Characterization of tropical tree rhizobia and description of Mesorhizobium plurifarium sp. nov. Int J Syst Bacteriol

University of Helsinki, Helsinki, Uusimaa, Finland
International journal of systematic bacteriology (Impact Factor: 2.27). 04/1998; 48 Pt 2(2):369-82. DOI: 10.1099/00207713-48-2-369
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A collection of strains isolated from root nodules of Acacia species in Senegal was analysed previously by electrophoresis of total cell protein, auxanographic tests, rRNA-DNA hydridization, 16S rRNA gene sequencing, DNA base composition and DNA-DNA hybridization [de Lajudie, P., Willems, A., Pot, B. & 7 other authors (1994). Int J Syst Bacteriol 44, 715-733]. Strains from Acacia were shown to belong to two groups, Sinorhizobium terangae, and a so-called gel electrophoretic cluster U, which also included some reference strains from Brazil. Further taxonomic characterization of this group using the same techniques plus repetitive extragenic palindromic-PCR and nodulation tests is presented in this paper. Reference strains from Sudan and a number of new rhizobia isolated from nodules of Acacia senegal, Acacia tortilis subsp. raddiana and Prosopis juliflora in Senegal were included. As a result of this polyphasic approach, the creation of a new species, Mesorhizobium plurifarium, is proposed for a genotypically and phenotypically distinct group corresponding to the former cluster U and containing strains isolated from Acacia, Leucaena, Prosopis and Chamaecrista in West Africa (Senegal), East Africa (Sudan) and South America (Brazil). The type strain of Mesorhizobium plurifarium ORS 1032 has been deposited in the LMG collection as LMG 11892.

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Available from: Fatima Maria de Souza Moreira, Jan 04, 2014
    • "The N 2 -fixing capacity of a legume tree is often used to explain its ability to grow better on and restore the fertility of Ndepleted soil (Dommergues 1995). S. Senegal is a promiscuous species that could be nodulated with various rhizobial taxa and strains (Bakhoum et al. 2014; de Lajudie et al. 1998; Fall et al. 2008; Nick et al. 1999; Njiti and Galiana 1996; Odee et al. 1995; Odee et al. 1997; Sarr et al. 2005b). Nevertheless, previous studies have shown that S. Senegal is mainly nodulated in Senegal by rhizobial strains phylogeneticaly close to Mesorhizobium plurifarium (Bakhoum et al. 2014; Fall et al. 2008; Sarr et al. 2005b). "
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    ABSTRACT: Aims The focus of the study was to determine the symbiotic and growth response of three Senegalia Senegal (Syn. Acacia Senegal, gum arabic tree) provenances, namely Dahra (Senegal), Tera (Niger) and Makueni (Kenya) to inoculation with selected S. Senegal-nodulating rhizobia in soils from Dahra and Goudiry regions of Senegal, representing typical soil and enviromental conditions for establishing gum arabic production plantations. Methods A greenhouse experiment was performed to evaluate the effect of 11 rhizobial strains on nodulation and growth of three S. Senegal provenances in two field soils, differing in nutrient status and indigenous rhizobia. After 4 months, plants were harvested for determination of nodulation, shoot and root dryweight. Results Nodulation and growth of S. Senegal varied in relation to rhizobial strain, provenance, soil type, and their interactions. Generally, nodulation was higher in Dahra than Goudiry soils, while Makueni provenance was the most compatible host. Inoculation had a significant effect on all parameters measured in Dahra field soil. By contrast, inoculation had a significant effect on height (shoot length), and shoot, root and total dry matter but not on nodulation. In the two field soils, seed provenance effect was significant for all parameters measured. The interaction between inoculation and provenance showed a significant effect on all parameters measured except nodule number in Dahra field soil while in Goudiry, the interaction had a significant effect on seedling height and shoot, root, and total dry matter but this effect was not significant with nodulation parameters. Conclusions S. Senegal is variable in its response to inoculation, it is therefore advantageous to select and match effective rhizobia-provenance symbionts for each site.
