platform targeted sequencing panel. Methods Patient signalment, weight, metastasis at diagnosis, treatment, and survival time were retrospectively evaluated. The incidence of genomic alterations in individual genes and their relationship to patient variables and outcome were assessed. Results Somatic mutations in TP53 (n = 45), NRAS (n = 20), and PIK3CA (n = 19) were most common. Survival was associated with metastases at diagnosis, germline variants in SETD2 and NOTCH1 , and nominally with breed. Age at diagnosis was associated with NRAS mutations and breed. TP53 and PIK3CA mutations were found in larger dogs, germline SETD2 variants in smaller dogs. Doxorubicin (DOX) treatment did not significantly improve survival time, while targeted therapies had a significant early survival benefit. Conclusions and clinical importance DOX treatment may provide limited clinical benefit for dogs with splenic HSA, while targeted therapy may provide early survival benefit. Genetic signatures associated with splenic HSA may be useful in guiding targeted therapy to improve outcomes. Germline variants, age, size, and breed may be useful prognostic factors and provide insight into the genomic landscape of the tumor.
OBJECTIVE To determine if clinician experience influenced the euthanasia rate in 2 common surgical emergencies. ANIMALS 142 dogs with nontraumatic hemoabdomen (NTH) due to suspected ruptured splenic mass and 99 dogs with gastric dilatation-volvulus (GDV) where the owner either elected surgery or euthanasia. PROCEDURES Medical records were reviewed for dogs that had either NTH or GDV. For each patient, the owner’s decision to pursue euthanasia versus surgery was recorded. The primary clinician was categorized as an intern, defined as a clinician with < 12 months experience, or a non-intern, defined as a clinician with more than 12 months experience. The euthanasia rates were compared used a Fisher exact, and the 95% CI was calculated for the risk of euthanasia if the primary clinician was an intern compared with a non-intern. If a difference was identified, subgroups comparing time of day, referral status, age, Hct, total solids, lactate, and heart rate were evaluated using a t test with a Bonferroni correction for the continuous variables and a Fisher exact for categorical variables. RESULTS For dogs with NTH, the euthanasia rate for cases primarily managed by non-interns (52%) was significantly lower than that of interns (76%; P = .005). The relative risk of euthanasia associated with NTH when the case was treated by an intern was 1.44 with a 95% CI of 1.1229 to 1.8567. For 99 dogs with GDV, the rate of euthanasia was not different between interns and non-interns. CLINICAL RELEVANCE The euthanasia rate for dogs with NTH may be impacted by the level of experience of the clinician. Support of new clinicians during challenging conversations should be provided.
For virtually all malignant solid tumors, lymph node status is one of the most important prognostic indicators of poor survival and governs therapeutic options, as established in human oncology. There are two interconnected portions of the hemolymphatic circulatory pathway. One portion is the better understood hemovascular circulation composed of arteries and veins and the heart. The other is the unidirectional lymphatic circulation. The spleen functions as a compliant vastly expansile structure of the circulatory system, serving as a physiology reserve feature or compensatory mechanism for blood loss. Patients require careful monitoring following splenectomy. The thymus incompletely atrophies, the start of which coincides with the loss of deciduous teeth in the dog. Canine tonsillar squamous cell carcinoma is the primary tonsillar carcinoma in dogs. Cats tend to present with advanced inoperable tonsillar squamous cell carcinoma. Progressive lymph node metastasis found in five dogs were excised in four dogs and one dog receiving only radiation therapy.
