Bullera penniseticola sp. nov. and Kockovaella sacchari sp. nov., two new yeast species isolated from plants in Thailand

Japan Collection of Microorganisms, Institute of Physical and Chemical Research(RIKEN), Saitama, Japan.
International journal of systematic bacteriology (Impact Factor: 2.27). 08/1998; 48 Pt 3(3):1025-30. DOI: 10.1099/00207713-48-3-1025
Source: PubMed


Two strains of ballistocondium-forming yeasts, isolated from plants collected in the south-east seacoast of Bangkok, Thailand, were described. The strains (K-272(T) and K-337(T)) were assigned to the genera Bullera and Kockovaella, respectively, based on morphological and chemotaxonomical characteristics. Phylogenetically, strain K-272(T) is close to Bullera hannae, and strain K-337(T) is close to Kockovaella thailandica and Kockovaella imperatae. These two strains represent new species based on DNA-DNA reassociation experiments. Bullera penniseticola Takashima et Nakase sp. nov. and Kockovaella sacchari Takashima et Nakase sp. nov. are proposed for K-272(T) (=JCM 9857(T)) and K-337(T) (=JCM 9858(T)), respectively.

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    • "[5] renamed the species as Cryptococcus hungaricus. However classical and molecular characterization (internal transcribed sequences and 5.8S rRNA, D1/D2 region of 26S rRNA and partial sequences of the 18S rRNA genes) of the six collection strains showed ¢ve of them to be relatives of the closely related Bullera genus [2] and one to be an isolate belonging to the distantly related Cysto¢lobasidiales order. Takashima et al. [6] have amended the genus Dioszegia to include both ballistoconidium-forming and non-ballistoconidium-forming yeasts and redescribed C. hungaricus species as D. hungarica. "
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    ABSTRACT: The organization of mitochondrial DNA was investigated in the six collection strains of the basidiomycetous yeast Dioszegia hungarica (Cryptococcus hungaricus) isolated so far. Physical and partial functional maps were constructed. Two strains (CBS 6324 and 6576) were identical while three others (CBS 4214, 5124, 6953) differed not only in the distribution of restriction sites but in gene order as well. Results confirm the hypothesis that these five strains are representatives of different closely related species. The sixth strain CBS 6569 revealed a unique mitochondrial genome organization. Its mtDNA separated into eight bands on agarose gel without enzymatic digestion. These molecules carried mitochondrial genes, and RFLP analysis of the four largest molecules using frequently-cutting restriction enzymes (KpnI and SmaI) showed them to have strongly homologous sequences. This unique mtDNA organization was also observed in a strain of Cystofilobasidium capitatum, providing evidence that CBS 6569 belongs to the Cystofilobasidium clade.
    Full-text · Article · Jul 2002 · FEMS Microbiology Letters
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    • "Nakase) and his coworkers isolated numerous strains of ballistoconidium-forming yeasts and described two new species of the genus Bensingtonia Ingold emend. Nakase & Boekhout (Takashima et al., 1995 ; Fungsin et al., 2001), one new species of the genus Bullera Derx (Takashima & Nakase, 1998), three new species of the genus Kockovaella Nakase, Banno & Y. Yamada (Nakase et al., 1991 ; Takashima & Nakase, 1998) and four new species of the genus Sporobolomyces Kluyver & van Niel (Takashima & Nakase, 2000). In this study, we isolated three strains of the genus Kockovaella from leaves collected in a tropical rain forest in Thailand. "
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    ABSTRACT: One yeast strain characterized by the proliferation of non-ballistosporous stalked conidia, budding cells and ballistoconidia, the presence of xylose in whole-cell hydrolysates, the presence of Q-10 as the major ubiquinone isoprenologue, the inability to ferment sugars and positive diazonium blue B (DBB) and urease reactions was isolated from a plant sample collected in a tropical rain forest in Thailand. The isolate clustered with Kockovaella species in the 18S rDNA-based tree. On the basis of the morphological, biochemical and molecular phylogenetic characteristics, the isolate was assigned to the genus Kockovaella. DNA complementarity experiments showed that the isolate was genetically distinct from known species of the genus Kockovaella. The isolate is described as Kockovaella barringtoniae sp. nov. The type strain is strain TY-278T (= JCM 10998T = TISTR 5770T).
    Preview · Article · Feb 2002 · International Journal of Systematic and Evolutionary Microbiology

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