    No preview · Article · Sep 2015 · Plant and Soil
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    • "We report here that while the isolates 10L4.2 and 10L5.3 clustered close to the type strain M. plurifarium LGM 11892 that was known to nodulate Acacia spp. and L. leucocephala [43], [44]; isolate 10L11.4 proved to be related to the previously reported R. alamii GBV016T (isolated from the rhizosphere of Arabidopsis thaliana) [45] and CCBAU15292 (isolated from Albizia julibrissin) [46], and to R. mesosinicum CCBAU25010 (isolated from Glycine soja) [47]. "
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    ABSTRACT: Desmanthus paspalaceus (Lindm.) Burkart belongs to the D. virgatus complex, subfamily Mimosoidae. The known potential as livestock fodder of several of these legumes prompted us to undertake a phenotypic, molecular, and symbiotic characterization of the D. paspalaceus symbionts in the Santa Fe province, Argentina. The rhizobia collected-containing isolates with different abiotic-stress tolerances-showed a remarkable genetic diversity by PCR fingerprinting, with 11 different amplification profiles present among 20 isolates. In selected isolates 16S-rDNA sequencing detected mesorhizobia (60%) and rhizobia (40%) within the collection, in contrast to the genus of the original inoculant strain CB3126-previously isolated from Leucaena leucocephala-that we typified here through its 16S rDNA as Sinorhizobium terangae. The results revealed the establishment by diverse bacterial genera -rhizobia, sinorhizobia, and mesorhizobia- of full N2-fixing symbiotic associations with D. paspalaceus. This diversity was paralleled by the presence of at least two different nodC allelic variants. The identical nodC alleles of the Mesorhizobia sp. 10.L.4.2 and 10.L.5.3 notably failed to group within any of the currently described rhizo-/brady-/azorhizobial nodC clades. Interestingly, the nodC from S. terangae CB3126 clustered close to homologs from common bean nodulating rhizobia, but not with the nodC from S. terangae WSM1721 that nodulates Acacia. No previous data were available on nod-gene phylogeny for Desmanthus symbionts. A field assay indicated that inoculation of D. paspalaceus with the local Rhizobium sp. 10L.11.4 produced higher aerial-plant dry weights compared to S. teranga CB3126-inoculated plants. Neither the mesorhizobia 10.L.4.2 or 10.L.5.3 nor the rhizobium 10L.11.4 induced root nodules in L. leucocephala or P. vulgaris. The results show that some of the local isolates have remarkable tolerances to several abiotic stresses including acidity, salt, and temperature; while exhibiting prominent N2 fixation; thus indicating suitability as candidates for inoculation of D. paspalaceus.
    Full-text · Article · Aug 2014 · PLoS ONE
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    • "Ensifer isolates with 16S rRNA gene sequence similarities over 99.6 % to the type strains of Ensifer mexicanus (Lloret et al., 2007), Ensifer terangae or Ensifer saheli (Delajudie et al., 1994) were obtained only from a single site (Ocuilan de Juárez) with alkaline soils (pH 8) while all other nodule isolates were from acid soils (Table S1). A sequence affiliated with members of the genus Mesorhizobium with 99.8 % sequence similarity to Mesorhizobium plurifarium LMG 11892 T (de Lajudie et al., 1998) was obtained from Laguna Bélgica. "
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    ABSTRACT: Calliandra grandiflora has been used as a medicinal plant for thousands of years in Mexico. Rhizobial strains were obtained from root nodules of C. grandiflora collected from different geographical regions in Chiapas and characterized by BOX-PCR, ARDRA and 16S rRNA gene sequences. Most isolates corresponded to Rhizobium and those not related to described species were further characterized by recA, atpD, rpoB and nifH gene phylogenies, phenotypic and DNA-DNA hybridization analyses. Three new, related Rhizobium species within the 'Rhizobium tropici group' share the same symbiovar that may be named sv. calliandrae. The type strains are for Rhizobium calliandrae CCGE524T (=ATCC BAA-2435T =CIP110456T =LBP2-1T), Rhizobium mayense CCGE526T (=ATCC BAA-2446T =CIP110454T =NSJP1-1T) and Rhizobium jaguaris CCGE525T (=ATCC BAA-2445T =CIP110453T =SJP1-2T).
    Preview · Article · Apr 2013 · International Journal of Systematic and Evolutionary Microbiology
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