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OBJECTIVE To assess the diagnostic value of the ultrasonographic description of a splenic mass or nodule as cavitated in dogs with nontraumatic hemoabdomen. ANIMALS 106 dogs with a nontraumatic hemoabdomen that underwent abdominal ultrasonography and splenectomy with histologic examination of splenic lesions between 2005 and 2018. PROCEDURES Medical records were reviewed for abdominal ultrasonographic and histologic findings. Diagnostic performance of ultrasonographic description of a splenic mass or nodule as cavitated as evidence of hemangiosarcoma or any malignancy was evaluated. RESULTS Ultrasonographic description of splenic lesions as cavitated had poor diagnostic utility in predicting presence of hemangiosarcoma or malignancy. Sensitivity and specificity of this test were 41.9% (95% CI, 30.5% to 54.3%) and 51.2% (95% CI, 36.8% to 65.4%), respectively, for detecting hemangiosarcoma, with positive and negative predictive values of 55.3% (95% CI, 41.2% to 68.6%) and 37.9% (95% CI, 26.6% to 50.8%), respectively. Results were similar for detecting malignancy. Cavitated lesions outside of the spleen were too rare for statistical analysis to be of value. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that relying on ultrasonographic description of cavitation to diagnose splenic lesions as malignant in dogs with nontraumatic hemoabdomen is unfounded. Other preoperative diagnostic tests may be more valuable in determining short- and long-term prognoses.
Objective The objectives of this study are (1) to determine whether there is an association between dogs with splenic hemangiosarcoma (HSA) and the development of premature ventricular contractions (VPCs), (2) to determine if there is a higher likelihood for dogs with ruptured splenic masses to be diagnosed with HSA and to develop VPCs, (3) to determine if the development of VPCs affects median survival times compared to dogs with benign or non-HSA malignant splenic masses. Study Design Retrospective case series. Animals Forty-five dogs. Methods Medical records of dogs undergoing splenectomy were reviewed for signalment, perioperative electrocardiogram (ECG), hematological values, histologic diagnosis, metastasis, and survival times. ECGs were performed preoperatively, intraoperatively, and continuously postoperatively. The presence of VPCs was recorded. The data were evaluated for an association between the development of VPCs and the histologic diagnosis of HSA. Results Eighteen out of 45 (40%) dogs were diagnosed with HSA with 13/18 (72%) dogs having VPCs postoperatively (P = .02). Ruptured splenic HSA and VPCs were noted in 13 dogs (P = .73). An association between dogs with and without VPCs diagnosed with HSA and median survival times could not be established. Conclusion Postoperative VPCs were more likely with splenic HSA. Splenic masses were more likely to be HSA if ruptured but less likely to develop VPCs. Development of VPCs does not affect median survival times. Clinical Significance Development of postoperative VPCs may be a potential indicator of HSA, however, this warrants further investigations. Development of VPCs does not have a deleterious effect on survival.
Introduction: Haemangiosarcoma (HSA) is a malignant neoplasm of dogs and cats that is suspected to originate from a pluripotent bone marrow progenitor with a complex and multifactorial pathogenesis. Approach: Pertinent literature was identified, reviewed, and summarized for inclusion in the manuscript. Results/interpretation: Dogs are more frequently diagnosed with HSA than cats, and primary sites of this disease include dermal, subcutaneous/intramuscular, and visceral (most commonly the spleen). Dogs and cats with HSA generally have a poor prognosis owing to the rapid and widespread metastasis typically associated with this disease. However, some forms such as cutaneous HSA behave in a less aggressive fashion with improved outcomes. Surgical excision and anthracycline-based chemotherapy remain the mainstays of treatment, although novel treatment modalities are currently under investigation for potential roles in treatment of this disease. Conclusion: This review aims to describe the clinical presentation and progression of the various forms of HSA in dogs and cats as well as to provide a systematic review of the veterinary literature with a focus on the various published treatment options and associated outcomes.
Objective: To develop a multivariable model and online decision-support calculator to aid in preoperative discrimination of benign from malignant splenic masses in dogs. Animals: 522 dogs that underwent splenectomy because of splenic masses. Procedures: A multivariable model was developed with preoperative clinical data obtained retrospectively from the records of 422 dogs that underwent splenectomy. Inclusion criteria were the availability of complete abdominal ultrasonographic examination images and splenic histologic slides or histology reports for review. Variables considered potentially predictive of splenic malignancy were analyzed. A receiver operating characteristic curve was created for the final multivariable model, and area under the curve was calculated. The model was externally validated with data from 100 dogs that underwent splenectomy subsequent to model development and was used to create an online calculator to estimate probability of splenic malignancy in individual dogs. Results: The final multivariable model contained 8 clinical variables used to estimate splenic malignancy probability: serum total protein concentration, presence (vs absence) of ≥ 2 nRBCs/100 WBCs, ultrasonographically assessed splenic mass diameter, number of liver nodules (0, 1, or ≥ 2), presence (vs absence) of multiple splenic masses or nodules, moderate to marked splenic mass inhomogeneity, moderate to marked abdominal effusion, and mesenteric, omental, or peritoneal nodules. Areas under the receiver operating characteristic curves for the development and validation populations were 0.80 and 0.78, respectively. Conclusions and clinical relevance: The online calculator ( or developed in this study can be used as an aid to estimate the probability of malignancy in dogs with splenic masses and has potential to facilitate owners' decisions regarding splenectomy.
The records of 73 dogs with splenic masses were evaluated retrospectively to determine whether ventricular arrhythmias, in the absence of clinically apparent underlying heart disease, were a common clinical finding. Associated clinical, laboratory, and pathologic findings were evaluated to search for clinical predictors of ventricular arrhythmias. Age, breed, weight, sex, coagulation abnormalities, electrolyte abnormalities, and hemoabdomen were unrelated to the development of arrhythmias (p > 0.05). Anemia was associated with the presence of arrhythmias (p = 0.005). Myocardial necrosis (10/18) and metastatic hemangiosarcoma (3/18) were common myocardial histopathologic findings. Proposed causes for arrhythmias in dogs with splenic masses include myocardial metastases, tissue hypoxia secondary to anemia or hypovolemia, and local or systemic catecholamine release.
Part I: Molecular Biology of Cancer Molecular Methods in Oncology Section 1. Amplification Techniques Section 2. RNA Interference Section 3. cDNA arrays Section 4. Tissue arrays Section 5. Cytogenetics Section 6. Bioinformatics Genomics and Proteomics Molecular Targets in Oncology Section 1. Signal transduction systems Section 2. Cell cycle Section 3. Apoptosis Section 4. Telomerase Invasion and Metastases Angiogenesis Cancer Immunology Part II: Principles of Oncology Etiology of Cancer: Viruses Section 1. RNA Viruses Section 2. DNA Viruses Etiology of Cancer: Chemical Factors Etiology of Cancer: Tobacco Etiology of Cancer: Physical Factors Epidemiology of Cancer Section 1. Epidemiologic Methods Section 2. Cancer Statistics Principles of Surgical Oncology Section 1. General Issues Section 2. Laparascopic Surgery Principles of Radiation Oncology Principles of Medical Oncology Pharmacology of Cancer Chemotherapy Section 2. Pharmocokinetics Section 3. Pharmacogenomics Section 4. Alkylating Agents Section 5. Cisplatin and its Analogues Section 6. Antimetabolites Section 7. Topoisomerase Interactive Agents Section 8. Antimicrotubule Agents Section 9. Miscellaneous Chemotherapeutic Agents Pharmacology of Cancer Biotherapeutics Section 1. Interferon Section 2. Interleukin 2 Section 3. Histone deacetylase inhibitors as differentiation agents Section 4. Monoclonal Antibodies Pharmacology of Endocrine Manipulation Design and Analysis of Clinical Trials Part III: Practice of Oncology Cancer Prevention: Preventing Tobacco-Related Cancers Cancer Prevention: Diet and Chemopreventive Agents Section 1. Dietary fat Section 2. Dietary Fiber Section 3. Dietary fruits and vegetables: naturally occurring anticarcinogens Section 4. Retinoids, carotenoids and micronutrients Section 5. Dietary Carcinogens Section 6. Cyclo-oxygenase inhibitors Section 7. Physical Activity and Body Weight Cancer Prevention: Role of Surgery in Cancer Prevention Cancer Screening Advanced Molecular Diagnostics Advanced Imaging Methods Section 1. Functional and Metabolic Imaging Section 2. Interventional Radiology Cancer Diagnosis: Endoscopy Section 1. Gastrointestinal endoscopy Section 2. Respiratory Tract Cancer of the Head and Neck Section 1. Molecular Biology of Head and Neck Tumors Section 2. Treatment of Head and Neck Cancers Section 3. Rehabilitation after Treatment for Head Cancer of the Lung Section 1. Molecular Biology of Lung Cancer Section 2. Non-small Cell Lung Cancer Section 3. Small Cell Lung Cancer Neoplasms of the Mediastinum Cancers of the Gastrointestinal Tract
Fifteen dogs with hemangiosarcoma were treated with a combination of vincristine, doxorubicin, and cyclophosphamide after incisional or excisional biopsy. The median survival for all fifteen dogs was 172 days (mean survival = 316 days). The median survival for those dogs with splenic hemangiosarcoma was 145 days (mean survival = 271 days) as compared with previously published median survival times in dogs with splenic hemangiosarcoma treated with surgery alone of 19 to 65 days. Toxicities included neutropenia (11/15), severe gastroenteritis (4/15), cardiotoxicity (3/15), and sepsis (2/15). The authors conclude that vincristine, doxorubicin, and cyclophosphamide chemotherapy may be an efficacious treatment modality in dogs with hemangiosarcoma and is associated with acceptable toxicity.
The relationship between skin pigmentation and piliation and the development of hemangiomas and hemangiosarcomas in the dermis and subcutaneous tissue was studied in 212 dogs. These 212 dogs had a combined total of 306 tumors; 38 of these 212 dogs had two or more of the same tumor in a different location or a combination of hemangioma and hemangiosarcoma. The average age of the dogs at the time of excision of these tumors was greater than 10 years. There was no sex predilection for the presence or absence of tumors. Cutaneous hemangiomas (73%) were more common than cutaneous hemangiosarcomas (27%). Hemangiomas had no predilection for dermis (51%) or subcutaneous tissue (47%), but hemangiosarcomas had a marked predilection for dermis (73%) over subcutaneous tissue (7%). Dogs with short hair coats and lightly pigmented skin had more hemangiomas and hemangiosarcomas of the dermis (65%) than did dogs with variable length hair coats and pigmentation (28%). Dogs with short hair coats and lightly pigmented skin had fewer hemangiomas and hemangiosarcomas of the subcutaneous tissue (10%) than did dogs with variable length hair coats and pigmentation (22%). Dogs with short hair coats and lightly pigmented skin also had more hemangiomas and hemangiosarcomas of ventral glabrous skin (65%) than did dogs with variable length hair coats and pigmentation (22%). In addition, there was no predilection of subcutaneous hemangiosarcoma for haired (33%) versus glabrous (33%) skin, but dermal hemangiosarcoma had a marked predilection for the glabrous skin (63%) when compared with haired skin (10%). The increased incidence of dermal hemangiomas and hemangiosarcomas in ventral glabrous skin suggests an association between solar radiation and the biologic properties of glabrous skin in the genesis of these tumors.
The prevalence data of splenic diseases from 3 sources were studied. Group 1 consisted of a general diagnostic survey of accessions submitted from private veterinary hospitals in California during a period of approximately 4 years and included 1,372 submissions of canine splenic tissue. Group 2 consisted of surgical splenectomy specimens from 92 dogs; the specimens were submitted to the laboratory for gross and histologic evaluation prior to fixation, and a questionnaire was subsequently sent to determine the outcome of the disease. Group 3 was made up of specimens of 105 splenic lesions derived from a large colony of Beagles with complete medical records and records of pathologic findings. In this study, splenic hematoma and hyperplastic nodule, not hemangiosarcoma, made up the bulk of splenic lesions. Hemangiosarcoma was the most frequent neoplasm of the canine spleen, but the combined prevalence of all other splenic neoplasms was similar to that of hemangiosarcoma alone. Splenic hematoma and hemangiosarcoma were grossly indistinguishable in most cases. Hyperplastic lymphoid nodules and hematomas of the spleen appeared to represent a continuum. If that finding was correlated with microscopic splenic blood flow, specific causal relationship could be suggested. Prognostically, the live/dead ratio and mean survival of dogs with various splenic lesions differed significantly.